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B2KAV3

- SYE_ELUMP

UniProt

B2KAV3 - SYE_ELUMP

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Elusimicrobium minutum (strain Pei191)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi112 – 1121ZincUniRule annotation
    Metal bindingi114 – 1141ZincUniRule annotation
    Metal bindingi139 – 1391ZincUniRule annotation
    Metal bindingi141 – 1411ZincUniRule annotation
    Binding sitei259 – 2591ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW
    4. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciEMIN445932:GHM0-84-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:Emin_0083
    OrganismiElusimicrobium minutum (strain Pei191)
    Taxonomic identifieri445932 [NCBI]
    Taxonomic lineageiBacteriaElusimicrobiaElusimicrobiaElusimicrobialesElusimicrobiaceaeElusimicrobium
    ProteomesiUP000001029: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 488488Glutamate--tRNA ligasePRO_0000367667Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi445932.Emin_0083.

    Structurei

    3D structure databases

    ProteinModelPortaliB2KAV3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi9 – 1911"HIGH" regionAdd
    BLAST
    Motifi256 – 2605"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252720.
    KOiK01885.
    OMAiWLPEEMG.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2KAV3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIRVRFAPS PTGFLHIGGV RTALFNYLFA KRYGGTFVLR IEDTDELRST    50
    EESTQAIFDG LEWTKLLWDE GPFRDGKENG PYPPYLQSER VKAGIYQKYI 100
    DQLLEEGKAY KCYCTPEELE AMREEAAAKK LPPRYPGKCK HLTKDEQAAL 150
    EAQGRKPVIR FNMPSEGSVE WADLIRGPVS FASKDLYDLV ISKPSGFPTY 200
    NFACVIDDHL MEMSHIIRGE DHISNTPMQI QMYKAFGWTP PEFGHLPMIH 250
    GSDGTKLSKR HGATNVIEYQ KQGYLSEALV NYLALLGWSN SESQQLFAPG 300
    ELEQKFDIKG VQKSPAIFDN AKLDWMNSEY IRATPISKLT DLAIPFIKEE 350
    NIDISKTDRA ALENIIAIEQ EKYRTLKEIP GLIKFFFEDV VFEEGAKEKV 400
    YGKPESKDVL LGITRVYQNI EPFKEADLEA ATRAFAKDNG FKTGQIFHPV 450
    RVAVSGRTHG PTLFKMLELL GKETVIKRLN EAAKYSNI 488
    Length:488
    Mass (Da):55,376
    Last modified:June 10, 2008 - v1
    Checksum:iADF084EAF76A5D6F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001055 Genomic DNA. Translation: ACC97649.1.
    RefSeqiYP_001874986.1. NC_010644.1.

    Genome annotation databases

    EnsemblBacteriaiACC97649; ACC97649; Emin_0083.
    GeneIDi6263768.
    KEGGiemi:Emin_0083.
    PATRICi21847252. VBIEluMin86178_0085.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001055 Genomic DNA. Translation: ACC97649.1 .
    RefSeqi YP_001874986.1. NC_010644.1.

    3D structure databases

    ProteinModelPortali B2KAV3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 445932.Emin_0083.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACC97649 ; ACC97649 ; Emin_0083 .
    GeneIDi 6263768.
    KEGGi emi:Emin_0083.
    PATRICi 21847252. VBIEluMin86178_0085.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252720.
    KOi K01885.
    OMAi WLPEEMG.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci EMIN445932:GHM0-84-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic analysis of 'Elusimicrobium minutum,' the first cultivated representative of the phylum 'Elusimicrobia' (formerly termite group 1)."
      Herlemann D.P.R., Geissinger O., Ikeda-Ohtsubo W., Kunin V., Sun H., Lapidus A., Hugenholtz P., Brune A.
      Appl. Environ. Microbiol. 75:2841-2849(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pei191.

    Entry informationi

    Entry nameiSYE_ELUMP
    AccessioniPrimary (citable) accession number: B2KAV3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3