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B2RRE7 (OTUD4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
OTU domain-containing protein 4

EC=3.4.19.12
Gene names
Name:Otud4
Synonyms:Kiaa1046
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1107 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Deubiquitinating enzyme that specifically hydrolyzes 'Lys-48'-linked polyubiquitin By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sequence similarities

Contains 1 OTU domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11071107OTU domain-containing protein 4
PRO_0000394458

Regions

Domain34 – 155122OTU
Region39 – 457Cys-loop By similarity
Region94 – 10411Variable-loop By similarity
Region143 – 1486His-loop By similarity

Sites

Active site421 By similarity
Active site451Nucleophile By similarity
Active site1481 By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity UniProtKB Q01804
Modified residue4381Phosphotyrosine Ref.4
Modified residue4421Phosphoserine By similarity UniProtKB Q01804
Modified residue4591Phosphotyrosine By similarity
Modified residue8951Phosphoserine By similarity
Modified residue10001Phosphoserine Ref.5
Modified residue10051Phosphoserine Ref.5
Modified residue10161Phosphoserine Ref.5 Ref.6 PubMed 18973353
Modified residue10171Phosphoserine Ref.5 Ref.6 PubMed 18973353

Experimental info

Sequence conflict2901Missing in AAI45260. Ref.1

Sequences

Sequence LengthMass (Da)Tools
B2RRE7 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: 746B6BC8589FA672

FASTA1,107123,055
        10         20         30         40         50         60 
MEAAVGAPDG VDQGGVGPLE DETPMDAYLR KLGLYRKLVA KDGSCLFRAV AEQVLHSQSR 

        70         80         90        100        110        120 
HVEVRMACIR YLRENREKFE AFIEGSFEEY LKRLENPQEW VGQVEISALS LMYRKDFVIY 

       130        140        150        160        170        180 
QEPNVSPSHV TENNFPEKVL LCFSNGNHYD IVYPITYKDS SAMCQSLLYE LLYEKVFKTD 

       190        200        210        220        230        240 
VSKIMMGLEA SEVAEESNSE ISDSEDDSCK SKSTAATDVN GFKPSGSENP KNNGNSADLP 

       250        260        270        280        290        300 
LSRKVLKSLN PAVYRNVEYE IWLKSKQAQQ KRDYSIAAGL QYEVGDKCHQ VRLDHNGKLS 

       310        320        330        340        350        360 
NADIHGVHSE NGLVLSEELG KKHTPKNLKP PPPESWNTVS GKKMKKPNSG QNFHSDTDYR 

       370        380        390        400        410        420 
GPKNLNKPIK APSALPPRLQ HPSSGVRQHA FSSHSTGSQS QKSSSEHKNL SRMPSQITRK 

       430        440        450        460        470        480 
PDRERAEDFD HVSRESYYFG LSPEERREKQ AIEESRLLYE IQNRDEQAFP ALSSSSVSQS 

       490        500        510        520        530        540 
PSQNSNACVP RKSSHARDRK GSMRRADAEE RKDKDSLRGH THVDKKPEPS TLEISDDKCT 

       550        560        570        580        590        600 
RVSSPSKSKK ECPSPVEQKP AEHIPLSNPA PLLVSPEVHL TPAVPSLPAT VPAWPSEPTT 

       610        620        630        640        650        660 
FGPTGVPAQI PILSVTQTTG PDAAVSQAHL TPSPVPVSIQ AVNQPLMPLP QTMSLYQDPL 

       670        680        690        700        710        720 
YPGFPCSEKG DRAIAPPYSL CQTGEDLPKD KNILRFFFNL GVKAYSCPMW APHSYLYPLH 

       730        740        750        760        770        780 
QAYMAACRMY PKVPVPVYPQ NTWFQEAPPA QSESDCPCTD AHYSLHPEAS VNGQMPQAEM 

       790        800        810        820        830        840 
GPPAFASPLV IPPSQVSEGH GQLSYQPELE SENPGQLLHA EYEESLSGKN MYPQQSFGPN 

       850        860        870        880        890        900 
PFLGPVPIAP PFFPHVWYGY PFQGFVENPV MRQNIVLPPD DKGELDLPLE NLDLSKECDS 

       910        920        930        940        950        960 
VSAVDEFPDA RVEGAHSLSA ASVSSKHEGR VEQSSQTRKA DIDLASGSSA VEGKGHPPTQ 

       970        980        990       1000       1010       1020 
ILNREREPGS AEPEPKRTIQ SLKEKPEKVK DPKTAADVVS PGANSVDRLQ RPKEESSEDE 

      1030       1040       1050       1060       1070       1080 
NEVSNILRSG RSKQFYNQTY GSRKYKSDWG SSGRGGYQHV RGEESWKGQP NRSRDEGYQY 

      1090       1100 
HRHVRGRPYR GDRRRSGMGD GHRGQHT 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[2]"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 161-1107.
Tissue: Brain.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 930-1107.
Strain: C57BL/6J.
Tissue: Testis.
[4]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-438, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Mast cell.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1000; SER-1005; SER-1016 AND SER-1017, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1016 AND SER-1017, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC138373 mRNA. Translation: AAI38374.1.
BC145259 mRNA. Translation: AAI45260.1.
AK122429 mRNA. Translation: BAC65711.1.
AK015275 mRNA. Translation: BAB29777.1.
RefSeqNP_001074633.1. NM_001081164.1.
NP_001242962.1. NM_001256033.1.
UniGeneMm.34348.

3D structure databases

ProteinModelPortalB2RRE7.
SMRB2RRE7. Positions 2-152.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000035541.

Protein family/group databases

MEROPSC85.002.

PTM databases

PhosphoSiteB2RRE7.

Proteomic databases

PaxDbB2RRE7.
PRIDEB2RRE7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000173078; ENSMUSP00000133939; ENSMUSG00000036990.
ENSMUST00000173286; ENSMUSP00000134097; ENSMUSG00000036990.
GeneID73945.
KEGGmmu:73945.
UCSCuc009mir.1. mouse.

Organism-specific databases

CTD54726.
MGIMGI:1098801. Otud4.
RougeSearch...

Phylogenomic databases

eggNOGNOG81083.
GeneTreeENSGT00530000063508.
HOGENOMHOG000168383.
HOVERGENHBG082091.
InParanoidQ9CUN2.
KOK13718.
OMANPWFQEA.
OrthoDBEOG7H791H.
PhylomeDBB2RRE7.
TreeFamTF326812.

Gene expression databases

BgeeB2RRE7.
GenevestigatorB2RRE7.

Family and domain databases

InterProIPR003323. OTU.
[Graphical view]
PfamPF02338. OTU. 1 hit.
[Graphical view]
PROSITEPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSOTUD4. mouse.
NextBio339402.
PROB2RRE7.
SOURCESearch...

Entry information

Entry nameOTUD4_MOUSE
AccessionPrimary (citable) accession number: B2RRE7
Secondary accession number(s): B7ZNI5, Q80TL3, Q9CUN2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: July 1, 2008
Last modified: April 16, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot