Reviewed, UniProtKB/Swiss-Prot B2MW51 (L_PARVP)

Names and origin

Protein names

Recommended name:
    RNA-directed RNA polymerase
    EC=2.7.7.48
Alternative name(s):
    Protein L

Gene names

Name:

L

Organism

Parana virus (isolate Rat/Paraguay/12056/1965) (PARV)

Taxonomic identifier

45222 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › New world arenaviruses

Virus host

Oryzomys angouya (Paraguayan rice rat) (Oryzomys buccinatus) [TaxID: 530180]

Protein attributes

Sequence length	2202 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2202	2202	RNA-directed RNA polymerase		PRO_0000361644
Regions
	Domain	1158 – 1353	196	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	B2MW51-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: B3F2709E75D5C991	FASTA	2,202	252,553
	10 20 30 40 50 60 MTEIISELKD LIRKWVPDEE HYIEQKSLTL SQVHTRAVVI EGLKLLSLIV EVDSCLKHKC 70 80 90 100 110 120 IPNRNKTVNM ILKDHKLVGP TLPECTPDGY YLCGDVLILL EVFVRSNQSA FEKKYAADFE 130 140 150 160 170 180 KLMSLAKDLT SYGITLVPVI DGRSNYYVEA IPDWVIERLR WLLLKVMDSL KDEGEDIEET 190 200 210 220 230 240 EYSRLIHSLS TMENQNLGLE SISQLKQTGL TYKKKLQALF TKNIRPNMSL GECRLKLIEI 250 260 270 280 290 300 FNEFRLRLES GTVERAYTQT NQDFLLNKLK EHSLLKVSRV SKFDSKGDCH LCSNHLIKVL 310 320 330 340 350 360 GLLKRQTTDE KEAPRIGMIR REYGLILSTC NKIKGQKILN TRRNTLLSLD VVMFNAFLSL 370 380 390 400 410 420 LRTYGEIVFD LMVGGCLQSV NDRLVSPSLI IDLYNKKCTR NPQWLSKVML KLNLLPGYIQ 430 440 450 460 470 480 EDFKVFVRPH LVELDLDLWS NYLELYSTTF ERRLEIQYTV SEEEVHEKTD PEVIEWLEMK 490 500 510 520 530 540 ESTFKQYLDS LSTLSLGLVN SMKTSGTSRF HINQPNDYYG TVKCEECFFQ SLHHAYGVSL 550 560 570 580 590 600 LYQKTGERNR CYSLCSNSGK VGHIVSFYCD PKRFFLPIMS QQVLLSMTQE MLSWLDFIKG 610 620 630 640 650 660 DDLNLISSML RRLILSVLAC PSKRVQLYLQ GLRYFLMAYV NEIHHVQLLA KLEVEVKSRS 670 680 690 700 710 720 EWATMTLADD LVVALLNMSN QPNMSKTFKF LLNVSYLCHL ITKETPDRLT DQIKCFEKFM 730 740 750 760 770 780 EPKIDFGSVI VNSSLNGNMT EEEESKILLD IDRLLSKQLN TSDQISKPGV NPTVMSLCCS 790 800 810 820 830 840 LFNLGELDVN GKLKRDPQSP SFTSTALDMS SNKSVVVPKL DELGNPLSTY DYAAVVSSVI 850 860 870 880 890 900 VDLSEGFKNK LRYKLDPHTL KYKIYKRFLS LVSDKTPKEN KLEMSQDEFL DEITDEQLEM 910 920 930 940 950 960 IEKIEMEVNE CLSKAANVEP RIGTGPDDKN PLKSLWAKEI LCVIESETSK HEVKDFDYTL 970 980 990 1000 1010 1020 FHHETYKELV ELVFNSNSRN QYFTDRILNP CPLEYLMKNL CRKYYEEEDY FECFKYILVS 1030 1040 1050 1060 1070 1080 TGFDNKVGRY DHKNMCRLGF KQSATRIRED ARISCRESNS