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B2RIZ3 (SYL_PORG3) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PGN_0819
OrganismPorphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / JCM 12257) [Complete proteome] [HAMAP]
Taxonomic identifier431947 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesPorphyromonadaceaePorphyromonas

Protein attributes

Sequence length925 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 925925Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091343

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif700 – 7045"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7031ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B2RIZ3 [UniParc].

Last modified July 1, 2008. Version 1.
Checksum: D1E774CE15ABA339

FASTA925106,051
        10         20         30         40         50         60 
MEYNFQEIEK RVQGQWRKDK VYRVAEDTSK KPFYVLDMFP YPSGAGLHVG HPLGYIASDI 

        70         80         90        100        110        120 
FSRYKRLRGF NVLHPMGYDA FGLPAEQYAI QTGQHPEKTT EENTARYREQ LDKIGFSYDW 

       130        140        150        160        170        180 
EREIRTCDPN YYKWTQWAFL KMFDSYYCNQ AQQARPISEL IEVFARQGNE GLNVACGEGV 

       190        200        210        220        230        240 
RFTAEEWTAM SEKEQQEILM NYRLAYLGDT MVNWCPALGT VLANDEVKDG VSERGGHPVE 

       250        260        270        280        290        300 
QKKMRQWCLR VSAYAERLLH DLETLDWTES LKETQRNWIG RSEGAEMEFR LAGKDCTFTI 

       310        320        330        340        350        360 
FTTRADTIFG VTFMVLAPES ELVEEVTTEE QRAAVETYLT ETKRRTERER ISDKRVSGVF 

       370        380        390        400        410        420 
SGSYAINPLT GKEIPIWISD YVLAGYGTGA IMAVPAHDTR DFAFARHFDL PIVQVVVPEG 

       430        440        450        460        470        480 
ETATDPATWE DAKDSKSGIM VNSDFLNGLS VEDAIAQTKE YIREKHLGCV KVNYRLRDAI 

       490        500        510        520        530        540 
FSRQRYWGEP FPIYYKEGMP HALDEDRLPL RLPEVDKFLP TESGEPPLGR ATGWHTAEGY 

       550        560        570        580        590        600 
PYELSTMPGF AGSSAYYLRY MDPQNDTALV SRSANEYWRH VDLYIGGTEH ATGHLIYSRF 

       610        620        630        640        650        660 
WNKFLFDLGI VCEAEPFRKL VNQGMIQGRS NFVYRIKNTN TFVSYGLREQ YEVTPLHVDV 

       670        680        690        700        710        720 
NIVSNDQLDI DRFRAWRPEY ASAEFILEDG KYICGWAIEK MSKSMFNVVN PDDIIARYGA 

       730        740        750        760        770        780 
DTLRLYEMFL GPLEQSKPWD TNGIDGVHRF LKKFWALYYN ADGIRVTDTA PTKEELKSLH 

       790        800        810        820        830        840 
KLIKKVGQDI ESFSFNTSIP AFMICVNELT AAKTTSRAIL CPLLTVLSPF APHITEWLWQ 

       850        860        870        880        890        900 
ELGVEGSIVT ATWPEYNEEY LVESCVRYPV SFNGKVRFNI ELPADMSKKD VEQAALTAPE 

       910        920 
AARWLEGKSP KKVIVVPGRI VNVVV 

« Hide

References

[1]"Determination of the genome sequence of Porphyromonas gingivalis strain ATCC 33277 and genomic comparison with strain W83 revealed extensive genome rearrangements in P. gingivalis."
Naito M., Hirakawa H., Yamashita A., Ohara N., Shoji M., Yukitake H., Nakayama K., Toh H., Yoshimura F., Kuhara S., Hattori M., Hayashi T., Nakayama K.
DNA Res. 15:215-225(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33277 / DSM 20709 / JCM 12257.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009380 Genomic DNA. Translation: BAG33338.1.
RefSeqYP_001928935.1. NC_010729.1.

3D structure databases

ProteinModelPortalB2RIZ3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING431947.PGN_0819.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAG33338; BAG33338; PGN_0819.
GeneID6329348.
KEGGpgn:PGN_0819.
PATRIC22974582. VBIPorGin26334_0808.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPGIN431947:GC9J-846-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PORG3
AccessionPrimary (citable) accession number: B2RIZ3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 1, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries