ID   UBP42_MOUSE             Reviewed;        1324 AA.
AC   B2RQC2;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   24-JAN-2024, entry version 103.
DE   RecName: Full=Ubiquitin carboxyl-terminal hydrolase 42;
DE            EC=3.4.19.12;
DE   AltName: Full=Deubiquitinating enzyme 42;
DE   AltName: Full=Ubiquitin thioesterase 42;
DE   AltName: Full=Ubiquitin-specific-processing protease 42;
GN   Name=Usp42;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX   PubMed=16904385; DOI=10.1016/j.modgep.2006.06.006;
RA   Kim Y.K., Kim Y.S., Yoo K.J., Lee H.J., Lee D.R., Yeo C.Y., Baek K.H.;
RT   "The expression of Usp42 during embryogenesis and spermatogenesis in
RT   mouse.";
RL   Gene Expr. Patterns 7:143-148(2007).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-858; SER-910; SER-1227;
RP   SER-1230 AND SER-1234, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP   ANALYSIS].
RC   TISSUE=Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Deubiquitinating enzyme which may play an important role
CC       during spermatogenesis. {ECO:0000269|PubMed:16904385}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC         and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC         residue protein attached to proteins as an intracellular targeting
CC         signal).; EC=3.4.19.12;
CC   -!- TISSUE SPECIFICITY: Highest expression seen in the testis. Also
CC       expressed in brain, lung and thymus. The expression level gradually
CC       increases from 2 weeks after birth and then decreases from the
CC       pachytene spermatocyte (PS) stage during spermatogenesis.
CC       {ECO:0000269|PubMed:16904385}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in the midbrain, forebrain, optic
CC       vesicles and eyes of embryos at 10.5 dpc and a more pronounced
CC       expression is seen at 12.5 dpc. {ECO:0000269|PubMed:16904385}.
CC   -!- SIMILARITY: Belongs to the peptidase C19 family. {ECO:0000305}.
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DR   EMBL; CH466529; EDL19037.1; -; Genomic_DNA.
DR   EMBL; BC137852; AAI37853.1; -; mRNA.
DR   CCDS; CCDS39372.1; -.
DR   RefSeq; NP_084025.2; NM_029749.2.
DR   RefSeq; XP_006504817.1; XM_006504754.3.
DR   AlphaFoldDB; B2RQC2; -.
DR   SMR; B2RQC2; -.
DR   BioGRID; 218324; 2.
DR   IntAct; B2RQC2; 1.
DR   MINT; B2RQC2; -.
DR   STRING; 10090.ENSMUSP00000053955; -.
DR   iPTMnet; B2RQC2; -.
DR   PhosphoSitePlus; B2RQC2; -.
DR   SwissPalm; B2RQC2; -.
DR   EPD; B2RQC2; -.
DR   MaxQB; B2RQC2; -.
DR   PaxDb; 10090-ENSMUSP00000053955; -.
DR   PeptideAtlas; B2RQC2; -.
DR   ProteomicsDB; 297704; -.
DR   Pumba; B2RQC2; -.
DR   Antibodypedia; 1719; 119 antibodies from 27 providers.
DR   DNASU; 76800; -.
DR   Ensembl; ENSMUST00000053287.6; ENSMUSP00000053955.6; ENSMUSG00000051306.8.
DR   GeneID; 76800; -.
DR   KEGG; mmu:76800; -.
DR   UCSC; uc009akp.1; mouse.
DR   AGR; MGI:1924050; -.
DR   CTD; 84132; -.
DR   MGI; MGI:1924050; Usp42.
DR   VEuPathDB; HostDB:ENSMUSG00000051306; -.
DR   eggNOG; KOG1865; Eukaryota.
DR   GeneTree; ENSGT00940000154596; -.
DR   HOGENOM; CLU_005541_1_0_1; -.
DR   InParanoid; B2RQC2; -.
DR   OMA; YGGRPYK; -.
DR   OrthoDB; 227085at2759; -.
DR   PhylomeDB; B2RQC2; -.
DR   TreeFam; TF315281; -.
DR   Reactome; R-MMU-5689880; Ub-specific processing proteases.
DR   BioGRID-ORCS; 76800; 1 hit in 75 CRISPR screens.
DR   ChiTaRS; Usp42; mouse.
DR   PRO; PR:B2RQC2; -.
DR   Proteomes; UP000000589; Chromosome 5.
DR   RNAct; B2RQC2; Protein.
DR   Bgee; ENSMUSG00000051306; Expressed in cumulus cell and 217 other cell types or tissues.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0004843; F:cysteine-type deubiquitinase activity; IDA:MGI.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0016579; P:protein deubiquitination; IDA:UniProtKB.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042981; P:regulation of apoptotic process; IBA:GO_Central.
DR   GO; GO:0007283; P:spermatogenesis; TAS:UniProtKB.
DR   CDD; cd02661; Peptidase_C19E; 1.
DR   Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR001394; Peptidase_C19_UCH.
DR   InterPro; IPR018200; USP_CS.
DR   InterPro; IPR028889; USP_dom.
DR   PANTHER; PTHR24006; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE; 1.
