SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B2RUR8

- OTU7B_MOUSE

UniProt

B2RUR8 - OTU7B_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
OTU domain-containing protein 7B
Gene
Otud7b
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Negatively regulates mucosal immunity against infections. Mediates deubiquitination of EGFR. Has deubiquitinating activity toward 'Lys-11', 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. In vitro, has preference for 'Lys-11'-linked polyubiquitin chains; however such data are unsure in vivo. Hydrolyzes both linear and branched forms of polyubiquitin.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

Deubiquitinase activity is inhibited following interactin with PARK7 By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei191 – 1911 By similarity
Active sitei194 – 1941Nucleophile Inferred
Active sitei358 – 3581Proton acceptor Inferred

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri793 – 82836A20-type
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. K63-linked polyubiquitin binding Source: BHF-UCL
  3. cysteine-type peptidase activity Source: UniProtKB
  4. protein binding Source: UniProtKB
  5. ubiquitin thiolesterase activity Source: UniProtKB
  6. ubiquitin-specific protease activity Source: UniProtKB
  7. zinc ion binding Source: InterPro

GO - Biological processi

  1. mucosal immune response Source: UniProtKB
  2. negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  3. negative regulation of transcription from RNA polymerase II promoter Source: ParkinsonsUK-UCL
  4. protein K11-linked deubiquitination Source: UniProtKB
  5. protein K48-linked deubiquitination Source: UniProtKB
  6. protein K63-linked deubiquitination Source: UniProtKB
  7. protein deubiquitination Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Immunity, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC64.001.

Names & Taxonomyi

Protein namesi
Recommended name:
OTU domain-containing protein 7B (EC:3.4.19.12)
Alternative name(s):
Cellular zinc finger anti-NF-kappa-B protein
Zinc finger A20 domain-containing protein 1
Zinc finger protein Cezanne
Gene namesi
Name:Otud7b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:2654703. Otud7b.

Subcellular locationi

Cytoplasm. Nucleus
Note: Shuttles be cytoplasm and the nucleus in a XPO1/CRM1-dependent manner.

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. microtubule cytoskeleton Source: Ensembl
  3. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice do not show obvious defects in survival, except a moderately reduced body weight. They however display hyperactivation of non-canonical NF-kappa-B without affecting canonical NF-kappa-B activation. Mice show B-cell hyper-responsiveness to antigens, lymphoid follicular hyperplasia in the intestinal mucosa and elevated host-defense ability against an intestinal bacterial pathogen, Citrobacter rodentium.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi194 – 1941C → S: Loss of deubiquitinating activity; when associated with R-358. 1 Publication
Mutagenesisi358 – 3581H → R: Loss of deubiquitinating activity; when associated with S-194. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 840840OTU domain-containing protein 7B
PRO_0000421819Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei100 – 1001Phosphoserine By similarity

Post-translational modificationi

Phosphorylated by EGFR By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiB2RUR8.
PaxDbiB2RUR8.
PRIDEiB2RUR8.

Expressioni

Gene expression databases

ArrayExpressiB2RUR8.
GenevestigatoriB2RUR8.

Interactioni

Subunit structurei

Interacts with TRAF6. Interacts with PARK7, leading to inhibit deubiquitinase activity. Interacts with EGFR, ITCH and NEDD4 By similarity. Interacts with TRAF3.1 Publication

Protein-protein interaction databases

DIPiDIP-60128N.
IntActiB2RUR8. 1 interaction.
STRINGi10090.ENSMUSP00000088291.

Structurei

3D structure databases

ProteinModelPortaliB2RUR8.
SMRiB2RUR8. Positions 1-59, 147-395.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini183 – 365183OTU
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni152 – 401250TRAF-binding By similarity
Add
BLAST
Regioni167 – 440274Catalytic By similarity
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi483 – 49816Nuclear localization signal Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C64 family.
Contains 1 OTU domain.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG282016.
GeneTreeiENSGT00530000062989.
HOGENOMiHOG000048103.
HOVERGENiHBG050904.
InParanoidiB2RUR8.
KOiK11860.
OMAiYATFPRQ.
OrthoDBiEOG7JHM57.
PhylomeDBiB2RUR8.
TreeFamiTF323312.

Family and domain databases

InterProiIPR003323. OTU.
IPR002653. Znf_A20.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
PS51036. ZF_A20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2RUR8-1 [UniParc]FASTAAdd to Basket

« Hide

MTLDMDAVLS DFVRSTGAEP GLARDLLEGK NWDVSAALSD FEQLRQVHAG    50
NLSPPFSGGS TCPKTPEKGG SDREPTRPSR PILQRQDDVI QEKRLSRGIS 100
HASSSIVSLA RSHVSSNGGG GGSSEHPLEM PICAFQLPDL TVYKEDFRSF 150
IERDLIEQSM LVALEQAGRL NWWVSMDSTC QRLLPLATTG DGNCLLHAAS 200
LGMWGFHDRD LVLRKALYAL MEKGVEKEAL RRRWRWQQTQ QNKESGLVYT 250
EDEWQKEWNE LIKLASSEPR MHLGSNGASG GGVESSEEPV YESLEEFHVF 300
VLAHVLKRPI VVVADTMLRD SGGEAFAPIP FGGIYLPLEV PASQCHRSPL 350
VLAYDQAHFS ALVSMEQKES AKEQAVIPLT DSEHKLLPLH FAVDPGKGWE 400
WGKDDNDNVR LASIILSLEV KLHLLHSYMN VKWIPLSSDS QAPLAQPESP 450
TASAGDEPRS TPESGESDKE SVGSSSLGNE GSRRKEKSKR DREKDKKRAD 500
SVANKLGSFG KTLGSKLKKN MGGLMHSKGP KPGGLGSGSG ISSGTETLEK 550
KKKNNTLKSW KGGKEEAAGD GPVSEKPPSE SVGNGGSKYS QEVMQSLSTM 600
RIAMQGEGKY IFVGTLKMGH RHQYQEEMIQ RYLADAEERF LAEQKQKEVE 650
RKIMNGGLVS GPPPAKKPEP DGGEDQPSDS PAEPKAMAFS TAYPGGFTIP 700
RPSGGGVHCQ EPRRQLAGGP CVGGLPSYAT FPRQYPGRPY PHQDNIPALE 750
PGKDGVHRGA LLPPQFRVAD SYSNGYREPP EPDGWAGAPR GLPPTQTKCK 800
QPNCSFYGHP ETNNLCSCCY REELRRRERE PGGELLAHRF 840
Length:840
Mass (Da):91,983
Last modified:July 1, 2008 - v1
Checksum:iB63C06B656CC02EE
GO

Sequence cautioni

The sequence AAH37040.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC092094 Genomic DNA. No translation available.
CH466620 Genomic DNA. Translation: EDL38862.1.
BC141397 mRNA. Translation: AAI41398.1.
BC037040 mRNA. Translation: AAH37040.1. Sequence problems.
BC141398 mRNA. Translation: AAI41399.1.
CCDSiCCDS17629.1.
RefSeqiNP_001020784.1. NM_001025613.1.
NP_001020785.1. NM_001025614.1.
XP_006501447.1. XM_006501384.1.
XP_006501448.1. XM_006501385.1.
XP_006501449.1. XM_006501386.1.
XP_006501450.1. XM_006501387.1.
XP_006501451.1. XM_006501388.1.
XP_006501452.1. XM_006501389.1.
UniGeneiMm.272336.
Mm.487453.

Genome annotation databases

EnsembliENSMUST00000035519; ENSMUSP00000046413; ENSMUSG00000038495.
ENSMUST00000090785; ENSMUSP00000088291; ENSMUSG00000038495.
ENSMUST00000098849; ENSMUSP00000096449; ENSMUSG00000038495.
GeneIDi229603.
KEGGimmu:229603.
UCSCiuc008qma.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC092094 Genomic DNA. No translation available.
CH466620 Genomic DNA. Translation: EDL38862.1 .
BC141397 mRNA. Translation: AAI41398.1 .
BC037040 mRNA. Translation: AAH37040.1 . Sequence problems.
BC141398 mRNA. Translation: AAI41399.1 .
CCDSi CCDS17629.1.
RefSeqi NP_001020784.1. NM_001025613.1.
NP_001020785.1. NM_001025614.1.
XP_006501447.1. XM_006501384.1.
XP_006501448.1. XM_006501385.1.
XP_006501449.1. XM_006501386.1.
XP_006501450.1. XM_006501387.1.
XP_006501451.1. XM_006501388.1.
XP_006501452.1. XM_006501389.1.
UniGenei Mm.272336.
Mm.487453.

3D structure databases

ProteinModelPortali B2RUR8.
SMRi B2RUR8. Positions 1-59, 147-395.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60128N.
IntActi B2RUR8. 1 interaction.
STRINGi 10090.ENSMUSP00000088291.

Protein family/group databases

MEROPSi C64.001.

Proteomic databases

MaxQBi B2RUR8.
PaxDbi B2RUR8.
PRIDEi B2RUR8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000035519 ; ENSMUSP00000046413 ; ENSMUSG00000038495 .
ENSMUST00000090785 ; ENSMUSP00000088291 ; ENSMUSG00000038495 .
ENSMUST00000098849 ; ENSMUSP00000096449 ; ENSMUSG00000038495 .
GeneIDi 229603.
KEGGi mmu:229603.
UCSCi uc008qma.1. mouse.

Organism-specific databases

CTDi 56957.
MGIi MGI:2654703. Otud7b.

Phylogenomic databases

eggNOGi NOG282016.
GeneTreei ENSGT00530000062989.
HOGENOMi HOG000048103.
HOVERGENi HBG050904.
InParanoidi B2RUR8.
KOi K11860.
OMAi YATFPRQ.
OrthoDBi EOG7JHM57.
PhylomeDBi B2RUR8.
TreeFami TF323312.

Miscellaneous databases

NextBioi 379553.
PROi B2RUR8.
SOURCEi Search...

Gene expression databases

ArrayExpressi B2RUR8.
Genevestigatori B2RUR8.

Family and domain databases

InterProi IPR003323. OTU.
IPR002653. Znf_A20.
[Graphical view ]
Pfami PF02338. OTU. 1 hit.
[Graphical view ]
PROSITEi PS50802. OTU. 1 hit.
PS51036. ZF_A20. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Eye.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "OTUD7B controls non-canonical NF-kappaB activation through deubiquitination of TRAF3."
    Hu H., Brittain G.C., Chang J.H., Puebla-Osorio N., Jin J., Zal A., Xiao Y., Cheng X., Chang M., Fu Y.X., Zal T., Zhu C., Sun S.C.
    Nature 494:371-374(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH TRAF3, MUTAGENESIS OF CYS-194 AND HIS-358.

Entry informationi

Entry nameiOTU7B_MOUSE
AccessioniPrimary (citable) accession number: B2RUR8
Secondary accession number(s): Q8CFS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: July 1, 2008
Last modified: September 3, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi