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Protein

Dextransucrase 1

Gene

dsrF

Organism
Leuconostoc mesenteroides
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Involved in the production of dextran, an extracellular glucan polymer.By similarity

Catalytic activityi

Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Protein family/group databases

CAZyiGH70. Glycoside Hydrolase Family 70.

Names & Taxonomyi

Protein namesi
Recommended name:
Dextransucrase 1 (EC:2.4.1.5)
Alternative name(s):
Glucansucrase 1
Sucrose 6-glucosyltransferase 1
Gene namesi
Name:dsrF
OrganismiLeuconostoc mesenteroides
Taxonomic identifieri1245 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeLeuconostoc

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›284›284Dextransucrase 1PRO_0000403441Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi203120.LEUM_1747.

Structurei

3D structure databases

ProteinModelPortaliB2MUU6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 70 family.Curated

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR003318. Glyco_hydro70cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02324. Glyco_hydro_70. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Fragment.

B2MUU6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
DTVPRVYYAD LYTDDGQYMA TKSPFFDAIN TLLKARVQYV AGGQSMSVDS
60 70 80 90 100
NDVLTSVRYG KNAMTASDTG TSETRTEGVG VIVSNNAELQ LEDGHTVTLH
110 120 130 140 150
MGAAHKNQAY RALLSTTADG LAYYDTDENA PVAYTDANGD LIFTNESIYG
160 170 180 190 200
VQNPQVSGYL AVWVPVGAQQ DQDARTASDT TTNTSDKVFH SNAALDSQVI
210 220 230 240 250
YEGFSNFQAF ATDSSEYTNV VIAQNADQFK QWGVTSFQLA PQYRSSTDTS
260 270 280
FLDSIIQNGY AFTDRYDLGY GTPTQYGAVD HVRA
Length:284
Mass (Da):30,936
Last modified:June 10, 2008 - v1
Checksum:iF6228D1BE8189CF0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei284 – 2841

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU621844 Genomic DNA. Translation: ACC65964.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU621844 Genomic DNA. Translation: ACC65964.1.

3D structure databases

ProteinModelPortaliB2MUU6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203120.LEUM_1747.

Protein family/group databases

CAZyiGH70. Glycoside Hydrolase Family 70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR003318. Glyco_hydro70cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02324. Glyco_hydro_70. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Sarwat F.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CMG713.
  2. "Production & characterization of a unique dextran from an indigenous Leuconostoc mesenteroides CMG713."
    Sarwat F., Ul Qader S.A., Aman A., Ahmed N.
    Int. J. Biol. Sci. 4:379-386(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERISTICS OF DEXTRAN.
    Strain: CMG713.

Entry informationi

Entry nameiGTF1_LEUME
AccessioniPrimary (citable) accession number: B2MUU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: June 10, 2008
Last modified: June 24, 2015
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The dextran produced by Leuconostoc mesenteroides CMG713 has a molecular mass of about 2000 kDa and contains exclusively alpha-(1->6) glycosidic linkages in the main chain.

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.