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B2KB05 (SYL_ELUMP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Emin_0201
OrganismElusimicrobium minutum (strain Pei191) [Complete proteome] [HAMAP]
Taxonomic identifier445932 [NCBI]
Taxonomic lineageBacteriaElusimicrobiaElusimicrobiaElusimicrobialesElusimicrobiaceaeElusimicrobium

Protein attributes

Sequence length835 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 835835Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091316

Regions

Motif41 – 5212"HIGH" region HAMAP-Rule MF_00049
Motif611 – 6155"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6141ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B2KB05 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 955DAACAC38CCF7C

FASTA83595,166
        10         20         30         40         50         60 
MTEINFQEID KKQQKIWEDN NLFVTPRLPI NPKFYCLDMF PYPSGQGLHV GHPEGYTASD 

        70         80         90        100        110        120 
ILVRYKKMKG FDVIHPMGWD AFGLPAENYA IATGVHPAIT TKNNIDNFRR QIKSLGMAYD 

       130        140        150        160        170        180 
WTREIDTTDP NYYRWTQWIF LQIFKKGLAY ESTVPINWCP SCKTGLANEE VFNGNCERCG 

       190        200        210        220        230        240 
TKIESKNIRQ WVLKITEYAD RLLEDLEGLD WPESTLAMQR NWIGKSIGAE VDFEIDGHKE 

       250        260        270        280        290        300 
NLKVFTTRPD TLFGATYMVV SPEHPILEQI TMPEQKAAVK SYQEEAAAKT DFERGDVNKK 

       310        320        330        340        350        360 
TGVFTGAYAI NPVNGKKIQI WTSDYVLMGY GTGAIMAVPA HDERDYEFAK KFGLEIIEVI 

       370        380        390        400        410        420 
KSDKGVEKEA FTGDGELVNS DFLNGMKVEQ SKAAMIKFLE EKGVGGAKTT YRLRDWVFSR 

       430        440        450        460        470        480 
QRYWGEPIPI VHCPKCGVVP VPESELPVKL PEVTNYEPTG TGESPLANVP EWVNTVCPVC 

       490        500        510        520        530        540 
AGPAKRETNT MPQWAGSCWY YLRYLDPKND KTFVNPEIER ECDPVDCYIG GAEHAVLHLL 

       550        560        570        580        590        600 
YSRFWHKVLY DLGYVKYKEP YAKLRHQGMI LAYSYRGEDG VYHGYDEVDL SDLQNPKLKT 

       610        620        630        640        650        660 
TGEKLNSMVE KMSKSKKNVI NPDDILHKYG ADAFRMYEMF MGPFEASKPW DMKGIEGVNR 

       670        680        690        700        710        720 
FLKRVYAWGE SVETDEVFLS TKELDILRSK TIVKVSEDIE KFNFNTAVSA LMIYFNDLSK 

       730        740        750        760        770        780 
IKGVPLKHFK TFLALLHPFA PHITEELWSR RKCGPFLVKT AWPEADIMLL RSEDMGLGIQ 

       790        800        810        820        830 
VNGKVRGSIT VNASASDDEI KAKAFEEPNV KKHMEGKALV KVIIVPKRMV NIVVK 

« Hide

References

[1]"Genomic analysis of 'Elusimicrobium minutum,' the first cultivated representative of the phylum 'Elusimicrobia' (formerly termite group 1)."
Herlemann D.P.R., Geissinger O., Ikeda-Ohtsubo W., Kunin V., Sun H., Lapidus A., Hugenholtz P., Brune A.
Appl. Environ. Microbiol. 75:2841-2849(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Pei191.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001055 Genomic DNA. Translation: ACC97764.1.
RefSeqYP_001875101.1. NC_010644.1.

3D structure databases

ProteinModelPortalB2KB05.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING445932.Emin_0201.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACC97764; ACC97764; Emin_0201.
GeneID6262872.
KEGGemi:Emin_0201.
PATRIC21847506. VBIEluMin86178_0207.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAVNIVTND.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycEMIN445932:GHM0-208-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ELUMP
AccessionPrimary (citable) accession number: B2KB05
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries