B2RJI6 (ISPE_PORG3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	PGN_1012

Organism

Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / JCM 12257) [Complete proteome] [HAMAP]

Taxonomic identifier

431947 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Porphyromonas

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000092103
Regions
	Nucleotide binding	92 – 102	11	ATP Potential
Sites
	Active site	8	1	By similarity
	Active site	134	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2RJI6 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 230DBD1DC5BAD71E	FASTA	274	30,232
	10 20 30 40 50 60 MIVFPNAKIN LGLQVVAKRA DGYHNIETVF YPIPLTDALE IEVREDTCDR LSVHGIPIDA 70 80 90 100 110 120 ATEDNLVMKA ILALRRKFDF PPLTIELIKH IPSGAGLGGG SSNASFMLKL VRDYFSLPID 130 140 150 160 170 180 DKELAAIALT IGADCPFFVG NRPVLATDLG QVFTPLPNFS LSGLHIAIVK PPIHINTAAA 190 200 210 220 230 240 YKGLKHVGKR ETMPDEIVYM PVDEWRGKLV NDFEESLFPE HPRLAELKEM LYRSGALYAA 250 260 270 MSGSGSALFG LFREKPQLDK AAITDCFRWQ SIIP

References

[1]

"Determination of the genome sequence of Porphyromonas gingivalis strain ATCC 33277 and genomic comparison with strain W83 revealed extensive genome rearrangements in P. gingivalis." Naito M., Hirakawa H., Yamashita A., Ohara N., Shoji M., Yukitake H., Nakayama K., Toh H., Yoshimura F., Kuhara S., Hattori M., Hayashi T., Nakayama K. DNA Res. 15:215-225(2008) [PubMed: 18524787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33277 / DSM 20709 / JCM 12257.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009380 Genomic DNA. Translation: BAG33531.1.
RefSeq	YP_001929128.1. NC_010729.1.
3D structure databases
ProteinModelPortal	B2RJI6.
ModBase	Search...
Protein-protein interaction databases
STRING	B2RJI6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6328872.
GenomeReviews	Gene locus PGN_1012 in contig AP009380_GR.
KEGG	pgn:PGN_1012.
PATRIC	22974978. VBIPorGin26334_1002.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	VSTAEVY.
ProtClustDB	PRK14609.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_PORG3

Accession

Primary (citable) accession number: B2RJI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program