DR   PANTHER; PTHR24006:SF727; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 42; 1.
DR   Pfam; PF00443; UCH; 1.
DR   SUPFAM; SSF54001; Cysteine proteinases; 1.
DR   PROSITE; PS00972; USP_1; 1.
DR   PROSITE; PS00973; USP_2; 1.
DR   PROSITE; PS50235; USP_3; 1.
DR   Genevisible; B2RQC2; MM.
PE   1: Evidence at protein level;
KW   Differentiation; Hydrolase; Phosphoprotein; Protease; Reference proteome;
KW   Spermatogenesis; Thiol protease; Ubl conjugation pathway.
FT   CHAIN           1..1324
FT                   /note="Ubiquitin carboxyl-terminal hydrolase 42"
FT                   /id="PRO_0000404199"
FT   DOMAIN          110..411
FT                   /note="USP"
FT   REGION          1..38
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          64..86
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          417..517
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          549..587
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          624..676
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          717..780
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          816..847
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          880..1026
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1145..1262
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1303..1324
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..23
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        423..444
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        460..509
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        553..582
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        651..676
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        724..743
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        759..780
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        888..914
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        940..973
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1154..1200
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1201..1217
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1239..1253
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1303..1318
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        119
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10092,
FT                   ECO:0000255|PROSITE-ProRule:PRU10093"
FT   ACT_SITE        370
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10092,
FT                   ECO:0000255|PROSITE-ProRule:PRU10093"
FT   MOD_RES         74
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H9J4"
FT   MOD_RES         482
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H9J4"
FT   MOD_RES         749
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H9J4"
FT   MOD_RES         858
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         910
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         1189
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H9J4"
FT   MOD_RES         1227
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         1230
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         1234
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         1255
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H9J4"
SQ   SEQUENCE   1324 AA;  146223 MW;  5214E845D98C3288 CRC64;
     MTIVDKTEPS DPSTCQNQPG SCEAVSPEDM DTGSASWGAV SSISDVSSHT LPLGPVPGAV
     VYSNSSVPEK SKPSPPKDQV LGDGIAPPQK VLFPSEKICL KWQQSHRVGA GLQNLGNTCF
     ANAALQCLTY TPPLANYMLS HEHSKTCHAE GFCMMCTMQT HITQALSNPG DVIKPMFVIN
     EMRRIARHFR FGNQEDAHEF LQYTVDAMQK ACLNGSNKLD RHTQATTLVC QIFGGYLRSR
     VKCLNCKGVS DTFDPYLDIT LEIKAAQSVT KALEQFVKPE QLDGENSYKC SKCKKMVPAS
     KRFTIHRSSN VLTISLKRFA NFTGGKIAKD VKYPEYLDIR PYMSQPNGEP IIYVLYAVLV
     HTGFNCHAGH YFCYIKASNG LWYQMNDSIV STSDIRAVLN QQAYVLFYIR SHDVKNGGES
     AHPAHSPGQS SPRPGVSQRV VNNKQVAPGF IGPQLPSHVM KNTPHLNGTT PVKDTPSSSV
     SSPNGNTSVN RASPATASTS VQNWSVTRPS VIPDHPKKQK ITISIHNKLP ARQGQAPLNN
     SLHGPCLEAP SKAAPSSTIT NPSAIQSTSN VPTTSTSPSE ACPKPMVNGK AKVGASVLVP
     YGAESSEESD EESKGLAKEN GVDMMAGTHS DRPEAAADDG AEASSHELQE PVLLNGANSA
     DSDSQENSLA FDSASCQVQP ELHTENLFSK LNGLPGKVTP APLQSVPEDR ILETFKLTNQ
     AKGPAGEESW TTTGGSSPKD PVSQLEPISD EPSPLEIPEA VTNGSTQTPS TTSPLEPTIS
     CTKEDSSVVV SAEPVEGLPS VPALCNSTGT ILGDTPVPEL CDPGDLTANP SQPTEAVKGD
     TAEKAQDSAM AEVVERLSPA PSVLTGDGCE QKLLLYLSAE GSEETEDSSR SSAVSADTMP
     PKPDRTTTSS CEGAAEQAAG DRGDGGHVGP KAQEPSPAKE KMSSLRKVDR GHYRSRRERS
     SSGEHVRDSR PRPEDHHHKK RHCYSRERPK QDRHPTNSYC NGGQHLGHGD RASPERRSLS
     RYSHHHSRIR SGLEQDWSRY HHLENEHAWV RERFYQDKLR WDKCRYYHDR YTPLYTARDA
     REWRPLHGRE HDRLVQSGRP YKDSYWGRKG WELQSRGKER PHFNSPREAP SLAVPLERHL
     QEKAALSVQD SSHSLPERFH EHKSVKSRKR RYETLENNDG RLEKKVHKSL EKDTLEEPRV
     KKHKKSKKKK KSKDKHRDRE SRHQQESDFS GAYSDADLHR HRKKKKKKKR HSRKSEDFIK
     DVEMRLPKLS SYEAGGHFRR TEGSFLLADG LPVEDSGPFR EKTKHLRMES RPDRCRLSEY
     GQGD
//