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B2KCN5 (PYRC_ELUMP) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydroorotase
Short name=DHOase
EC=3.5.2.3
Gene names
Name:pyrC
Ordered Locus Names:Emin_0726
OrganismElusimicrobium minutum (strain Pei191) [Complete proteome] [HAMAP]
Taxonomic identifier445932 [NCBI]
Taxonomic lineageBacteria › Elusimicrobia › Elusimicrobia (class) › Elusimicrobiales › Elusimicrobiaceae › Elusimicrobium

Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00220_B

Cofactor

Binds 2 zinc ions per subunit By similarity. HAMAP MF_00220_B

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00220_B

Subunit structure

Homodimer By similarity. HAMAP MF_00220_B

Sequence similarities

Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processpyrimidine nucleotide biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functiondihydroorotase activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 432432Dihydroorotase HAMAP MF_00220_B
PRO_1000100074

Sites

Metal binding601Zinc 1 By similarity
Metal binding621Zinc 1 By similarity
Metal binding1421Zinc 1; via carbamate group By similarity
Metal binding1421Zinc 2; via carbamate group By similarity
Metal binding1791Zinc 2 By similarity
Metal binding2321Zinc 2 By similarity
Metal binding3051Zinc 1 By similarity

Amino acid modifications

Modified residue1421N6-carboxylysine By similarity

Sequences

Sequence LengthMass (Da)Tools
B2KCN5 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 07F970F1DCEBDFB2

FASTA43246,430
        10         20         30         40         50         60 
MKYLIKNAHV IDPANKIDGL KDILIENGKI AAVENKIEDN AAKIIDAKGL TAMPGFVDMH 

        70         80         90        100        110        120 
THLREPGQEG KETIFTGTKA ALKGGFTTVC MMPNTNPAMD SKNNLAIAQD IIRKTANVNV 

       130        140        150        160        170        180 
EIMGAITKNR AGKELSNFAE LKQAGAIALS DDGSGVEDDA VMQAAFKESV KQDILLISHS 

       190        200        210        220        230        240 
EDSKLSAGGV MNEGLISTKL GLKPISNASE YEMVKREIQL AKGLDAKIHI AHVSTKESCE 

       250        260        270        280        290        300 
IIAKAKKQGV MVTAEATPHH FTLTDKACES FSGNTKMNPP LRSEADVEAL KQALKDGTID 

       310        320        330        340        350        360 
AIATDHAPHA VHEKEVEFDL AYFGIIGLET AFPLAYDVLV KSGLIDMAKL VGLMSLNPSK 

       370        380        390        400        410        420 
ILGLKKGTLT PGADADITIV DLNKQWVYSK EEVQSLSCNS PFIGKNLQGY IEYTFVGGEL 

       430 
KLENGTLKVK DA

« Hide

References

[1]"Genomic analysis of 'Elusimicrobium minutum,' the first cultivated representative of the phylum 'Elusimicrobia' (formerly termite group 1)."
Herlemann D.P.R., Geissinger O., Ikeda-Ohtsubo W., Kunin V., Sun H., Lapidus A., Hugenholtz P., Brune A.
Appl. Environ. Microbiol. 75:2841-2849(2009) [PubMed: 19270133] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Pei191.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001055 Genomic DNA. Translation: ACC98281.1.
RefSeqYP_001875618.1. NC_010644.1.

3D structure databases

ProteinModelPortalB2KCN5.
ModBaseSearch...

Protein-protein interaction databases

STRINGB2KCN5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6263361.
GenomeReviewsGene locus Emin_0726 in contig CP001055_GR.
KEGGemi:Emin_0726.
PATRIC21848646. VBIEluMin86178_0758.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG724623.
OMANTPYESM.

Family and domain databases

HAMAPMF_00220_B. PyrC_type2_B.
[Tree]
InterProIPR006680. Amidohydro_1.
IPR004722. DHOase.
IPR002195. Dihydroorotase_CS.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
KOK01465.
PfamPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMSSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMsTIGR00857. PyrC_multi. 1 hit.
PROSITEPS00482. DIHYDROOROTASE_1. False negative.
PS00483. DIHYDROOROTASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRC_ELUMP
AccessionPrimary (citable) accession number: B2KCN5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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