B2RLM4 (KDSB_PORG3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-deoxy-manno-octulosonate cytidylyltransferase
EC=2.7.7.38

Alternative name(s):
CMP-2-keto-3-deoxyoctulosonic acid synthase
Short name=CKS
Short name=CMP-KDO synthase

Gene names

Name:	kdsB
Ordered Locus Names:	PGN_1750

Organism

Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / JCM 12257) [Complete proteome] [HAMAP]

Taxonomic identifier

431947 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Porphyromonas

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria By similarity. HAMAP MF_00057
Catalytic activity	CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate. HAMAP MF_00057
Pathway	Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP: step 1/1. HAMAP MF_00057 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00057
Subcellular location	Cytoplasm Potential HAMAP MF_00057.
Sequence similarities	Belongs to the KdsB family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-manno-octulosonate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	3-deoxy-manno-octulosonate cytidylyltransferase HAMAP MF_00057		PRO_0000370119

Sequences

	Sequence		Length	Mass (Da)
	B2RLM4 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 65390E34F9C13E27	FASTA	254	28,660
	10 20 30 40 50 60 MNTEVIAIIP ARFASSRFPG KPLADMLGKS MIQRVHERIA GVVPRAVVAT DDERIRQAVE 70 80 90 100 110 120 DFGGEVVMTS PECSSGTERC REAFDKVGRG EKIVLNLQGD EPFIQKEQID LLISAFDKPE 130 140 150 160 170 180 TDIATLAEVF SSDASFERLN NPNSPKIVLD HGGYALYFSR SVIPYLRGVQ PDSWCRRHTY 190 200 210 220 230 240 YKHIGIYAFR PTVLRKITSL PQSTAEQAES LEQLRWLEYG YRIRVLQTQQ STIGIDTPED 250 MDKAIAYLRS QGME

References

[1]

"Determination of the genome sequence of Porphyromonas gingivalis strain ATCC 33277 and genomic comparison with strain W83 revealed extensive genome rearrangements in P. gingivalis." Naito M., Hirakawa H., Yamashita A., Ohara N., Shoji M., Yukitake H., Nakayama K., Toh H., Yoshimura F., Kuhara S., Hattori M., Hayashi T., Nakayama K. DNA Res. 15:215-225(2008) [PubMed: 18524787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33277 / DSM 20709 / JCM 12257.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009380 Genomic DNA. Translation: BAG34269.1.
RefSeq	YP_001929866.1. NC_010729.1.
3D structure databases
ProteinModelPortal	B2RLM4.
ModBase	Search...
Protein-protein interaction databases
STRING	B2RLM4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6329523.
GenomeReviews	Gene locus PGN_1750 in contig AP009380_GR.
KEGG	pgn:PGN_1750.
PATRIC	22976473. VBIPorGin26334_1726.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG637773.
OMA	NTRQDAL.
ProtClustDB	PRK05450.
Family and domain databases
HAMAP	MF_00057. CMP-KDO_synth. [Tree]
InterPro	IPR003329. Cytidylyl_trans. IPR004528. KdsB. [Graphical view]
KO	K00979.
PANTHER	PTHR21485:SF4. PTHR21485:SF4. 1 hit.
Pfam	PF02348. CTP_transf_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00466. KdsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSB_PORG3

Accession

Primary (citable) accession number: B2RLM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program