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Protein

UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase

Gene

murF

Organism
Elusimicrobium minutum (strain Pei191)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationImported
Biological processCell cycle, Cell divisionUniRule annotation, Cell shape, Cell wall biogenesis/degradationUniRule annotation, Peptidoglycan synthesisUniRule annotation
LigandATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciEMIN445932:G1GBV-1282-MONOMER
UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseUniRule annotation (EC:6.3.2.10UniRule annotation)
Alternative name(s):
D-alanyl-D-alanine-adding enzymeUniRule annotation
Gene namesi
Name:murFUniRule annotation
Ordered Locus Names:Emin_1253Imported
OrganismiElusimicrobium minutum (strain Pei191)Imported
Taxonomic identifieri445932 [NCBI]
Taxonomic lineageiBacteriaElusimicrobiaElusimicrobiaElusimicrobialesElusimicrobiaceaeElusimicrobium
Proteomesi
  • UP000001029 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi445932.Emin_1253

Structurei

3D structure databases

ProteinModelPortaliB2KE57
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 103Mur_ligaseInterPro annotationAdd BLAST72
Domaini113 – 290Mur_ligase_MInterPro annotationAdd BLAST178
Domaini313 – 388Mur_ligase_CInterPro annotationAdd BLAST76

Sequence similaritiesi

Belongs to the MurCDEF family. MurF subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107EES Bacteria
COG0770 LUCA
HOGENOMiHOG000268120
KOiK01929
OMAiSYNNHWG
OrthoDBiPOG091H00FE

Family and domain databases

Gene3Di3.40.1190.10, 1 hit
3.90.190.20, 1 hit
HAMAPiMF_02019 MurF, 1 hit
InterProiView protein in InterPro
IPR036565 Mur-like_cat_sf
IPR004101 Mur_ligase_C
IPR036615 Mur_ligase_C_dom_sf
IPR013221 Mur_ligase_cen
IPR000713 Mur_ligase_N
IPR035911 MurE/MurF_N
IPR005863 UDP-N-AcMur_synth
PfamiView protein in Pfam
PF01225 Mur_ligase, 1 hit
PF02875 Mur_ligase_C, 1 hit
PF08245 Mur_ligase_M, 1 hit
SUPFAMiSSF53244 SSF53244, 1 hit
SSF53623 SSF53623, 1 hit
SSF63418 SSF63418, 1 hit
TIGRFAMsiTIGR01143 murF, 1 hit

Sequencei

Sequence statusi: Complete.

B2KE57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYLNLTLGSF AKIVDGRIIQ GNPLMPFNAF ITDSRKLTKG DIFWALKGEK
60 70 80 90 100
FDGNTFLQNA VDAGASALVA DKFFADKIDL KNTALVEVEN TLTALQTLAA
110 120 130 140 150
YHRRRSDLKV VAITGSNGKS TTKQMLLSIC KTSGPARANM GNLNNQFGLP
160 170 180 190 200
FSLLEIDSKD KFGVFELGAS KKGDILEIGK PTLPDVAIIT NVSAAHLQFF
210 220 230 240 250
KDLKTVYETK TELIDCLNFG GILVFNSDDA MLKKLKTEYK GKAISFGFKS
260 270 280 290 300
GADLQIKNKK NLEFVYKGEV FSFDLQFEKH NKLNAAAAAA GAIALGLYKD
310 320 330 340 350
NIEKGLAAYE AMPMRMEERK IGNVDFILDC YNANPASMKN AITLLAKRKA
360 370 380 390 400
GPRVAVLGDM KELGNCSAKY HAHLAKILIG KQIDYIFLSG PEMAAAAEAL
410 420 430 440 450
KTKNPAAKLK YSLEYKDWAQ DLKEVLKNGG TCLIKASRSM NYEKILDCLK
Length:450
Mass (Da):49,366
Last modified:June 10, 2008 - v1
Checksum:i1795587F1ACCE68E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001055 Genomic DNA Translation: ACC98803.1
RefSeqiWP_012415418.1, NC_010644.1

Genome annotation databases

EnsemblBacteriaiACC98803; ACC98803; Emin_1253
KEGGiemi:Emin_1253

Entry informationi

Entry nameiB2KE57_ELUMP
AccessioniPrimary (citable) accession number: B2KE57
Entry historyiIntegrated into UniProtKB/TrEMBL: June 10, 2008
Last sequence update: June 10, 2008
Last modified: March 28, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health