B2MW50 (Z_PARVP) Reviewed, UniProtKB/Swiss-Prot
Last modified April 3, 2013. Version 28. History...
Names and origin
|Protein names||Recommended name:|
RING finger protein Z
Short name=Protein Z
|Organism||Parana virus (isolate Rat/Paraguay/12056/1965) (PARV) [Complete proteome]|
|Taxonomic identifier||45222 [NCBI]|
|Taxonomic lineage||Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › New world arenaviruses|
|Virus host||Oryzomys angouya (Paraguayan rice rat) (Oryzomys buccinatus) [TaxID: 530180]|
|Sequence length||95 AA.|
|Sequence processing||The displayed sequence is further processed into a mature form.|
|Protein existence||Inferred from homology|
General annotation (Comments)
Plays a crucial role in virion assembly and budding. Expressed late in the virus life cycle, it acts as an inhibitor of viral transcription and RNA synthesis by interacting with the viral polymerase L. Presumably recruits the NP encapsidated genome to cellular membranes at budding sites via direct interaction with NP. The budding of the virus progeny occurs after association of protein Z with the viral glycoprotein complex SSP-GP1-GP2 at the cell periphery, step that requires myristoylation of protein Z By similarity.
Interacts with protein N; this interaction probably directs the encapsidated genome to budding sites. Interacts (via RING domain) with polymerase L; this interaction inhibits viral transcription and replication. Interacts with the glycoprotein complex; this interaction plays a role in virion budding.
Virion. Host cytoplasm › host perinuclear region. Host cell membrane; Lipid-anchor; Cytoplasmic side. Note: Mainly perinuclear. During budding, associates at the inner side of the plasma membrane of infected cells By similarity.
The RING finger domain is essential for the inhibitory activity of protein Z in transcription and RNA replication By similarity.
Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Ring-finger protein Z contains one L domain: a PSAP motif that may be sufficient to mediate budding By similarity.
Belongs to the arenaviridae Z protein family.
Contains 1 RING-type zinc finger.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Initiator methionine||1||1||Removed; by host By similarity|
|Chain||2 – 95||94||RING finger protein Z||PRO_0000361038|
|Zinc finger||38 – 74||37||RING-type; atypical|
|Motif||88 – 91||4||PTAP/PSAP motif|
Amino acid modifications
|Lipidation||2||1||N-myristoyl glycine; by host By similarity|
|EU627613 Genomic RNA. Translation: ACC94301.1.|
|RefSeq||YP_001936027.1. NC_010761.1. |
3D structure databases
Protocols and materials databases
Genome annotation databases
Family and domain databases
|InterPro||IPR003224. Znf_P11. |
|Pfam||PF03854. zf-P11. 1 hit. |
|PROSITE||PS00518. ZF_RING_1. False negative. |
PS50089. ZF_RING_2. False negative.
|Accession||Primary (citable) accession number: B2MW50|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|
Index of protein domains and families