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Protein

RNA polymerase I-specific transcription initiation factor RRN3

Gene

Rrn3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction.2 Publications

GO - Molecular functioni

GO - Biological processi

  • cell proliferation Source: MGI
  • cytoplasm organization Source: MGI
  • DNA-templated transcription, initiation Source: MGI
  • homeostasis of number of cells Source: MGI
  • in utero embryonic development Source: MGI
  • negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator Source: MGI
  • nucleolus organization Source: MGI
  • positive regulation of neuron projection development Source: Ensembl
  • positive regulation of transcription, DNA-templated Source: MGI
  • regulation of DNA-templated transcription, initiation Source: MGI
  • ribosome biogenesis Source: MGI
  • transcription initiation from RNA polymerase I promoter Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-73762. RNA Polymerase I Transcription Initiation.
R-MMU-73772. RNA Polymerase I Promoter Escape.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase I-specific transcription initiation factor RRN3
Gene namesi
Name:Rrn3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1925255. Rrn3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 656656RNA polymerase I-specific transcription initiation factor RRN3PRO_0000384905Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei168 – 1681PhosphoserineCombined sources
Modified residuei170 – 1701PhosphoserineCombined sources
Modified residuei198 – 1981Phosphothreonine; by MAPK9By similarity
Modified residuei638 – 6381PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated at Thr-198 by MAPK9/JNK2, which abrogates initiation complex formation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiB2RS91.
MaxQBiB2RS91.
PaxDbiB2RS91.
PRIDEiB2RS91.

PTM databases

iPTMnetiB2RS91.
PhosphoSiteiB2RS91.

Expressioni

Gene expression databases

BgeeiB2RS91.
GenevisibleiB2RS91. MM.

Interactioni

Subunit structurei

Interacts with TAF1B. Interacts with TWISTNB, EIF3L and TAF1C (By similarity).By similarity

GO - Molecular functioni

  • RNA polymerase binding Source: MGI

Protein-protein interaction databases

BioGridi223022. 3 interactions.
STRINGi10090.ENSMUSP00000023363.

Structurei

3D structure databases

ProteinModelPortaliB2RS91.
SMRiB2RS91. Positions 54-583.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni498 – 650153Interaction with TWISTNBBy similarityAdd
BLAST
Regioni555 – 65096Interaction with EIF3LBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the RRN3 family.Curated

Phylogenomic databases

eggNOGiKOG2434. Eukaryota.
ENOG410XRJJ. LUCA.
GeneTreeiENSGT00390000001488.
HOGENOMiHOG000236344.
InParanoidiB2RS91.
KOiK15216.
OMAiIQVDMED.
OrthoDBiEOG7ZGX2R.
PhylomeDBiB2RS91.
TreeFamiTF312979.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR007991. RNA_pol_I_trans_ini_fac_RRN3.
[Graphical view]
PANTHERiPTHR12790. PTHR12790. 1 hit.
PfamiPF05327. RRN3. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.

Sequencei

Sequence statusi: Complete.

B2RS91-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPLLHTRL SGDVTAAASA TLSASRTGLS DMLALESDFF NSPPKKTVRF
60 70 80 90 100
GGTVTEVLLK YKKGETNDLE LLKNQLSDPD IKDDQIINWL LEFRSSVMYL
110 120 130 140 150
TKDFEQLINI ILRLPWLNRS QRVVEEYLAF LGNLVSAQTV FLRPCLSMIA
160 170 180 190 200
SHFVPPRVIV KEGGIDVSDS DDEDDNLPAI FDTCHRALQI ITRYVPSTPW
210 220 230 240 250
FLMPILVEKF PFVRKSERTL ECYVHNLLRI SLYFPTLRRE ILELVIEKLL
260 270 280 290 300
KLDVSVSRQD IEDAEEKAAQ TCGGTDTTEG LFNMDEDEDT DPEKKADQEQ
310 320 330 340 350
PNQMAHPTAE RLDVLLCLLL SYIEDVCRVH GKIDNNKTKD LYRDLISIFD
360 370 380 390 400
KLLLPTHASC HVQFFMFFLC SFKLGFAEAF LEHLWKKLQD PNNPAIIRQA
410 420 430 440 450
AANYIGSFLA RAKFIPLITV KTCLDLLVNW LHMYLTNQDS GTKAFCDVAL
460 470 480 490 500
HGPFYSACQA VFYTVVFRHK QLLSGNLKQG LQYLQSLNFE RIVLSQLNPL
510 520 530 540 550
KICLPQVVNF FAAITNKYQL VFCYTIMERN SRQMLPVIRS TAGGDSVQTC
560 570 580 590 600
TNPLDTFFPF DPCVLKRSKK FIDPIYQIWE DGSAEELQEF KKSTKKEVVE
610 620 630 640 650
DEDDDFLKGE VPQSDTVTGL TPSSFDTHFQ SPSSSVGSPP VLYIPGQSPL

LTRIYD
Length:656
Mass (Da):74,518
Last modified:July 1, 2008 - v1
Checksum:i8C83C4893E2C2257
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti597 – 5971E → Q in AAH55781 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC055781 mRNA. Translation: AAH55781.1.
BC034110 mRNA. Translation: AAH34110.1.
BC138774 mRNA. Translation: AAI38775.1.
BC138776 mRNA. Translation: AAI38777.1.
CCDSiCCDS27968.1.
RefSeqiNP_001034610.1. NM_001039521.1.
UniGeneiMm.288606.
Mm.490362.

Genome annotation databases

EnsembliENSMUST00000023363; ENSMUSP00000023363; ENSMUSG00000022682.
GeneIDi106298.
KEGGimmu:106298.
UCSCiuc007ygj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC055781 mRNA. Translation: AAH55781.1.
BC034110 mRNA. Translation: AAH34110.1.
BC138774 mRNA. Translation: AAI38775.1.
BC138776 mRNA. Translation: AAI38777.1.
CCDSiCCDS27968.1.
RefSeqiNP_001034610.1. NM_001039521.1.
UniGeneiMm.288606.
Mm.490362.

3D structure databases

ProteinModelPortaliB2RS91.
SMRiB2RS91. Positions 54-583.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi223022. 3 interactions.
STRINGi10090.ENSMUSP00000023363.

PTM databases

iPTMnetiB2RS91.
PhosphoSiteiB2RS91.

Proteomic databases

EPDiB2RS91.
MaxQBiB2RS91.
PaxDbiB2RS91.
PRIDEiB2RS91.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023363; ENSMUSP00000023363; ENSMUSG00000022682.
GeneIDi106298.
KEGGimmu:106298.
UCSCiuc007ygj.1. mouse.

Organism-specific databases

CTDi54700.
MGIiMGI:1925255. Rrn3.

Phylogenomic databases

eggNOGiKOG2434. Eukaryota.
ENOG410XRJJ. LUCA.
GeneTreeiENSGT00390000001488.
HOGENOMiHOG000236344.
InParanoidiB2RS91.
KOiK15216.
OMAiIQVDMED.
OrthoDBiEOG7ZGX2R.
PhylomeDBiB2RS91.
TreeFamiTF312979.

Enzyme and pathway databases

ReactomeiR-MMU-73762. RNA Polymerase I Transcription Initiation.
R-MMU-73772. RNA Polymerase I Promoter Escape.

Miscellaneous databases

ChiTaRSiRrn3. mouse.
PROiB2RS91.
SOURCEiSearch...

Gene expression databases

BgeeiB2RS91.
GenevisibleiB2RS91. MM.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR007991. RNA_pol_I_trans_ini_fac_RRN3.
[Graphical view]
PANTHERiPTHR12790. PTHR12790. 1 hit.
PfamiPF05327. RRN3. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain, Eye and Lung.
  2. "Rrn3 phosphorylation is a regulatory checkpoint for ribosome biogenesis."
    Cavanaugh A.H., Hirschler-Laszkiewicz I., Hu Q., Dundr M., Smink T., Misteli T., Rothblum L.I.
    J. Biol. Chem. 277:27423-27432(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH TAF1B.
  3. "Rrn3 becomes inactivated in the process of ribosomal DNA transcription."
    Hirschler-Laszkiewicz I., Cavanaugh A.H., Mirza A., Lun M., Hu Q., Smink T., Rothblum L.I.
    J. Biol. Chem. 278:18953-18959(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168 AND SER-170, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Lung, Spleen and Testis.

Entry informationi

Entry nameiRRN3_MOUSE
AccessioniPrimary (citable) accession number: B2RS91
Secondary accession number(s): Q7TNE7, Q8K052
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 1, 2008
Last modified: June 8, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.