Reviewed, UniProtKB/Swiss-Prot B2MW49 (L_LATVB)

Names and origin

Protein names

Recommended name:
    RNA-directed RNA polymerase
    EC=2.7.7.48
Alternative name(s):
    Protein L

Gene names

Name:

L

Organism

Latino virus (isolate Rat/Bolivia/MARU 1924/1965) (LATV)

Taxonomic identifier

45221 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › New world arenaviruses

Virus host

Calomys callosus (Large vesper mouse) [TaxID: 56210]

Protein attributes

Sequence length	2238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2238	2238	RNA-directed RNA polymerase		PRO_0000361640
Regions
	Domain	1188 – 1387	200	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	B2MW49-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 218FA14B9576882D	FASTA	2,238	256,323
	10 20 30 40 50 60 MDDIVNQLFD LLRKHFPARP KVTEQITLVT CQNDAKMILT EGFKLLSLLV ELDSAEANNC 70 80 90 100 110 120 THNSDSLTIE GILRKEGIMS IALPRIVPDG FSLYGNVLIL LETFVRVNPV SFEQKYNQDM 130 140 150 160 170 180 SKLLSLKDDL SLCGITLVPL VDGRTNYYNR FVDDWVIERF RWLLLQLIKF VRESGEEIEE 190 200 210 220 230 240 LEYQRLITSL SKLENQSLGF ENIEKLPQTG LKYRDELKKH MFGNLSSKMK ESEIQENLIN 250 260 270 280 290 300 VLKEFFIKEY KNNKSLHKFV FTNRDGLLAK LDQITHHSEH PVDCMSCSSK LYSIIDKLGT 310 320 330 340 350 360 LKRQPLHSDY HPIYAKMWHS DSLSQAEQIY LKLLSQCNKI KSAKLLNTRR NTLLFLDLIM 370 380 390 400 410 420 VNFIVHSWKQ NPEVLTEYRR CGLMAGQLAL FSNDRYFDLN ELRNKLINKL KNCENWIAKC 430 440 450 460 470 480 VHQLKKQEFV ALDDVLVWAT VPDFESLELI TTSLELKRFK LQYGKDKVDH NEHPIGPLSE 490 500 510 520 530 540 ETFFRNLNVL SSVCLALVNS MKTSFTSKTV INERRASNHF GEVDLIECYC QRFFLSKDLV 550 560 570 580 590 600 GILSYQKTGE KSRCYSISLI SNGELEYIGS FYCDPKRFFL PIFSQIVLLN MSREMMLWLA 610 620 630 640 650 660 DLNLNDSLVG DKLRKLILLI VTNPSKRNQT FLQGLRYFIM AYVNQFHHVE LMDRLIVPVK 670 680 690 700 710 720 SYCESCLQRI SFDIFRLILE GDYDNEHMTR KFKFLLNVSY LCHLITKETP DRLTDQIKCF 730 740 750 760 770 780 EKFMEPKLKF ESVIVNPSLT ENMTEDEEAQ VLKGVDKLLG KSLSCSTDLT SPGVSKTLLS 790 800 810 820 830 840 MCVSSFNRGL LNVNGHLRQD PYRPNFTSTA LDLSSNKSVV VPKLDELGNP ISRYDYELLV 850 860 870 880 890 900 SSCVTNLAEG FKTKGKFKLD INCQEYTIMR NLTNLVLKNE DKSDAKIKGE KPCSFELSQW 910 920 930 940 950 960 METLSEEQLE VLEKLKGDVN IALGKLKEKG RSKSNSTLKE GVKRLDSGNT LAGCADPQQV 970 980 990 1000 1010 1020 LVNLWSEFGV MKQILVEVSL HEIKDFDPDI IPPQMIQKLV FKVNNSNYKS LFFLDSVINP 1030 1040 1050 1060 1070 1080 CPLELLIKNM TTATFDDGEL FECFKYLLIT AGFDQKLGTY EHKNRSRFGF KFEALKVREE 1090 1100 