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Protein

Ubiquitin carboxyl-terminal hydrolase 42

Gene

Usp42

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme which may play an important role during spermatogenesis.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei119 – 1191NucleophilePROSITE-ProRule annotation
Active sitei370 – 3701Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  • cysteine-type endopeptidase activity Source: GO_Central
  • ubiquitin-specific protease activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Differentiation, Spermatogenesis, Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.048.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 42 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 42
Ubiquitin thioesterase 42
Ubiquitin-specific-processing protease 42
Gene namesi
Name:Usp42
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1924050. Usp42.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13241324Ubiquitin carboxyl-terminal hydrolase 42PRO_0000404199Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei858 – 8581PhosphoserineBy similarity
Modified residuei1234 – 12341PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiB2RQC2.
PaxDbiB2RQC2.
PRIDEiB2RQC2.

PTM databases

PhosphoSiteiB2RQC2.

Expressioni

Tissue specificityi

Highest expression seen in the testis. Also expressed in brain, lung and thymus. The expression level gradually increases from 2 weeks after birth and then decreases from the pachytene spermatocyte (PS) stage during spermatogenesis.1 Publication

Developmental stagei

Expressed in the midbrain, forebrain, optic vesicles and eyes of embryos at E10.5 and a more pronounced expression is seen at E12.5.1 Publication

Gene expression databases

BgeeiB2RQC2.
GenevestigatoriB2RQC2.

Interactioni

Protein-protein interaction databases

BioGridi218324. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliB2RQC2.
SMRiB2RQC2. Positions 110-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini110 – 411302USPAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1184 – 125471Lys-richAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00790000123004.
HOGENOMiHOG000236355.
HOVERGENiHBG080724.
InParanoidiB2RQC2.
KOiK11855.
OMAiGKLMPAP.
OrthoDBiEOG72VH5K.
PhylomeDBiB2RQC2.
TreeFamiTF315281.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2RQC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIVDKTEPS DPSTCQNQPG SCEAVSPEDM DTGSASWGAV SSISDVSSHT
60 70 80 90 100
LPLGPVPGAV VYSNSSVPEK SKPSPPKDQV LGDGIAPPQK VLFPSEKICL
110 120 130 140 150
KWQQSHRVGA GLQNLGNTCF ANAALQCLTY TPPLANYMLS HEHSKTCHAE
160 170 180 190 200
GFCMMCTMQT HITQALSNPG DVIKPMFVIN EMRRIARHFR FGNQEDAHEF
210 220 230 240 250
LQYTVDAMQK ACLNGSNKLD RHTQATTLVC QIFGGYLRSR VKCLNCKGVS
260 270 280 290 300
DTFDPYLDIT LEIKAAQSVT KALEQFVKPE QLDGENSYKC SKCKKMVPAS
310 320 330 340 350
KRFTIHRSSN VLTISLKRFA NFTGGKIAKD VKYPEYLDIR PYMSQPNGEP
360 370 380 390 400
IIYVLYAVLV HTGFNCHAGH YFCYIKASNG LWYQMNDSIV STSDIRAVLN
410 420 430 440 450
QQAYVLFYIR SHDVKNGGES AHPAHSPGQS SPRPGVSQRV VNNKQVAPGF
460 470 480 490 500
IGPQLPSHVM KNTPHLNGTT PVKDTPSSSV SSPNGNTSVN RASPATASTS
510 520 530 540 550
VQNWSVTRPS VIPDHPKKQK ITISIHNKLP ARQGQAPLNN SLHGPCLEAP
560 570 580 590 600
SKAAPSSTIT NPSAIQSTSN VPTTSTSPSE ACPKPMVNGK AKVGASVLVP
610 620 630 640 650
YGAESSEESD EESKGLAKEN GVDMMAGTHS DRPEAAADDG AEASSHELQE
660 670 680 690 700
PVLLNGANSA DSDSQENSLA FDSASCQVQP ELHTENLFSK LNGLPGKVTP
710 720 730 740 750
APLQSVPEDR ILETFKLTNQ AKGPAGEESW TTTGGSSPKD PVSQLEPISD
760 770 780 790 800
EPSPLEIPEA VTNGSTQTPS TTSPLEPTIS CTKEDSSVVV SAEPVEGLPS
810 820 830 840 850
VPALCNSTGT ILGDTPVPEL CDPGDLTANP SQPTEAVKGD TAEKAQDSAM
860 870 880 890 900
AEVVERLSPA PSVLTGDGCE QKLLLYLSAE GSEETEDSSR SSAVSADTMP
910 920 930 940 950
PKPDRTTTSS CEGAAEQAAG DRGDGGHVGP KAQEPSPAKE KMSSLRKVDR
960 970 980 990 1000
GHYRSRRERS SSGEHVRDSR PRPEDHHHKK RHCYSRERPK QDRHPTNSYC
1010 1020 1030 1040 1050
NGGQHLGHGD RASPERRSLS RYSHHHSRIR SGLEQDWSRY HHLENEHAWV
1060 1070 1080 1090 1100
RERFYQDKLR WDKCRYYHDR YTPLYTARDA REWRPLHGRE HDRLVQSGRP
1110 1120 1130 1140 1150
YKDSYWGRKG WELQSRGKER PHFNSPREAP SLAVPLERHL QEKAALSVQD
1160 1170 1180 1190 1200
SSHSLPERFH EHKSVKSRKR RYETLENNDG RLEKKVHKSL EKDTLEEPRV
1210 1220 1230 1240 1250
KKHKKSKKKK KSKDKHRDRE SRHQQESDFS GAYSDADLHR HRKKKKKKKR
1260 1270 1280 1290 1300
HSRKSEDFIK DVEMRLPKLS SYEAGGHFRR TEGSFLLADG LPVEDSGPFR
1310 1320
EKTKHLRMES RPDRCRLSEY GQGD
Length:1,324
Mass (Da):146,223
Last modified:July 1, 2008 - v1
Checksum:i5214E845D98C3288
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH466529 Genomic DNA. Translation: EDL19037.1.
BC137852 mRNA. Translation: AAI37853.1.
CCDSiCCDS39372.1.
RefSeqiNP_084025.2. NM_029749.2.
XP_006504817.1. XM_006504754.2.
UniGeneiMm.259620.

Genome annotation databases

EnsembliENSMUST00000053287; ENSMUSP00000053955; ENSMUSG00000051306.
GeneIDi76800.
KEGGimmu:76800.
UCSCiuc009akp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH466529 Genomic DNA. Translation: EDL19037.1.
BC137852 mRNA. Translation: AAI37853.1.
CCDSiCCDS39372.1.
RefSeqiNP_084025.2. NM_029749.2.
XP_006504817.1. XM_006504754.2.
UniGeneiMm.259620.

3D structure databases

ProteinModelPortaliB2RQC2.
SMRiB2RQC2. Positions 110-408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218324. 1 interaction.

Protein family/group databases

MEROPSiC19.048.

PTM databases

PhosphoSiteiB2RQC2.

Proteomic databases

MaxQBiB2RQC2.
PaxDbiB2RQC2.
PRIDEiB2RQC2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053287; ENSMUSP00000053955; ENSMUSG00000051306.
GeneIDi76800.
KEGGimmu:76800.
UCSCiuc009akp.1. mouse.

Organism-specific databases

CTDi84132.
MGIiMGI:1924050. Usp42.

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00790000123004.
HOGENOMiHOG000236355.
HOVERGENiHBG080724.
InParanoidiB2RQC2.
KOiK11855.
OMAiGKLMPAP.
OrthoDBiEOG72VH5K.
PhylomeDBiB2RQC2.
TreeFamiTF315281.

Miscellaneous databases

NextBioi345843.
PROiB2RQC2.
SOURCEiSearch...

Gene expression databases

BgeeiB2RQC2.
GenevestigatoriB2RQC2.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "The expression of Usp42 during embryogenesis and spermatogenesis in mouse."
    Kim Y.K., Kim Y.S., Yoo K.J., Lee H.J., Lee D.R., Yeo C.Y., Baek K.H.
    Gene Expr. Patterns 7:143-148(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiUBP42_MOUSE
AccessioniPrimary (citable) accession number: B2RQC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: July 1, 2008
Last modified: May 27, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.