B2KAV3 (SYE_ELUMP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Emin_0083

Organism

Elusimicrobium minutum (strain Pei191) [Complete proteome] [HAMAP]

Taxonomic identifier

445932 [NCBI]

Taxonomic lineage

Bacteria › Elusimicrobia › Elusimicrobia (class) › Elusimicrobiales › Elusimicrobiaceae › Elusimicrobium

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000367667
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022_B
	Motif	256 – 260	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Metal binding	112	1	Zinc By similarity
	Metal binding	114	1	Zinc By similarity
	Metal binding	139	1	Zinc By similarity
	Metal binding	141	1	Zinc By similarity
	Binding site	259	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2KAV3 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: ADF084EAF76A5D6F	FASTA	488	55,376
	10 20 30 40 50 60 MKIRVRFAPS PTGFLHIGGV RTALFNYLFA KRYGGTFVLR IEDTDELRST EESTQAIFDG 70 80 90 100 110 120 LEWTKLLWDE GPFRDGKENG PYPPYLQSER VKAGIYQKYI DQLLEEGKAY KCYCTPEELE 130 140 150 160 170 180 AMREEAAAKK LPPRYPGKCK HLTKDEQAAL EAQGRKPVIR FNMPSEGSVE WADLIRGPVS 190 200 210 220 230 240 FASKDLYDLV ISKPSGFPTY NFACVIDDHL MEMSHIIRGE DHISNTPMQI QMYKAFGWTP 250 260 270 280 290 300 PEFGHLPMIH GSDGTKLSKR HGATNVIEYQ KQGYLSEALV NYLALLGWSN SESQQLFAPG 310 320 330 340 350 360 ELEQKFDIKG VQKSPAIFDN AKLDWMNSEY IRATPISKLT DLAIPFIKEE NIDISKTDRA 370 380 390 400 410 420 ALENIIAIEQ EKYRTLKEIP GLIKFFFEDV VFEEGAKEKV YGKPESKDVL LGITRVYQNI 430 440 450 460 470 480 EPFKEADLEA ATRAFAKDNG FKTGQIFHPV RVAVSGRTHG PTLFKMLELL GKETVIKRLN EAAKYSNI

References

[1]

"Genomic analysis of 'Elusimicrobium minutum,' the first cultivated representative of the phylum 'Elusimicrobia' (formerly termite group 1)." Herlemann D.P.R., Geissinger O., Ikeda-Ohtsubo W., Kunin V., Sun H., Lapidus A., Hugenholtz P., Brune A. Appl. Environ. Microbiol. 75:2841-2849(2009) [PubMed: 19270133] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pei191.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001055 Genomic DNA. Translation: ACC97649.1.
RefSeq	YP_001874986.1. NC_010644.1.
3D structure databases
ProteinModelPortal	B2KAV3.
ModBase	Search...
Protein-protein interaction databases
STRING	B2KAV3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6263768.
GenomeReviews	Gene locus Emin_0083 in contig CP001055_GR.
KEGG	emi:Emin_0083.
PATRIC	21847252. VBIEluMin86178_0085.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	DKETAND.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_ELUMP

Accession

Primary (citable) accession number: B2KAV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program