B2RQC2 (UBP42_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 42
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 42
Ubiquitin thioesterase 42
Ubiquitin-specific-processing protease 42

Gene names

Name:

Usp42

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1324 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Deubiquitinating enzyme which may play an important role during spermatogenesis. Ref.3
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Tissue specificity	Highest expression seen in the testis. Also expressed in brain, lung and thymus. The expression level gradually increases from 2 weeks after birth and then decreases from the pachytene spermatocyte (PS) stage during spermatogenesis. Ref.3
Developmental stage	Expressed in the midbrain, forebrain, optic vesicles and eyes of embryos at E10.5 and a more pronounced expression is seen at E12.5. Ref.3
Sequence similarities	Belongs to the peptidase C19 family.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW protein deubiquitination Inferred from direct assay Ref.3. Source: UniProtKB spermatogenesis Traceable author statement Ref.3. Source: UniProtKB ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Inferred from direct assay Ref.3. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1324	1324	Ubiquitin carboxyl-terminal hydrolase 42		PRO_0000404199
Regions
	Compositional bias	1184 – 1254	71	Lys-rich
Sites
	Active site	119	1	Nucleophile By similarity
	Active site	370	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	858	1	Phosphoserine By similarity
	Modified residue	1234	1	Phosphoserine By similarity
	Modified residue	1255	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2RQC2 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 5214E845D98C3288	FASTA	1,324	146,223
	10 20 30 40 50 60 MTIVDKTEPS DPSTCQNQPG SCEAVSPEDM DTGSASWGAV SSISDVSSHT LPLGPVPGAV 70 80 90 100 110 120 VYSNSSVPEK SKPSPPKDQV LGDGIAPPQK VLFPSEKICL KWQQSHRVGA GLQNLGNTCF 130 140 150 160 170 180 ANAALQCLTY TPPLANYMLS HEHSKTCHAE GFCMMCTMQT HITQALSNPG DVIKPMFVIN 190 200 210 220 230 240 EMRRIARHFR FGNQEDAHEF LQYTVDAMQK ACLNGSNKLD RHTQATTLVC QIFGGYLRSR 250 260 270 280 290 300 VKCLNCKGVS DTFDPYLDIT LEIKAAQSVT KALEQFVKPE QLDGENSYKC SKCKKMVPAS 310 320 330 340 350 360 KRFTIHRSSN VLTISLKRFA NFTGGKIAKD VKYPEYLDIR PYMSQPNGEP IIYVLYAVLV 370 380 390 400 410 420 HTGFNCHAGH YFCYIKASNG LWYQMNDSIV STSDIRAVLN QQAYVLFYIR SHDVKNGGES 430 440 450 460 470 480 AHPAHSPGQS SPRPGVSQRV VNNKQVAPGF IGPQLPSHVM KNTPHLNGTT PVKDTPSSSV 490 500 510 520 530 540 SSPNGNTSVN RASPATASTS VQNWSVTRPS VIPDHPKKQK ITISIHNKLP ARQGQAPLNN 550 560 570 580 590 600 SLHGPCLEAP SKAAPSSTIT NPSAIQSTSN VPTTSTSPSE ACPKPMVNGK AKVGASVLVP 610 620 630 640 650 660 YGAESSEESD EESKGLAKEN GVDMMAGTHS DRPEAAADDG AEASSHELQE PVLLNGANSA 670 680 690 700 710 720 DSDSQENSLA FDSASCQVQP ELHTENLFSK LNGLPGKVTP APLQSVPEDR ILETFKLTNQ 730 740 750 760 770 780 AKGPAGEESW TTTGGSSPKD PVSQLEPISD EPSPLEIPEA VTNGSTQTPS TTSPLEPTIS 790 800 810 820 830 840 CTKEDSSVVV SAEPVEGLPS VPALCNSTGT ILGDTPVPEL CDPGDLTANP SQPTEAVKGD 850 860 870 880 890 900 TAEKAQDSAM AEVVERLSPA PSVLTGDGCE QKLLLYLSAE GSEETEDSSR SSAVSADTMP 910 920 930 940 950 960 PKPDRTTTSS CEGAAEQAAG DRGDGGHVGP KAQEPSPAKE KMSSLRKVDR GHYRSRRERS 970 980 990 1000 1010 1020 SSGEHVRDSR PRPEDHHHKK RHCYSRERPK QDRHPTNSYC NGGQHLGHGD RASPERRSLS 1030 1040 1050 1060 1070 1080 RYSHHHSRIR SGLEQDWSRY HHLENEHAWV RERFYQDKLR WDKCRYYHDR YTPLYTARDA 1090 1100 1110 1120 1130 1140 REWRPLHGRE HDRLVQSGRP YKDSYWGRKG WELQSRGKER PHFNSPREAP SLAVPLERHL 1150 1160 1170 1180 1190 1200 QEKAALSVQD SSHSLPERFH EHKSVKSRKR RYETLENNDG RLEKKVHKSL EKDTLEEPRV 1210 1220 1230 1240 1250 1260 KKHKKSKKKK KSKDKHRDRE SRHQQESDFS GAYSDADLHR HRKKKKKKKR HSRKSEDFIK 1270 1280 1290 1300 1310 1320 DVEMRLPKLS SYEAGGHFRR TEGSFLLADG LPVEDSGPFR EKTKHLRMES RPDRCRLSEY GQGD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The expression of Usp42 during embryogenesis and spermatogenesis in mouse." Kim Y.K., Kim Y.S., Yoo K.J., Lee H.J., Lee D.R., Yeo C.Y., Baek K.H. Gene Expr. Patterns 7:143-148(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH466529 Genomic DNA. Translation: EDL19037.1. BC137852 mRNA. Translation: AAI37853.1.
IPI	IPI00352204.
RefSeq	NP_084025.2. NM_029749.2.
UniGene	Mm.259620.
3D structure databases
ProteinModelPortal	B2RQC2.
SMR	B2RQC2. Positions 110-408.
ModBase	Search...
PTM databases
PhosphoSite	B2RQC2.
Proteomic databases
PaxDb	B2RQC2.
PRIDE	B2RQC2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000053287; ENSMUSP00000053955; ENSMUSG00000051306.
GeneID	76800.
KEGG	mmu:76800.
UCSC	uc009akp.1. mouse.
Organism-specific databases
CTD	84132.
MGI	MGI:1924050. Usp42.
Phylogenomic databases
eggNOG	COG5533.
GeneTree	ENSGT00670000097591.
HOGENOM	HOG000236355.
HOVERGEN	HBG080724.
InParanoid	B2RQC2.
KO	K11855.
OMA	ALHSENP.
OrthoDB	EOG4RV2RB.
Gene expression databases
Bgee	B2RQC2.
Genevestigator	B2RQC2.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	345843.
SOURCE	Search...

Entry information

Entry name

UBP42_MOUSE

Accession

Primary (citable) accession number: B2RQC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 8, 2011
Last sequence update:	July 1, 2008
Last modified:	May 1, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program