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Protein

Carnocyclin-A

Gene

cclA

Organism
Carnobacterium maltaromaticum (Carnobacterium piscicola)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cyclopeptide antibiotic that inhibits the growth of Gram-positive bacteria, but has no effect on the growth of Gram-negative bacteria.1 Publication

pH dependencei

Stable from pH 2 to 12.1 Publication

Temperature dependencei

Displays a high degree of stability when incubated at temperatures between -80 and 75 degrees Celsius for 60 minutes. Autoclaving the peptide at 121 degrees Celsius for 15 minutes had no effect but incubation at 100 degrees Celsius for 60 minutes caused a 32-fold reduction in activity.1 Publication

GO - Biological processi

  • cytolysis Source: UniProtKB-KW
  • defense response to Gram-positive bacterium Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin

Protein family/group databases

TCDBi1.C.90.1.1. the carnocyclin a (carnocyclin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Carnocyclin-A
Gene namesi
Name:cclAImported
OrganismiCarnobacterium maltaromaticum (Carnobacterium piscicola)
Taxonomic identifieri2751 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesCarnobacteriaceaeCarnobacterium

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 441 PublicationPRO_0000361696
Peptidei5 – 6460Carnocyclin-APRO_0000361697Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki5 ↔ 64Cyclopeptide (Leu-Leu)1 Publication

Structurei

Secondary structure

1
64
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni6 – 94Combined sources
Helixi12 – 2211Combined sources
Turni23 – 253Combined sources
Helixi28 – 358Combined sources
Turni40 – 423Combined sources
Helixi43 – 5513Combined sources
Helixi58 – 636Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KJFNMR-A5-64[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiB2MVM5.

Family & Domainsi

Family and domain databases

InterProiIPR020038. Bacteriocin_uberolysin_fam.
[Graphical view]
PfamiPF09221. Bacteriocin_IId. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03651. circ_ocin_uber. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B2MVM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYELVAYGI AQGTAEKVVS LINAGLTVGS IISILGGVTV GLSGVFTAVK
60
AAIAKQGIKK AIQL
Length:64
Mass (Da):6,417
Last modified:June 10, 2008 - v1
Checksum:i141B4BEF7FE9E56C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321I → L AA sequence (PubMed:18552180).Curated

Mass spectrometryi

Molecular mass is 5862 Da from positions 5 - 64. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU624394 Genomic DNA. Translation: ACC93994.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU624394 Genomic DNA. Translation: ACC93994.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KJFNMR-A5-64[»]
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.C.90.1.1. the carnocyclin a (carnocyclin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiB2MVM5.

Family and domain databases

InterProiIPR020038. Bacteriocin_uberolysin_fam.
[Graphical view]
PfamiPF09221. Bacteriocin_IId. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03651. circ_ocin_uber. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of carnocyclin a, a novel circular bacteriocin produced by Carnobacterium maltaromaticum UAL307."
    Martin-Visscher L.A., van Belkum M.J., Garneau-Tsodikova S., Whittal R.M., Zheng J., McMullen L.M., Vederas J.C.
    Appl. Environ. Microbiol. 74:4756-4763(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 5-64, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
    Strain: UAL307Imported.

Entry informationi

Entry nameiCCLA_CARML
AccessioniPrimary (citable) accession number: B2MVM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: June 10, 2008
Last modified: October 29, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.