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Protein

Terminal uridylyltransferase 4

Gene

Zcchc11

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay. Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem cell pluripotency (PubMed:19703396). Does not bind RNA directly, but recruited to RNA targets by RNA-binding protein LIN28A. Also catalyzes the 3' uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression (PubMed:19701194). May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult.By similarity3 Publications

Catalytic activityi

UTP + RNA(n) = diphosphate + RNA(n+1).1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1026 – 10261Magnesium or manganese; catalyticBy similarity
Metal bindingi1028 – 10281Magnesium or manganese; catalyticBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri930 – 94718CCHC-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1310 – 132718CCHC-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1358 – 137518CCHC-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • poly(A) RNA binding Source: MGI
  • RNA uridylyltransferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cytokine production Source: MGI
  • interleukin-6-mediated signaling pathway Source: UniProtKB
  • miRNA catabolic process Source: UniProtKB
  • miRNA metabolic process Source: UniProtKB
  • negative regulation of NF-kappaB transcription factor activity Source: MGI
  • positive regulation of interleukin-6 production Source: UniProtKB
  • pre-miRNA processing Source: UniProtKB
  • regulation of lipopolysaccharide-mediated signaling pathway Source: MGI
  • RNA 3'-end processing Source: UniProtKB
  • stem cell population maintenance Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

RNA-mediated gene silencing

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Terminal uridylyltransferase 4 (EC:2.7.7.52)
Short name:
TUTase 4
Alternative name(s):
Zinc finger CCHC domain-containing protein 11
Gene namesi
Name:Zcchc11
Synonyms:Kiaa0191, Tut4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2445126. Zcchc11.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm 1 Publication

  • Note: Mainly cytoplasmic (PubMed:19703396). Translocates into the cytoplasm following treatment of the cell with LPS (By similarity).By similarity1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • nucleolus Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi326 – 3261C → A: No effect on basal uridylation activity, but loss of LIN28A-enhanced uridylation; when associated with A-329. 1 Publication
Mutagenesisi329 – 3291C → A: No effect on basal uridylation activity, but loss of LIN28A-enhanced uridylation; when associated with A-326. 1 Publication
Mutagenesisi1026 – 10261D → A: Loss of nucleotidyltransferase activity; when associated with A-1028. 1 Publication
Mutagenesisi1028 – 10281D → A: Loss of nucleotidyltransferase activity; when associated with A-1026. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16441644Terminal uridylyltransferase 4PRO_0000385330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei102 – 1021PhosphoserineBy similarity
Modified residuei131 – 1311PhosphoserineCombined sources
Modified residuei176 – 1761PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiB2RX14.
MaxQBiB2RX14.
PaxDbiB2RX14.
PRIDEiB2RX14.

PTM databases

iPTMnetiB2RX14.
PhosphoSiteiB2RX14.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000034610.
ExpressionAtlasiB2RX14. baseline and differential.
GenevisibleiB2RX14. MM.

Interactioni

Subunit structurei

Interacts with LIN28A in the presence of pre-let-7 RNA. Interacts with T2BP.By similarity

Protein-protein interaction databases

BioGridi230978. 1 interaction.
DIPiDIP-48571N.
STRINGi10090.ENSMUSP00000044836.

Structurei

3D structure databases

ProteinModelPortaliB2RX14.
SMRiB2RX14. Positions 381-725, 978-1286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini649 – 69850PAP-associated 1Add
BLAST
Domaini1201 – 125454PAP-associated 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1425 – 1598174Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-B-like family.Curated
Contains 3 CCHC-type zinc fingers.PROSITE-ProRule annotation
Contains 2 PAP-associated domains.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri930 – 94718CCHC-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1310 – 132718CCHC-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1358 – 137518CCHC-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2277. Eukaryota.
COG5260. LUCA.
GeneTreeiENSGT00550000074490.
HOGENOMiHOG000231134.
InParanoidiB2RX14.
KOiK13291.

Family and domain databases

Gene3Di4.10.60.10. 4 hits.
InterProiIPR002058. PAP_assoc.
IPR002934. Polymerase_NTP_transf_dom.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF03828. PAP_assoc. 2 hits.
PF00098. zf-CCHC. 2 hits.
[Graphical view]
SMARTiSM00343. ZnF_C2HC. 3 hits.
[Graphical view]
SUPFAMiSSF57756. SSF57756. 2 hits.
PROSITEiPS50158. ZF_CCHC. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2RX14-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEPKTSKNE NHEPKKNIIC EESKAVKIIS NQTLKPRNDK SEIGTSSLNR
60 70 80 90 100
NSSKKTKQND ICIEKTEAKS CKVNAASVPG PKDLGLVHRD QSHCKMKKLP
110 120 130 140 150
NSPMKAQKGS SQTKLEKTPS LQTKAEKVPK SPNLPVKAEK APCTTAEATT
160 170 180 190 200
EKALNSQRKE ENTPTSQMKL QKTPRSPLEP ENVPSLLLKE NVKQTESQQT
210 220 230 240 250
GKKLTSSFVS MDKRKSEALQ GEKSALENSS LSQKQQTQTD NIADSDDSAS
260 270 280 290 300
GIEDTADDLS KMKSEESNKE NSSEMDYLEN ATVIDESALT PEQRLGLKQA
310 320 330 340 350
EERLERDHIF RLEKRSPEYT NCRYLCKLCL IHIENIQGAH KHIKEKRHKK
360 370 380 390 400
NILEKQEESE LRSLPSPSSA HLAALSVAVV ELAKEQGITD DDLRIRQDIV
410 420 430 440 450
EEMSKVIMTF LPECSLRLYG SSLTKFALKS SDVNIDIKFP PKMNHPDLLI
460 470 480 490 500
QVLGILKKSA LYIDVESDFH AKVPVVVCKD RKSALLCRVS AGNDMACLTT
510 520 530 540 550
DLLAALGKVE PVFTPLVLAF RYWAKLCYID SQTDGGIPSY CFALMVMFFL
560 570 580 590 600
QQRKPPLLPC LLGSWIEGFD PKRMDDFQLK GIVEEKFVKW EYNSSSATEK
610 620 630 640 650
NLIADENKAK ADEPKDDTKK TETDNQSNAA KAKHGKSPLT LEAPNQVPLG
660 670 680 690 700
QLWLELLKFY TLDFALEEYV ICVRIQDILT RENKNWPKRR IAIEDPFSVK
710 720 730 740 750
RNVARSLNSQ LVYEYVVERF RAAYRYFACP QKKGGNKSTM DPKKKEKGKL
760 770 780 790 800
SSKKPVKSDC SATNCCILGE SAEKIHMERG QPAKHDETEF TSQRCIVDND
810 820 830 840 850
SLLVNELGLA NHGQDSSSLS TASGGSDLKQ KSAEKQGDLT PSETSLKKEL
860 870 880 890 900
SQCICIGTPD GAESAGTDCR SNLEMDSSHQ IVCNNVSATS CNCKATEVTS
910 920 930 940 950
DLVDEDNLPS QELYYVFDKF ILTSGKPPTI VCSICKKDGH SKNDCPEDFR
960 970 980 990 1000
KIDLKPLPPM TNRFREILDL VCKRCFDELS PPCSEQHNRE QILIGLEKFI
1010 1020 1030 1040 1050
QKEYDEKARL CLFGSSKNGF GFRDSDLDIC MTLEGHENAE KLNCKEIIEN
1060 1070 1080 1090 1100
LAKILKRHPG LRNILPITTA KVPIVKFEHR RSGLEGDISL YNTLAQHNTR
1110 1120 1130 1140 1150
MLATYAAIDP RVQYLGYTMK VFAKRCDIGD ASRGSLSSYA YILMVLYFLQ
1160 1170 1180 1190 1200
QRKPPVIPVL QEIFDGKQIP QRMVDGWNAF FFDKTEELKK RLPSLGKNTE
1210 1220 1230 1240 1250
SLGELWLGLL RFYTEEFDFK EYVISIRQKK LLTTFEKQWT SKCIAIEDPF
1260 1270 1280 1290 1300
DLNHNLGAGV SRKMTNFIMK AFINGRKLFG TPFYPLIGRE AEYFFDSRVL
1310 1320 1330 1340 1350
TDGELAPNDR CCRVCGKIGH YMKDCPKRKR LKKKDSEEEK EGNEEEKDSR
1360 1370 1380 1390 1400
DLLDSRDLRC FICGDAGHVR RECPEVKMAR QRNSSVAAAQ LVRNLVNAQQ
1410 1420 1430 1440 1450
VAGSAQQQSD QSIRTRQSSE CSDSPSYSPQ PQPFPQNSPQ PSALPPPPSQ
1460 1470 1480 1490 1500
PGSQPKLGPP QQGGQPPHQV QMPLYNFPQS PPAHYSPMHS MGLLPMHPLQ
1510 1520 1530 1540 1550
IPAPSWPIHG PMLHSAPGST PSNIGLNDPS IIFAQPAARP MAIPSPSHDG
1560 1570 1580 1590 1600
HWPRTVAPNS LVNNGAVGNS EPRFRGLNPP IPWEHAPRHF PLVPASWPYG
1610 1620 1630 1640
LHQNFMHQGN PRFQPKPFYA QADRCATRRC RERCPHPPRG NVSE
Length:1,644
Mass (Da):184,650
Last modified:September 22, 2009 - v2
Checksum:i5EE701412E6157A0
GO

Sequence cautioni

The sequence CAM23506 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAM25329 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti815 – 8151D → A in AAI50792 (PubMed:15489334).Curated
Sequence conflicti815 – 8151D → A in BAD90397 (Ref. 3) Curated
Sequence conflicti1336 – 13361S → R in BAE22125 (PubMed:16141072).Curated
Sequence conflicti1422 – 14221S → C in BAE22125 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL626783, AL627238 Genomic DNA. Translation: CAM23506.1. Sequence problems.
AL627238, AL626783 Genomic DNA. Translation: CAM25329.1. Sequence problems.
BC150791 mRNA. Translation: AAI50792.1.
AK220327 mRNA. Translation: BAD90397.1.
AK134388 mRNA. Translation: BAE22125.1.
CCDSiCCDS18451.1.
RefSeqiNP_780681.2. NM_175472.3.
XP_006503045.1. XM_006502982.1.
UniGeneiMm.25181.
Mm.446602.

Genome annotation databases

EnsembliENSMUST00000043368; ENSMUSP00000044836; ENSMUSG00000034610.
GeneIDi230594.
KEGGimmu:230594.
UCSCiuc008ubd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL626783, AL627238 Genomic DNA. Translation: CAM23506.1. Sequence problems.
AL627238, AL626783 Genomic DNA. Translation: CAM25329.1. Sequence problems.
BC150791 mRNA. Translation: AAI50792.1.
AK220327 mRNA. Translation: BAD90397.1.
AK134388 mRNA. Translation: BAE22125.1.
CCDSiCCDS18451.1.
RefSeqiNP_780681.2. NM_175472.3.
XP_006503045.1. XM_006502982.1.
UniGeneiMm.25181.
Mm.446602.

3D structure databases

ProteinModelPortaliB2RX14.
SMRiB2RX14. Positions 381-725, 978-1286.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230978. 1 interaction.
DIPiDIP-48571N.
STRINGi10090.ENSMUSP00000044836.

PTM databases

iPTMnetiB2RX14.
PhosphoSiteiB2RX14.

Proteomic databases

EPDiB2RX14.
MaxQBiB2RX14.
PaxDbiB2RX14.
PRIDEiB2RX14.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043368; ENSMUSP00000044836; ENSMUSG00000034610.
GeneIDi230594.
KEGGimmu:230594.
UCSCiuc008ubd.1. mouse.

Organism-specific databases

CTDi23318.
MGIiMGI:2445126. Zcchc11.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2277. Eukaryota.
COG5260. LUCA.
GeneTreeiENSGT00550000074490.
HOGENOMiHOG000231134.
InParanoidiB2RX14.
KOiK13291.

Miscellaneous databases

ChiTaRSiZcchc11. mouse.
PROiB2RX14.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034610.
ExpressionAtlasiB2RX14. baseline and differential.
GenevisibleiB2RX14. MM.

Family and domain databases

Gene3Di4.10.60.10. 4 hits.
InterProiIPR002058. PAP_assoc.
IPR002934. Polymerase_NTP_transf_dom.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF03828. PAP_assoc. 2 hits.
PF00098. zf-CCHC. 2 hits.
[Graphical view]
SMARTiSM00343. ZnF_C2HC. 3 hits.
[Graphical view]
SUPFAMiSSF57756. SSF57756. 2 hits.
PROSITEiPS50158. ZF_CCHC. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTUT4_MOUSE
AccessioniPrimary (citable) accession number: B2RX14
Secondary accession number(s): A2A8R8, Q3UYT6, Q5DU43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 22, 2009
Last modified: September 7, 2016
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.