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Protein

Peptidase T

Gene

pepT

Organism
Escherichia coli O157:H7 str. EC4113
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves the N-terminal amino acid of tripeptides.UniRule annotation

Catalytic activityi

Release of the N-terminal residue from a tripeptide.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Zn2+UniRule annotationSAAS annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotationSAAS annotation
  • Zn2+Note: Binds 2 Zn2+ ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi78 – 781Zinc 1UniRule annotation
Active sitei80 – 801UniRule annotation
Metal bindingi140 – 1401Zinc 1UniRule annotation
Metal bindingi140 – 1401Zinc 2UniRule annotation
Active sitei173 – 1731Proton acceptorUniRule annotation
Metal bindingi174 – 1741Zinc 2UniRule annotation
Metal bindingi196 – 1961Zinc 1UniRule annotation
Metal bindingi379 – 3791Zinc 2UniRule annotation

GO - Molecular functioni

  1. metallopeptidase activity Source: UniProtKB-KW
  2. tripeptide aminopeptidase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. peptide catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

AminopeptidaseUniRule annotationSAAS annotation, Hydrolase, MetalloproteaseUniRule annotationSAAS annotation, Protease

Keywords - Ligandi

Metal-bindingUniRule annotation, ZincUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidase TUniRule annotationSAAS annotation (EC:3.4.11.4UniRule annotationSAAS annotation)
Alternative name(s):
AminotripeptidaseUniRule annotation
Tripeptide aminopeptidaseUniRule annotation
Gene namesi
Name:pepTUniRule annotationImported
ORF Names:ECH7EC4113_4569Imported
OrganismiEscherichia coli O157:H7 str. EC4113Imported
Taxonomic identifieri444452 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliB2P6S8.
SMRiB2P6S8. Positions 1-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20B family.UniRule annotation

Phylogenomic databases

OMAiYVYATIP.

Family and domain databases

Gene3Di3.30.70.360. 1 hit.
HAMAPiMF_00550. Aminopeptidase_M20.
InterProiIPR001261. ArgE/DapE_CS.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
IPR010161. Peptidase_M20B.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF037215. Peptidase_M20B. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01882. peptidase-T. 1 hit.
PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2P6S8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKLLERFLN YVSLDTQSKA GVRQVPSTEG QWKLLHLLKE QLEEMGLINV
60 70 80 90 100
TLSEKGTLMA TLPANVPGDI PAIGFISHVD TSPDCSGKNV NPQIVENYRG
110 120 130 140 150
GDIALGIGDE VLSPVMFPVL HQLLGQTLIT TDGKTLLGAD DKAGIAEIMT
160 170 180 190 200
ALAVLQQKNI PHGDIRVAFT PDEEVGKGAK HFDVDAFDAR WAYTVDGGGV
210 220 230 240 250
GELEFENFNA ASVNIKIVGN NVHPGTAKGV MVNALSLAAR IHAEVPADES
260 270 280 290 300
PEMTEGYEGF YHLASMKGTV ERADMHYIIR DFDRKQFEAR KRKMMEIAKK
310 320 330 340 350
VGKGLHPDCY IELVIEDSYY NMREKVVEHP HILDIAQQAM RDCDIEPELK
360 370 380 390 400
PIRGGTDGAQ LSFMGLPCPN LFTGGYNYHG KHEFVTLEGM EKAVQVIVRI

AELTAQRK
Length:408
Mass (Da):44,909
Last modified:July 1, 2008 - v1
Checksum:i35744B1DF4CC1DA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ABHP01000017 Genomic DNA. Translation: EDU54147.1.

Genome annotation databases

EnsemblBacteriaiEDU54147; EDU54147; ECH7EC4113_4569.
PATRICi26598921. VBIEscCol134660_2699.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ABHP01000017 Genomic DNA. Translation: EDU54147.1.

3D structure databases

ProteinModelPortaliB2P6S8.
SMRiB2P6S8. Positions 1-408.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEDU54147; EDU54147; ECH7EC4113_4569.
PATRICi26598921. VBIEscCol134660_2699.

Phylogenomic databases

OMAiYVYATIP.

Family and domain databases

Gene3Di3.30.70.360. 1 hit.
HAMAPiMF_00550. Aminopeptidase_M20.
InterProiIPR001261. ArgE/DapE_CS.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
IPR010161. Peptidase_M20B.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFiPIRSF037215. Peptidase_M20B. 1 hit.
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01882. peptidase-T. 1 hit.
PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Rosovitz M.J., Ravel J.
    Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: EC4113Imported.
  2. "Annotation of Escherichia coli O157:H7 str. EC4113."
    Eppinger M., Ravel J., Mammel M.K., LeClerc J.E., Cebula T.A., Sebastian Y.
    Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: EC4113Imported.

Entry informationi

Entry nameiB2P6S8_ECO57
AccessioniPrimary (citable) accession number: B2P6S8
Entry historyi
Integrated into UniProtKB/TrEMBL: July 1, 2008
Last sequence update: July 1, 2008
Last modified: March 4, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.