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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Elusimicrobium minutum (strain Pei191)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei614 – 6141ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEMIN445932:GHM0-208-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Emin_0201
OrganismiElusimicrobium minutum (strain Pei191)
Taxonomic identifieri445932 [NCBI]
Taxonomic lineageiBacteriaElusimicrobiaElusimicrobiaElusimicrobialesElusimicrobiaceaeElusimicrobium
ProteomesiUP000001029: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 835835Leucine--tRNA ligasePRO_1000091316Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi445932.Emin_0201.

Structurei

3D structure databases

ProteinModelPortaliB2KB05.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5212"HIGH" regionAdd
BLAST
Motifi611 – 6155"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2KB05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEINFQEID KKQQKIWEDN NLFVTPRLPI NPKFYCLDMF PYPSGQGLHV
60 70 80 90 100
GHPEGYTASD ILVRYKKMKG FDVIHPMGWD AFGLPAENYA IATGVHPAIT
110 120 130 140 150
TKNNIDNFRR QIKSLGMAYD WTREIDTTDP NYYRWTQWIF LQIFKKGLAY
160 170 180 190 200
ESTVPINWCP SCKTGLANEE VFNGNCERCG TKIESKNIRQ WVLKITEYAD
210 220 230 240 250
RLLEDLEGLD WPESTLAMQR NWIGKSIGAE VDFEIDGHKE NLKVFTTRPD
260 270 280 290 300
TLFGATYMVV SPEHPILEQI TMPEQKAAVK SYQEEAAAKT DFERGDVNKK
310 320 330 340 350
TGVFTGAYAI NPVNGKKIQI WTSDYVLMGY GTGAIMAVPA HDERDYEFAK
360 370 380 390 400
KFGLEIIEVI KSDKGVEKEA FTGDGELVNS DFLNGMKVEQ SKAAMIKFLE
410 420 430 440 450
EKGVGGAKTT YRLRDWVFSR QRYWGEPIPI VHCPKCGVVP VPESELPVKL
460 470 480 490 500
PEVTNYEPTG TGESPLANVP EWVNTVCPVC AGPAKRETNT MPQWAGSCWY
510 520 530 540 550
YLRYLDPKND KTFVNPEIER ECDPVDCYIG GAEHAVLHLL YSRFWHKVLY
560 570 580 590 600
DLGYVKYKEP YAKLRHQGMI LAYSYRGEDG VYHGYDEVDL SDLQNPKLKT
610 620 630 640 650
TGEKLNSMVE KMSKSKKNVI NPDDILHKYG ADAFRMYEMF MGPFEASKPW
660 670 680 690 700
DMKGIEGVNR FLKRVYAWGE SVETDEVFLS TKELDILRSK TIVKVSEDIE
710 720 730 740 750
KFNFNTAVSA LMIYFNDLSK IKGVPLKHFK TFLALLHPFA PHITEELWSR
760 770 780 790 800
RKCGPFLVKT AWPEADIMLL RSEDMGLGIQ VNGKVRGSIT VNASASDDEI
810 820 830
KAKAFEEPNV KKHMEGKALV KVIIVPKRMV NIVVK
Length:835
Mass (Da):95,166
Last modified:June 10, 2008 - v1
Checksum:i955DAACAC38CCF7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001055 Genomic DNA. Translation: ACC97764.1.
RefSeqiWP_012414379.1. NC_010644.1.
YP_001875101.1. NC_010644.1.

Genome annotation databases

EnsemblBacteriaiACC97764; ACC97764; Emin_0201.
GeneIDi6262872.
KEGGiemi:Emin_0201.
PATRICi21847506. VBIEluMin86178_0207.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001055 Genomic DNA. Translation: ACC97764.1.
RefSeqiWP_012414379.1. NC_010644.1.
YP_001875101.1. NC_010644.1.

3D structure databases

ProteinModelPortaliB2KB05.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi445932.Emin_0201.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC97764; ACC97764; Emin_0201.
GeneIDi6262872.
KEGGiemi:Emin_0201.
PATRICi21847506. VBIEluMin86178_0207.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciEMIN445932:GHM0-208-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genomic analysis of 'Elusimicrobium minutum,' the first cultivated representative of the phylum 'Elusimicrobia' (formerly termite group 1)."
    Herlemann D.P.R., Geissinger O., Ikeda-Ohtsubo W., Kunin V., Sun H., Lapidus A., Hugenholtz P., Brune A.
    Appl. Environ. Microbiol. 75:2841-2849(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pei191.

Entry informationi

Entry nameiSYL_ELUMP
AccessioniPrimary (citable) accession number: B2KB05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: March 4, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.