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Protein

Amyloid beta A4 precursor protein-binding family A member 1

Gene

Apba1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-AAP (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • chemical synaptic transmission Source: MGI
  • gamma-aminobutyric acid secretion Source: MGI
  • glutamate secretion Source: MGI
  • intracellular protein transport Source: MGI
  • in utero embryonic development Source: MGI
  • locomotory behavior Source: MGI
  • multicellular organism growth Source: MGI
  • protein complex assembly Source: InterPro
  • regulation of gene expression Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-212676. Dopamine Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Amyloid beta A4 precursor protein-binding family A member 1
Alternative name(s):
Adapter protein X11alpha
Neuron-specific X11 protein
Neuronal Munc18-1-interacting protein 1
Short name:
Mint-1
Gene namesi
Name:Apba1
Synonyms:Mint1, X11
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1860297. Apba1.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: UniProtKB-SubCell
  • membrane Source: MGI
  • nucleus Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
  • synaptic vesicle Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004175011 – 842Amyloid beta A4 precursor protein-binding family A member 1Add BLAST842

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei82PhosphoserineCombined sources1
Modified residuei246PhosphoserineCombined sources1
Modified residuei250PhosphoserineCombined sources1
Modified residuei252PhosphoserineCombined sources1
Modified residuei267PhosphoserineCombined sources1
Modified residuei284PhosphoserineCombined sources1
Modified residuei289PhosphoserineBy similarity1
Modified residuei309PhosphothreonineCombined sources1
Modified residuei317PhosphoserineCombined sources1
Modified residuei372PhosphoserineCombined sources1
Modified residuei375PhosphothreonineCombined sources1
Modified residuei406PhosphoserineCombined sources1
Modified residuei408PhosphoserineCombined sources1
Modified residuei413PhosphoserineCombined sources1
Modified residuei573PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiB2RUJ5.
PeptideAtlasiB2RUJ5.
PRIDEiB2RUJ5.

PTM databases

iPTMnetiB2RUJ5.
PhosphoSitePlusiB2RUJ5.

Expressioni

Tissue specificityi

Isoform 3 is expressed in brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000024897.
GenevisibleiB2RUJ5. MM.

Interactioni

Subunit structurei

Part of a multimeric complex containing Munc18-1 and syntaxin-1. Also part of the brain-specific heterotrimeric complex LIN-10/X11-alpha, LIN-2/CASK, and LIN7. Binds to the cytoplasmic domain of amyloid protein (APP) (By similarity). Interacts (via PDZ 1 and 2 domains) with FSPB (By similarity). Isoform 3 interacts (via its truncated PID domain) with active, GTP-bound RAB6A. Also interacts with GTP-bound RAB6B (By similarity).By similarity

Protein-protein interaction databases

BioGridi235626. 3 interactors.
IntActiB2RUJ5. 2 interactors.
STRINGi10090.ENSMUSP00000025830.

Structurei

3D structure databases

ProteinModelPortaliB2RUJ5.
SMRiB2RUJ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini460 – 648PIDPROSITE-ProRule annotationAdd BLAST189
Domaini661 – 746PDZ 1PROSITE-ProRule annotationAdd BLAST86
Domaini752 – 828PDZ 2PROSITE-ProRule annotationAdd BLAST77

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni631 – 648Autoinhibitory helix linkerBy similarityAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi40 – 67Pro-richAdd BLAST28
Compositional biasi298 – 334Pro-richAdd BLAST37
Compositional biasi373 – 457Pro-richAdd BLAST85

Domaini

Composed of an N-terminal domain that binds Munc18-1 and LIN-2/CASK, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.By similarity
The autoinhibitory helix linker occludes the APP binding site.By similarity
The PID domain, truncated by 11 amino acids, as observed in isoform 3, but not full-length, mediates the interaction with RAB6A.By similarity

Sequence similaritiesi

Contains 2 PDZ (DHR) domains.PROSITE-ProRule annotation
Contains 1 PID domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3605. Eukaryota.
ENOG410XSBD. LUCA.
GeneTreeiENSGT00390000013578.
HOVERGENiHBG050523.
InParanoidiB2RUJ5.
KOiK04531.
OMAiPNHLHFH.
OrthoDBiEOG091G0PDJ.
PhylomeDBiB2RUJ5.
TreeFamiTF315245.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
2.30.42.10. 2 hits.
InterProiIPR030530. Apba1.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
[Graphical view]
PANTHERiPTHR12345:SF14. PTHR12345:SF14. 1 hit.
PfamiPF00595. PDZ. 2 hits.
PF00640. PID. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 2 hits.
SM00462. PTB. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50106. PDZ. 2 hits.
PS01179. PID. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: B2RUJ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNHLEGSAEV EVADEAPGGE VNESVEADLE HPEVVEGQQP SPSPPPPAGH
60 70 80 90 100
EPEDHRGHPA PPPPPPPQEE EEEERGECLA RSASTESGFH NHTDTAEGDV
110 120 130 140 150
LAAARDGYEA ERAQDADDES AYAVQYRPEA EEYTEQAEAE HVEAAQRRAL
160 170 180 190 200
PNHLHFHSLE HEEAMNAAYS GYVYTHRLFH RAEDEPYAEP YADYGGLQEH
210 220 230 240 250
VYEEIGDAPE LEARDGLRLY ERERDEAAAY RQEALGARLH HYDERSDGES
260 270 280 290 300
DSPEKEAEFA PYPRMDSYEQ EEDIDQIVAE VKQSMSSQSL DKAAEDMPEA
310 320 330 340 350
EQDLERAPTP GGGHPDSPGL PAPAGQQQRV VGTPGGSEVG QRYSKEKRDA
360 370 380 390 400
ISLAIKDIKE AIEEVKTRTI RSPYTPDEPK EPIWVMRQDI SPTRDCDDQR
410 420 430 440 450
PVDGDSPSPG SSSPLGAESS SIPLHPGDPT EASTNKESRK SLASFPTYVE
460 470 480 490 500
VPGPCDPEDL IDGIIFAANY LGSTQLLSDK TPSKNVRMMQ AQEAVSRIKT
510 520 530 540 550
AQKLAKSRKK APEGESQPMT EVDLFISTQR IKVLNADTQE PMMDHPLRTI
560 570 580 590 600
SYIADIGNIV VLMARRRMPR SNSQENVEAS HPSQDGKRQY KMICHVFESE
610 620 630 640 650
DAQLIAQSIG QAFSVAYQEF LRANGINPED LSQKEYSDLL NTQDMYNDDL
660 670 680 690 700
IHFSKSENCK DVFIEKQKGE ILGVVIVESG WGSILPTVII ANMMHGGPAE
710 720 730 740 750
KSGKLNIGDQ IMSINGTSLV GLPLSTCQSI IKGLKNQSRV KLNIVRCPPV
760 770 780 790 800
TTVLIRRPDL RYQLGFSVQN GIICSLMRGG IAERGGVRVG HRIIEINGQS
810 820 830 840
VVATPHEKIV HILSNAVGEI HMKTMPAAMY RLLTAQEQPV YI
Length:842
Mass (Da):92,909
Last modified:May 16, 2012 - v2
Checksum:iE00B754EDCF1B1D4
GO
Isoform 2 (identifier: B2RUJ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-390: Missing.
     391-405: SPTRDCDDQRPVDGD → MENFWMYQFESHEEE

Show »
Length:452
Mass (Da):49,936
Checksum:i2C9FA41FD7FCF340
GO
Isoform 3 (identifier: B2RUJ5-3) [UniParc]FASTAAdd to basket
Also known as: Mint1_826

The sequence of this isoform differs from the canonical sequence as follows:
     500-510: Missing.

Note: This isoform interacts with RAB6 GTPases.
Show »
Length:831
Mass (Da):91,669
Checksum:i170DDCF2204F0C86
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti142V → A in AAI41182 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0437631 – 390Missing in isoform 2. 1 PublicationAdd BLAST390
Alternative sequenceiVSP_043764391 – 405SPTRD…PVDGD → MENFWMYQFESHEEE in isoform 2. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_053519500 – 510Missing in isoform 3. CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032261 mRNA. Translation: BAC27784.1.
AK147583 mRNA. Translation: BAE28008.1.
AC134830 Genomic DNA. No translation available.
AC148021 Genomic DNA. No translation available.
CH466534 Genomic DNA. Translation: EDL41607.1.
CH466534 Genomic DNA. Translation: EDL41608.1.
BC141181 mRNA. Translation: AAI41182.1.
CCDSiCCDS50407.1. [B2RUJ5-1]
RefSeqiNP_796008.2. NM_177034.3. [B2RUJ5-1]
XP_006527202.1. XM_006527139.3. [B2RUJ5-1]
XP_006527203.1. XM_006527140.3. [B2RUJ5-3]
UniGeneiMm.22879.

Genome annotation databases

EnsembliENSMUST00000025830; ENSMUSP00000025830; ENSMUSG00000024897. [B2RUJ5-1]
GeneIDi319924.
KEGGimmu:319924.
UCSCiuc008hai.1. mouse. [B2RUJ5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032261 mRNA. Translation: BAC27784.1.
AK147583 mRNA. Translation: BAE28008.1.
AC134830 Genomic DNA. No translation available.
AC148021 Genomic DNA. No translation available.
CH466534 Genomic DNA. Translation: EDL41607.1.
CH466534 Genomic DNA. Translation: EDL41608.1.
BC141181 mRNA. Translation: AAI41182.1.
CCDSiCCDS50407.1. [B2RUJ5-1]
RefSeqiNP_796008.2. NM_177034.3. [B2RUJ5-1]
XP_006527202.1. XM_006527139.3. [B2RUJ5-1]
XP_006527203.1. XM_006527140.3. [B2RUJ5-3]
UniGeneiMm.22879.

3D structure databases

ProteinModelPortaliB2RUJ5.
SMRiB2RUJ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi235626. 3 interactors.
IntActiB2RUJ5. 2 interactors.
STRINGi10090.ENSMUSP00000025830.

PTM databases

iPTMnetiB2RUJ5.
PhosphoSitePlusiB2RUJ5.

Proteomic databases

PaxDbiB2RUJ5.
PeptideAtlasiB2RUJ5.
PRIDEiB2RUJ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025830; ENSMUSP00000025830; ENSMUSG00000024897. [B2RUJ5-1]
GeneIDi319924.
KEGGimmu:319924.
UCSCiuc008hai.1. mouse. [B2RUJ5-1]

Organism-specific databases

CTDi320.
MGIiMGI:1860297. Apba1.

Phylogenomic databases

eggNOGiKOG3605. Eukaryota.
ENOG410XSBD. LUCA.
GeneTreeiENSGT00390000013578.
HOVERGENiHBG050523.
InParanoidiB2RUJ5.
KOiK04531.
OMAiPNHLHFH.
OrthoDBiEOG091G0PDJ.
PhylomeDBiB2RUJ5.
TreeFamiTF315245.

Enzyme and pathway databases

ReactomeiR-MMU-212676. Dopamine Neurotransmitter Release Cycle.

Miscellaneous databases

PROiB2RUJ5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024897.
GenevisibleiB2RUJ5. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
2.30.42.10. 2 hits.
InterProiIPR030530. Apba1.
IPR001478. PDZ.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
[Graphical view]
PANTHERiPTHR12345:SF14. PTHR12345:SF14. 1 hit.
PfamiPF00595. PDZ. 2 hits.
PF00640. PID. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 2 hits.
SM00462. PTB. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50106. PDZ. 2 hits.
PS01179. PID. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPBA1_MOUSE
AccessioniPrimary (citable) accession number: B2RUJ5
Secondary accession number(s): Q3UH49, Q8BMF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: May 16, 2012
Last modified: November 2, 2016
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.