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B2RX12 (MRP3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Canalicular multispecific organic anion transporter 2
Alternative name(s):
ATP-binding cassette sub-family C member 3
Multidrug resistance-associated protein 3
Gene names
Name:Abcc3
Synonyms:Cmoat2, Mrp3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1523 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May act as an inducible transporter in the biliary and intestinal excretion of organic anions. Acts as an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes. Ref.1

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

Ontologies

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: B2RX12-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: B2RX12-2)

The sequence of this isoform differs from the canonical sequence as follows:
     862-862: Missing.
Isoform 3 (identifier: B2RX12-3)

The sequence of this isoform differs from the canonical sequence as follows:
     803-827: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15231523Canalicular multispecific organic anion transporter 2
PRO_0000356238

Regions

Topological domain1 – 3535Extracellular Potential
Transmembrane36 – 5621Helical; Name=1; Potential
Topological domain57 – 7519Cytoplasmic Potential
Transmembrane76 – 9621Helical; Name=2; Potential
Topological domain97 – 1026Extracellular Potential
Transmembrane103 – 12321Helical; Name=3; Potential
Topological domain124 – 1296Cytoplasmic Potential
Transmembrane130 – 15021Helical; Name=4; Potential
Topological domain151 – 17020Extracellular Potential
Transmembrane171 – 19121Helical; Name=5; Potential
Topological domain192 – 301110Cytoplasmic Potential
Transmembrane302 – 32423Helical; Name=6; Potential
Topological domain325 – 34521Extracellular Potential
Transmembrane346 – 36621Helical; Name=7; Potential
Topological domain367 – 41953Cytoplasmic Potential
Transmembrane420 – 44021Helical; Name=8; Potential
Topological domain4411Extracellular Potential
Transmembrane442 – 46221Helical; Name=9; Potential
Topological domain463 – 53573Cytoplasmic Potential
Transmembrane536 – 55621Helical; Name=10; Potential
Topological domain557 – 56711Extracellular Potential
Transmembrane568 – 58821Helical; Name=11; Potential
Topological domain589 – 967379Cytoplasmic Potential
Transmembrane968 – 98821Helical; Name=12; Potential
Topological domain989 – 101325Extracellular Potential
Transmembrane1014 – 103421Helical; Name=13; Potential
Topological domain1035 – 107137Cytoplasmic Potential
Transmembrane1072 – 109221Helical; Name=14; Potential
Topological domain1093 – 10964Extracellular Potential
Transmembrane1097 – 111721Helical; Name=15; Potential
Topological domain1118 – 119174Cytoplasmic Potential
Transmembrane1192 – 121221Helical; Name=16; Potential
Topological domain1213 – 12197Extracellular Potential
Transmembrane1220 – 124021Helical; Name=17; Potential
Topological domain1241 – 1523283Cytoplasmic Potential
Domain310 – 593284ABC transmembrane type-1 1
Domain626 – 850225ABC transporter 1
Domain967 – 1248282ABC transmembrane type-1 2
Domain1287 – 1519233ABC transporter 2
Nucleotide binding660 – 6678ATP 1 Potential
Nucleotide binding1319 – 13268ATP 2 Potential

Amino acid modifications

Glycosylation181N-linked (GlcNAc...) Potential
Glycosylation10021N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence803 – 82725Missing in isoform 3.
VSP_036011
Alternative sequence8621Missing in isoform 2.
VSP_036012

Experimental info

Sequence conflict811W → R in AAQ10530. Ref.2
Sequence conflict811W → R in AAQ10531. Ref.2
Sequence conflict2471D → N in BAE33375. Ref.3
Sequence conflict2691G → R in BAE28953. Ref.3
Sequence conflict2691G → R in CAI25949. Ref.4
Sequence conflict7421E → G in BAE33375. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: A65E6730D2C32001

FASTA1,523169,124
        10         20         30         40         50         60 
MDRLCGSGEL GSKFWDSNLS IYTNTPDLTP CFQNSLLAWV PCIYLWAALP CYLFYLRHHQ 

        70         80         90        100        110        120 
LGYIVLSWLS RLKTALGVLL WCVSWVDLFY SFHGLIHGSS PAPVFFVTPL VVGITMLLAT 

       130        140        150        160        170        180 
LLIQYERLRG VQSSGVLIIF WLLCVICAII PFRSKILSAL AEGKILDPFR FTTFYIYFAL 

       190        200        210        220        230        240 
VFCALILSCF KEKPPLFSPE NLDTNPCPEA SAGFFSRLSF WWFTRLAILG YRRPLEDRDL 

       250        260        270        280        290        300 
WSLSEEDCSH KVVQRLLEAW QKQQNQASGS QTATAEPKIP GEDAVLLKPR PKSKQPSFLR 

       310        320        330        340        350        360 
ALVRTFTSSL LMSACFNLIQ NLLGFVNPQL LSILIRFISD PTAPTWWGFL LAGLMFLSST 

       370        380        390        400        410        420 
MQTLILHQYY HCIFVMALRL RTAIIGVIYR KALVITNSVK RESTVGEMVN LMSVDAQRFM 

       430        440        450        460        470        480 
DVSPFINLLW SAPLQVILAI YFLWQILGPS ALAGVAVIVL LIPLNGAVSM KMKTYQVKQM 

       490        500        510        520        530        540 
KFKDSRIKLM SEILNGIKVL KLYAWEPSFL EQVKGIRQSE LQLLRKGAYL QAISTFIWIC 

       550        560        570        580        590        600 
TPFLVTLITL GVYVYVDESN VLDAEKAFVS LSLFNILKIP LNMLPQLISG LTQASVSLKR 

       610        620        630        640        650        660 
IQDFLNQNEL DPQCVERKTI SPGYAITIHN GTFTWAQDLP PTLHSLNIQI PKGALVAVVG 

       670        680        690        700        710        720 
PVGCGKSSLV SALLGEMEKL EGVVSVKGSV AYVPQQAWIQ NCTLQENVLF GQPMNPKRYQ 

       730        740        750        760        770        780 
QALETCALLA DLDVLPGGDQ TEIGEKGINL SGGQRQRVSL ARAVYSDANI FLLDDPLSAV 

       790        800        810        820        830        840 
DSHVAKHIFD QVIGPEGVLA GKTRVLVTHG ISFLPQTDFI IVLAGGQVSE MGHYSALLQH 

       850        860        870        880        890        900 
DGSFANFLRN YAPDEDQEDH EAALQNANEE VLLLEDTLST HTDLTDNEPA IYEVRKQFMR 

       910        920        930        940        950        960 
EMSSLSSEGE VQNRTMPKKH TNSLEKEALV TKTKETGALI KEEIAETGNV KLSVYWDYAK 

       970        980        990       1000       1010       1020 
SMGLCTTLSI CLLYGGQSAA AIGANVWLSA WSNDAEEHGQ QNKTSVRLGV YAALGILQGL 

      1030       1040       1050       1060       1070       1080 
LVMLSAFTMV VGAIQAARLL HEALLHNKIR SPQSFFDTTP SGRILNRFSK DIYVIDEVLA 

      1090       1100       1110       1120       1130       1140 
PTILMLLNSF FTSISTIMVI VASTPLFMVV VLPLAVLYGF VQRFYVATSR QLKRLESISR 

      1150       1160       1170       1180       1190       1200 
SPIFSHFSET VTGTSVIRAY GRIQDFKVLS DTKVDNNQKS SYPYIASNRW LGVHVEFVGN 

      1210       1220       1230       1240       1250       1260 
CVVLFAALFA VIGRNSLNPG LVGLSVSYAL QVTMALNWMI RMISDLESNI IAVERVKEYS 

      1270       1280       1290       1300       1310       1320 
KTKTEAPWVV ESNRAPEGWP TRGMVEFRNY SVRYRPGLEL VLKNVTVHVQ GGEKVGIVGR 

      1330       1340       1350       1360       1370       1380 
TGAGKSSMTL CLFRILEAAE GEIVIDGLNV AHIGLHDLRS QLTIIPQDPI LFSGTLRMNL 

      1390       1400       1410       1420       1430       1440 
DPFGRYSEED IWRALELSHL NTFVSSQPAG LDFQCAEGGD NLSVGQRQLV CLARALLRKS 

      1450       1460       1470       1480       1490       1500 
RVLVLDEATA AIDLETDDLI QGTIRTQFED CTVLTIAHRL NTIMDYNRVL VLDKGVVAEF 

      1510       1520 
DSPVNLIAAG GIFYGMAKDA GLA 

« Hide

Isoform 2 [UniParc].

Checksum: 1E6AAEC3E6E35D08
Show »

FASTA1,522169,053
Isoform 3 [UniParc].

Checksum: 51E3AF70EA8E26E1
Show »

FASTA1,498166,459

References

« Hide 'large scale' references
[1]"Analysis of the in vivo functions of Mrp3."
Belinsky M.G., Dawson P.A., Shchaveleva I., Bain L.J., Wang R., Ling V., Chen Z.-S., Grinberg A., Westphal H., Klein-Szanto A., Lerro A., Kruh G.D.
Mol. Pharmacol. 68:160-168(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
[2]Shimizu H., Ishikawa T., Yabuuchi H.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3).
Strain: BALB/c.
Tissue: Liver.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Liver.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-1523 (ISOFORM 2).
Strain: FVB/N.
Tissue: Brain and Liver.
[6]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY841885 mRNA. Translation: AAX39010.1.
AY795569 mRNA. Translation: AAX33774.1.
AF534127 mRNA. Translation: AAQ10530.1.
AF534128 mRNA. Translation: AAQ10531.1.
AK149551 mRNA. Translation: BAE28953.1.
AK155664 mRNA. Translation: BAE33375.1.
AL645965 Genomic DNA. Translation: CAI25949.1.
BC048825 mRNA. Translation: AAH48825.1.
BC150788 mRNA. Translation: AAI50789.1.
CCDSCCDS25254.1. [B2RX12-2]
RefSeqNP_083876.3. NM_029600.3.
UniGeneMm.23942.

3D structure databases

ProteinModelPortalB2RX12.
SMRB2RX12. Positions 285-852, 972-1516.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL2073683.

PTM databases

PhosphoSiteB2RX12.

Proteomic databases

MaxQBB2RX12.
PaxDbB2RX12.
PRIDEB2RX12.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021231; ENSMUSP00000021231; ENSMUSG00000020865.
GeneID76408.
KEGGmmu:76408.
UCSCuc007kym.1. mouse. [B2RX12-2]
uc007kyn.1. mouse. [B2RX12-1]
uc011ycu.1. mouse. [B2RX12-3]

Organism-specific databases

CTD8714.
MGIMGI:1923658. Abcc3.

Phylogenomic databases

eggNOGCOG1132.
GeneTreeENSGT00750000117309.
HOVERGENHBG108314.
InParanoidB2RX12.
KOK05667.
OrthoDBEOG7MWGW0.
TreeFamTF105199.

Gene expression databases

ArrayExpressB2RX12.
BgeeB2RX12.
GenevestigatorB2RX12.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR005292. Multidrug-R_assoc.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
TIGRFAMsTIGR00957. MRP_assoc_pro. 1 hit.
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio345093.
PROB2RX12.
SOURCESearch...

Entry information

Entry nameMRP3_MOUSE
AccessionPrimary (citable) accession number: B2RX12
Secondary accession number(s): Q3U1W8 expand/collapse secondary AC list , Q56PH0, Q59DK9, Q59DL0, Q5SUF4, Q80ZK8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: July 1, 2008
Last modified: July 9, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot