B2RGM5 (DNAA_PORG3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	PGN_0001

Organism

Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / JCM 12257) [Complete proteome] [HAMAP]

Taxonomic identifier

431947 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Porphyromonas

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity HAMAP MF_00377.
Sequence similarities	Belongs to the DnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from electronic annotation. Source: InterPro regulation of DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Chromosomal replication initiator protein DnaA HAMAP MF_00377		PRO_1000122001
Regions
	Nucleotide binding	178 – 185	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B2RGM5 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: B3DF207837F3CCD9	FASTA	473	53,362
	10 20 30 40 50 60 MNYHSTNVNE IWDACLRILQ DIVDERAYRT WFLPIIPVSI EGDTLTLQVP SQFFCEFLEG 70 80 90 100 110 120 NFVEQLRTVL GRVIGPNASL QYNALVDNSS PKYPGTVTLA GCADGGQAAE QFDVNLLHRH 130 140 150 160 170 180 MPNAATHSEA QDFDTQLNSR LNFRNFYQSE CNYVARSVAE AIAASPGNTP MNPFFIYGAS 190 200 210 220 230 240 GVGKTHLCHA LGLRVREMHP RLKVLYVSSH LFEMQFTTAA RMGTINDFIA FYQQVDVLII 250 260 270 280 290 300 DDIQWLIGKK KTQLAFFQVF NHLYMLGKQI VLTSDKPPVD LNGMEERLVT RMAGATCVKI 310 320 330 340 350 360 ERPDLKLRRE ILQQRTLQSG VRLDESVLNF IAENVCDNVR ELEGTLVSLI TNSVVVGKEI 370 380 390 400 410 420 DLTFAKRIVR QAVRLEKKEV TIECIQQAVS RVFQVQIEQM KSKSRKQDIV QARQVVMFLS 430 440 450 460 470 KKHTAQSLSA IGELMGGRNH ATVLHGCRCV TNEMEMNASF RSSVERAEQL IAN

References

[1]

"Determination of the genome sequence of Porphyromonas gingivalis strain ATCC 33277 and genomic comparison with strain W83 revealed extensive genome rearrangements in P. gingivalis." Naito M., Hirakawa H., Yamashita A., Ohara N., Shoji M., Yukitake H., Nakayama K., Toh H., Yoshimura F., Kuhara S., Hattori M., Hayashi T., Nakayama K. DNA Res. 15:215-225(2008) [PubMed: 18524787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33277 / DSM 20709 / JCM 12257.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009380 Genomic DNA. Translation: BAG32520.1.
RefSeq	YP_001928117.1. NC_010729.1.
3D structure databases
ProteinModelPortal	B2RGM5.
ModBase	Search...
Protein-protein interaction databases
STRING	B2RGM5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6330508.
GenomeReviews	Gene locus PGN_0001 in contig AP009380_GR.
KEGG	pgn:PGN_0001.
PATRIC	22972913. VBIPorGin26334_0001.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG695666.
OMA	CHTLNAL.
ProtClustDB	PRK00149.
Family and domain databases
HAMAP	MF_00377. DnaA_bact. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Gene3D	G3DSA:1.10.1750.10. DnaA_C. 1 hit.
KO	K02313.
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_PORG3

Accession

Primary (citable) accession number: B2RGM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 1, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program