B2RIE9 (MURD_PORG3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	PGN_0625

Organism

Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / JCM 12257) [Complete proteome] [HAMAP]

Taxonomic identifier

431947 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Porphyromonas

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_1000130872
Regions
	Nucleotide binding	113 – 119	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B2RIE9 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 2F1270C9D660033B	FASTA	450	50,565
	10 20 30 40 50 60 MDRLKYDIVV LGAGESGVGA ALLAQAKGLH VFVSDYGKIA PKYKEELNRY AIPYEEGRHT 70 80 90 100 110 120 EAIILEAKEI IKSPGIPDTA PVIRQAVAKE IGIVSEIEFA GRYTDAFMVC ITGSNGKTTT 130 140 150 160 170 180 TMWLYHTLCK AGLDVGLAGN VGFSLARQVA YDPHPYYVIE LSSFQLDNMY DFRANVAILL 190 200 210 220 230 240 NITPDHLDRY DHRFELYAEA KMRITRNQQP EDCFIYWEDD PFISRWVAEH PPVARLLPFA 250 260 270 280 290 300 MEARTDNTTA WINEKNELVV MNLNSPFVMD EELLALSGMH NRHNAMATAI AAKAMDIKNE 310 320 330 340 350 360 AIREALQDFK NVPHRLEKIA RVKGVDYIND SKATNVNSTW YALESMKTRV ILILGGTDKG 370 380 390 400 410 420 NDYTDIENLV LSKVDGLIFL GIDNEKLHKF FDGKISRIAD ACSMHEAVSL AYKMASKGDT 430 440 450 VLLSPACASF DLFQNYEDRG DQFRKEVLNL

References

[1]

"Determination of the genome sequence of Porphyromonas gingivalis strain ATCC 33277 and genomic comparison with strain W83 revealed extensive genome rearrangements in P. gingivalis." Naito M., Hirakawa H., Yamashita A., Ohara N., Shoji M., Yukitake H., Nakayama K., Toh H., Yoshimura F., Kuhara S., Hattori M., Hayashi T., Nakayama K. DNA Res. 15:215-225(2008) [PubMed: 18524787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33277 / DSM 20709 / JCM 12257.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009380 Genomic DNA. Translation: BAG33144.1.
RefSeq	YP_001928741.1. NC_010729.1.
3D structure databases
ProteinModelPortal	B2RIE9.
ModBase	Search...
Protein-protein interaction databases
STRING	B2RIE9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6329657.
GenomeReviews	Gene locus PGN_0625 in contig AP009380_GR.
KEGG	pgn:PGN_0625.
PATRIC	22974196. VBIPorGin26334_0619.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG750024.
OMA	ACASWDM.
ProtClustDB	CLSK2760238.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_PORG3

Accession

Primary (citable) accession number: B2RIE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program