1110 1120 1130 1140 GRMSSRESNS EAIARRLDKS VFTNSALRNL CFYSDESPIS YSHVSPDVGK LKFGLSYKEQ 1150 1160 1170 1180 1190 1200 VGSNRELYVG DLNTKLMTRL IEDFSESVVS NMSYSCLNNE AEFEKAITDM KMCVNLGDMS 1210 1220 1230 1240 1250 1260 LSLDHSKWGP HMSPVIFAAF LQGLDLKYGP SLCKLNTDPI ITLLSWHIHK VVEVPYNVIH 1270 1280 1290 1300 1310 1320 AYVTGMIKRQ LGLMNMSGST ITESFVHRLL KEKREPLSHV MSVIDMGQGI LHNMSDLYGL 1330 1340 1350 1360 1370 1380 VTEQFINYAI HFLFDMNTTS YTSSDDQISM IKIGSGMCNF ESLKVIEEWE TILNFHAFIS 1390 1400 1410 1420 1430 1440 TKFNKFVSPK TVAGTFAAEF KSRFFVWGEE VPLLTKFVSA ALHNVKCKTP VQLSETVDTI 1450 1460 1470 1480 1490 1500 CDQCVANGVS VEIVSYICNR TNRLIRYSGF GEHPFLNVEN LDVKDWVDGS RGYRLQRNIE 1510 1520 1530 1540 1550 1560 LHLESDGCTS FIRQAARKVF SNIKSGKIVE QALVDLVQED GDKAITGFLR SVGVSEEDIA 1570 1580 1590 1600 1610 1620 LLCRIRWINL CAHGDLRLVL RTKLMSSRRI IETEEIPSLI KSIQSKLSKN FVKGAKKILA 1630 1640 1650 1660 1670 1680 ESINKSAFQS SIASGFIGFC QSVGSKCVRT GEGGFYYIKE LKSKVDLYCP CEVCARWKGV 1690 1700 1710 1720 1730 1740 TYCSSSCLKI ESFTRPLMWD YFSLVLSNAC ELGEWVFEDV EYPKDINFLR NPNLFWLVKP 1750 1760 1770 1780 1790 1800 RVSCQIEEKL GLTHILQSIR RNYPQLFETH LSPFMSDFQA GRTLGTMTVK FLDVCVALDL 1810 1820 1830 1840 1850 1860 ANENLGIVKH FLKNRRHDIY IVKQDESSQS HIRCQKSICV DVELTSTQVC QNFMTQLIMS 1870 1880 1890 1900 1910 1920 SLVQPLVLTS SELKKFNWFQ QVLTLETDED VDLGLLTDFA LQVKKFNVDR AMHSEDLSAG 1930 1940 1950 1960 1970 1980 YISSTVSVTT FSLSKPIFLQ QIDSDFIGGT EDRKDFIQMI KSEFTKNSID LQFVIQISHV 1990 2000 2010 2020 2030 2040 KRALRFNLKR TTVYTLIVRT SILKEVILNS LGQEDQSVEL VVDDLELFCS GHDGNHFTLD 2050 2060 2070 2080 2090 2100 AAPLIVQEPL INGNLKFDLV SRLEEEDLTF SYSESLPSFH FNFEKYKHEL CNKFSYHLSG 2110 2120 2130 2140 2150 2160 PVIVDEPLVL DRGVILHGGR KLTTLQFDFS ADRIMQALSE LESLSSRDLF LFNLWVYSDQ 2170 2180 2190 2200 2210 2220 TKSKLYIHQD KLLLLVESYL SELNSSLARY DSWLNLGNYM ICYSKSFKCL MISDTNGRNR 2230 LKGILCRRLI EEEVQDIE

References

[1]	"Phylogeny of the genus Arenavirus." Charrel R.N., de Lamballerie X., Emonet S. Curr. Opin. Microbiol. 11:362-368(2008) [PubMed: 18602020] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	EU627612 Genomic RNA. Translation: ACC94300.1.
RefSeq	YP_001936026.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6334524.
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_LATVB

Accession

Primary (citable) accession number: B2MW49

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	June 10, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)