ID   B2RQX9_MOUSE            Unreviewed;       791 AA.
AC   B2RQX9;
DT   01-JUL-2008, integrated into UniProtKB/TrEMBL.
DT   01-JUL-2008, sequence version 1.
DT   27-MAR-2024, entry version 110.
DE   RecName: Full=Transporter {ECO:0000256|RuleBase:RU003732};
GN   Name=Slc6a5 {ECO:0000313|EMBL:AAI38126.1,
GN   ECO:0000313|Ensembl:ENSMUSP00000058699.6, ECO:0000313|MGI:MGI:105090};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090 {ECO:0000313|EMBL:AAI38126.1};
RN   [1] {ECO:0000313|EMBL:AAI38126.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain {ECO:0000313|EMBL:AAI38126.1};
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RA   Gerhard D.S., Wagner L., Feingold E.A., Shenmen C.M., Grouse L.H.,
RA   Schuler G., Klein S.L., Old S., Rasooly R., Good P., Guyer M., Peck A.M.,
RA   Derge J.G., Lipman D., Collins F.S., Jang W., Sherry S., Feolo M.,
RA   Misquitta L., Lee E., Rotmistrovsky K., Greenhut S.F., Schaefer C.F.,
RA   Buetow K., Bonner T.I., Haussler D., Kent J., Kiekhaus M., Furey T.,
RA   Brent M., Prange C., Schreiber K., Shapiro N., Bhat N.K., Hopkins R.F.,
RA   Hsie F., Driscoll T., Soares M.B., Casavant T.L., Scheetz T.E.,
RA   Brown-stein M.J., Usdin T.B., Toshiyuki S., Carninci P., Piao Y.,
RA   Dudekula D.B., Ko M.S., Kawakami K., Suzuki Y., Sugano S., Gruber C.E.,
RA   Smith M.R., Simmons B., Moore T., Waterman R., Johnson S.L., Ruan Y.,
RA   Wei C.L., Mathavan S., Gunaratne P.H., Wu J., Garcia A.M., Hulyk S.W.,
RA   Fuh E., Yuan Y., Sneed A., Kowis C., Hodgson A., Muzny D.M., McPherson J.,
RA   Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S.,
RA   Sanchez A., Whiting M., Madari A., Young A.C., Wetherby K.D., Granite S.J.,
RA   Kwong P.N., Brinkley C.P., Pearson R.L., Bouffard G.G., Blakesly R.W.,
RA   Green E.D., Dickson M.C., Rodriguez A.C., Grimwood J., Schmutz J.,
RA   Myers R.M., Butterfield Y.S., Griffith M., Griffith O.L., Krzywinski M.I.,
RA   Liao N., Morin R., Morrin R., Palmquist D., Petrescu A.S., Skalska U.,
RA   Smailus D.E., Stott J.M., Schnerch A., Schein J.E., Jones S.J., Holt R.A.,
RA   Baross A., Marra M.A., Clifton S., Makowski K.A., Bosak S., Malek J.;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2] {ECO:0000313|Ensembl:ENSMUSP00000058699.6, ECO:0000313|Proteomes:UP000000589}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000058699.6,
RC   ECO:0000313|Proteomes:UP000000589};
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3] {ECO:0007829|PubMed:21183079}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [4] {ECO:0000313|Ensembl:ENSMUSP00000058699.6}
RP   IDENTIFICATION.
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000058699.6};
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF)
CC       (TC 2.A.22) family. {ECO:0000256|RuleBase:RU003732}.
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DR   EMBL; BC138125; AAI38126.1; -; mRNA.
DR   EMBL; BC138126; AAI38127.1; -; mRNA.
DR   RefSeq; NP_001139485.1; NM_001146013.1.
DR   RefSeq; NP_683733.2; NM_148931.3.
DR   RefSeq; XP_006540610.1; XM_006540547.1.
DR   ProteomicsDB; 332569; -.
DR   Antibodypedia; 25288; 89 antibodies from 22 providers.
DR   DNASU; 104245; -.
DR   Ensembl; ENSMUST00000056442.12; ENSMUSP00000058699.6; ENSMUSG00000039728.16.
DR   Ensembl; ENSMUST00000107605.3; ENSMUSP00000103230.3; ENSMUSG00000039728.16.
DR   Ensembl; ENSMUST00000207753.2; ENSMUSP00000146917.2; ENSMUSG00000039728.16.
DR   GeneID; 104245; -.
DR   KEGG; mmu:104245; -.
DR   UCSC; uc009hbv.3; mouse.
DR   AGR; MGI:105090; -.
DR   CTD; 9152; -.
DR   MGI; MGI:105090; Slc6a5.
DR   VEuPathDB; HostDB:ENSMUSG00000039728; -.
DR   GeneTree; ENSGT00940000154963; -.
DR   HOGENOM; CLU_006855_4_0_1; -.
DR   OMA; GAPKEMN; -.
DR   OrthoDB; 3084493at2759; -.
DR   TreeFam; TF343812; -.
DR   BioGRID-ORCS; 104245; 0 hits in 76 CRISPR screens.
DR   Proteomes; UP000000589; Chromosome 7.
DR   Bgee; ENSMUSG00000039728; Expressed in medial vestibular nucleus and 33 other cell types or tissues.
DR   GO; GO:0031045; C:dense core granule; IEA:Ensembl.
DR   GO; GO:0005768; C:endosome; IEA:Ensembl.
DR   GO; GO:0098690; C:glycinergic synapse; IEA:Ensembl.
DR   GO; GO:0042734; C:presynaptic membrane; IEA:Ensembl.
DR   GO; GO:0015375; F:glycine:sodium symporter activity; IEA:Ensembl.
DR   GO; GO:1903804; P:glycine import across plasma membrane; IEA:Ensembl.
DR   GO; GO:0060012; P:synaptic transmission, glycinergic; IEA:Ensembl.
DR   CDD; cd11499; SLC6sbd_GlyT2; 1.
DR   InterPro; IPR000175; Na/ntran_symport.
DR   InterPro; IPR037272; SNS_sf.
DR   PANTHER; PTHR11616:SF315; SODIUM- AND CHLORIDE-DEPENDENT GLYCINE TRANSPORTER 2; 1.
DR   PANTHER; PTHR11616; SODIUM/CHLORIDE DEPENDENT TRANSPORTER; 1.
DR   Pfam; PF00209; SNF; 1.
DR   PRINTS; PR00176; NANEUSMPORT.
DR   SUPFAM; SSF161070; SNF-like; 1.
DR   PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR   PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR   PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE   1: Evidence at protein level;
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Proteomics identification {ECO:0007829|MaxQB:B2RQX9,
KW   ECO:0007829|PeptideAtlas:B2RQX9};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000589};
KW   Symport {ECO:0000256|RuleBase:RU003732};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW   ECO:0000256|RuleBase:RU003732};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius};
KW   Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU003732}.
FT   TRANSMEM        223..245
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        266..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        386..403
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        415..448
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        468..485
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        497..522
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        556..577
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        598..623
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        629..652
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        673..692
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        712..733
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   REGION          1..56
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          85..112
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        85..99
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   791 AA;  87029 MW;  3400501C93EC4421 CRC64;
     MNKQPANILE AAVPGHRDSP RAPRTSPEQD LPAEAPTATV QPPRVPRSAS TGAQTFQSAD
     ARACEAQQSG VGFCNLSSPR AQATSAALRD LSEGHSAQAN PPSGPAGAGN ALHCKIPALR
     GPEEDANVSV GKGTLEHNNT PAVGWVNMSQ STVVLGTDGI ASVLPGSVAT TTIPEDEQGD
     ENKARGNWSS KLDFILSMVG YAVGLGNVWR FPYLAFQNGG GAFLIPYLMM LALAGLPIFF
     LEVSLGQFAS QGPVSVWKAI PALQGCGIAM LIISVLIAIY YNVIICYTLF YLFASFVSVL
     PWGSCNNPWN TPECKDKTKL LLDSCVIGDH PKIQIKNSTF CMTAYPNLTM VNFTSQTNKT
     FVSGSEEYFK YFVLKISAGI EYPGEIRWPL AFCLFLAWVI VYASLAKGIK SSGKVVYFTA
     TFPYVVLVIL LIRGVTLPGA GAGIWYFITP KWEKLTDATV WKDAATQIFF SLSAAWGGLI
     TLSSYNKFHN NCYRDTLIVT CTNSATSIFA GFVIFSVIGF MANERKVNIE NVADQGPGIA
     FVVYPEALTR LPLSPFWAII FFLMLLTLGL DTMFATIETI VTSISDEFPK YLRTHKPVFT
     LGCCICFFIM GFPMITQGGI YMFQLVDTYA ASYALVIIAI FELVGISYVY GLQRFCEDIE
     MMIGFKPNIF WKVCWAFVTP TILTFILCFS FYQWEPMTYG SYRYPNWSMV LGWLMLACSV
     IWIPIMFVIK MYLAPGRFIE RLKLVCSPQP DWGPFLAQHR GERYKNMIDP LGTSSLGLKL
     PVKDLELGTQ C
//