B2RHF5 (SYM_PORG3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	PGN_0281

Organism

Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / JCM 12257) [Complete proteome] [HAMAP]

Taxonomic identifier

431947 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Porphyromonas › Porphyromonas gingivalis

Protein attributes

Sequence length	680 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 680	680	Methionine--tRNA ligase HAMAP-Rule MF_00098		PRO_1000093722
Regions
	Domain	579 – 680	102	tRNA-binding
	Motif	15 – 25	11	"HIGH" region HAMAP-Rule MF_00098
	Motif	332 – 336	5	"KMSKS" region HAMAP-Rule MF_00098
Sites
	Metal binding	147	1	Zinc By similarity
	Metal binding	150	1	Zinc By similarity
	Metal binding	160	1	Zinc By similarity
	Metal binding	163	1	Zinc By similarity
	Binding site	335	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2RHF5 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: F24B299D01B1BA46	FASTA	680	76,669
	10 20 30 40 50 60 MDRQFKRTLI TTALPYANGP VHIGHLAGVY VPADIYARYL RLRGRDCLLI GGSDEHGVPI 70 80 90 100 110 120 ALKAKAEGCT PQEVVDRYHE LIKRSFEGLG ISFDIYSRTT SDIHRRTASE FFKTLYEKGE 130 140 150 160 170 180 FVEQTSEQYY DEEAKQFLAD RYIIGTCPHC SNDRAYGDQC EACGTSLNAT DLIDPRSTIS 190 200 210 220 230 240 GSAPVLRETK HWYLPLDKHE PFLKEWILDG HKEWKSNVYG QCKSWLDMGL QPRAVSRDLD 250 260 270 280 290 300 WGIPVPVEGA EGKVLYVWFD APIGYISNTK ELCPDSWETW WKSEDTRLVH FIGKDNIVFH 310 320 330 340 350 360 CIVFPAMLRA EGSFILPDNV PANEFLNLEG DKISTSRNWA VWLHEYLEEF PGKQDVLRYV 370 380 390 400 410 420 LTANAPETKD NDFTWRDFQA RNNNELVAIF GNFVNRALVL THKYFDGAVP PKGELTDYDS 430 440 450 460 470 480 RTIEEFAAVK QALEHQLDTF HFREALKEAM NLARIGNKYL ADTEPWKLAK TDMNRVATIL 490 500 510 520 530 540 NLSLQITANL AIAFEPFLPF SSAKLMSMLS TDCPFGWDRL GSTDLLPEGH VLGNPELLFE 550 560 570 580 590 600 KLEDSVIDAQ IQKLQDTKLA NEKAAHQAAP IAEDIAFEDF LKLDIRVGTV LECEKVPKAD 610 620 630 640 650 660 KLLRFRIDDG LGGRTIVSGI AKHYAPEELV GKQVCFIANL PPRKLKGIES EGMILSAEDA 670 680 DGSLRVIMPA AEVAPGSQVK

References

[1]

"Determination of the genome sequence of Porphyromonas gingivalis strain ATCC 33277 and genomic comparison with strain W83 revealed extensive genome rearrangements in P. gingivalis." Naito M., Hirakawa H., Yamashita A., Ohara N., Shoji M., Yukitake H., Nakayama K., Toh H., Yoshimura F., Kuhara S., Hattori M., Hayashi T., Nakayama K. DNA Res. 15:215-225(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33277 / DSM 20709 / JCM 12257.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009380 Genomic DNA. Translation: BAG32800.1.
RefSeq	YP_001928397.1. NC_010729.1.
3D structure databases
ProteinModelPortal	B2RHF5.
ModBase	Search...
Protein-protein interaction databases
STRING	431947.PGN_0281.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAG32800; BAG32800; PGN_0281.
GeneID	6330024.
KEGG	pgn:PGN_0281.
PATRIC	22973496. VBIPorGin26334_0279.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0143.
HOGENOM	HOG000200402.
KO	K01874.
OMA	HEIAPFK.
ProtClustDB	PRK00133.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00098. Met_tRNA_synth_type1.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR023458. Met-tRNA_ligase_1. IPR014758. Met-tRNA_synth. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_PORG3

Accession

Primary (citable) accession number: B2RHF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 1, 2008
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program