ESILKRLDKS FFTNSSLRNL 1090 1100 1110 1120 1130 1140 CFYSEESPTA FDSVSSDLGR LKFGLSYKEQ VGGNRELYVG DLNTKLMTRL IEDYFECLME 1150 1160 1170 1180 1190 1200 KMKYTCLNNE AEFERALLDM KSVVRRSGFT VSMDHSKWGP HMSPVIFSQL FKALQFSLPD 1210 1220 1230 1240 1250 1260 GSQIDKEPIL NLLSWHIHKV VEVPFNVVHA YTKGYLKRLC GLMDNRTSKT EDFMDKFFAD 1270 1280 1290 1300 1310 1320 EVIPSHISSV LDMGQGILHN VSDFYGLITE QFISYALQTC VGVLSMAFTS SDDEILLGVT 1330 1340 1350 1360 1370 1380 NDLKNQDESL DIDKSLEFLE FHNYLSATLN KFISPKTVAG TFACEFKSRF YIWSQEVPLL 1390 1400 1410 1420 1430 1440 TKFTAAALHN VKAKAPNQLC ETIDTIQDQC VANGVSVEVV GAISKRTNNI IKYSGHPSNP 1450 1460 1470 1480 1490 1500 FLCLNDMDVK DWVDGSRGYR LQRSVENVYN DDEIPEYVRD MAARLFYLIR NGQVQEEYLI 1510 1520 1530 1540 1550 1560 SSMQSDPDEC FTLLAGILGV PEQNISRLLD IRWLNLRAHG DLRMVLRTKL MGSNRVIQRE 1570 1580 1590 1600 1610 1620 EVPSLIKSVQ SKLSKNFVRG ARKIITDAVN KSAFQSCIAA GFVGICKSMG SKCVRDGKGG 1630 1640 1650 1660 1670 1680 FMYIKDILSK IQYHKNCDVC KVSSGIYCKE SLKAVSDFSR PLFWDYFALV LTNACELGNW 1690 1700 1710 1720 1730 1740 VFSKPKIPDV VYKLDNPNFF WPVKPASHTE LEDKIGMNHV LYSIKRNYPD IFEEHLAPYL 1750 1760 1770 1780 1790 1800 TDLNTLKLSW VQKVKFLDIC VAIDMVSESL GIISHMIKRK REELYIVKQN EQSMSHTRES 1810 1820 1830 1840 1850 1860 QELAGGFRVT NEQICHNFLL QILFDSMITP VLLTSSQFKK YFWYGEVELL PNDCEHPLGQ 1870 1880 1890 1900 1910 1920 LTQFIMDCKK LNLSRAMNLD DLDVGFVHST IKLSDVFLNF STFLTKVDWE NRKDYNNLEE 1930 1940 1950 1960 1970 1980 LLKSTLESQL VLSIGLTFTH LRRSLKYKYE RSTVYTIIAK VILDIEQLTL NEDDQICLIV 1990 2000 2010 2020 2030 2040 QEVECYVSQS GGDHISLDGA ALIPLTPIIS GTETLCLDEV AIRQDDMLKG VSKHLGNVKL 2050 2060 2070 2080 2090 2100 DFSSHIKELK NKFSYKIQGP QVGMNPLHID KGIIMEGDKV VSRLDVNVTA KSLFMALELL 2110 2120 2130 2140 2150 2160 TDDELIRKFL RSLFYYLKSV KKGTALISLL SSDLKDIAEV YFNHFKDILK EEADWVSFGS 2170 2180 2190 2200 FQLVYSKSLD TIMIGDEKGE FRLKGVNCKR LIPVIPAVQE IE

References

[1]	"Phylogeny of the genus Arenavirus." Charrel R.N., de Lamballerie X., Emonet S. Curr. Opin. Microbiol. 11:362-368(2008) [PubMed: 18602020] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	EU627613 Genomic RNA. Translation: ACC94302.1.
RefSeq	YP_001936028.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6334530.
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_PARVP

Accession

Primary (citable) accession number: B2MW51

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	June 10, 2008
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)