Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tetraacyldisaccharide 4'-kinase

Gene

lpxK

Organism
Yersinia pseudotuberculosis serotype IB (strain PB1/+)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).UniRule annotation

Catalytic activityi

ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate).UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. no protein annotated in this organism
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi55 – 628ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYPSE502801:GHIH-1642-MONOMER.
UniPathwayiUPA00359; UER00482.

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraacyldisaccharide 4'-kinaseUniRule annotation (EC:2.7.1.130UniRule annotation)
Alternative name(s):
Lipid A 4'-kinaseUniRule annotation
Gene namesi
Name:lpxKUniRule annotation
Ordered Locus Names:YPTS_1523
OrganismiYersinia pseudotuberculosis serotype IB (strain PB1/+)
Taxonomic identifieri502801 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Tetraacyldisaccharide 4'-kinasePRO_1000123754Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB2KA30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxK family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000004953.
KOiK00912.
OMAiPTVIYLI.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00409. LpxK. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR003758. Tetraacyldisaccharide_4-kinase.
[Graphical view]
PfamiPF02606. LpxK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00682. lpxK. 1 hit.

Sequencei

Sequence statusi: Complete.

B2KA30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIERIWSGQS RLYLLLLPLS WLYGAVTWLI RASYRLGLRS AWRSPVPVII
60 70 80 90 100
VGNLTAGGNG KTPVVIWLVE QLQQRGYRVG VVSRGYGGKS AVYPLLLSDN
110 120 130 140 150
TTTAQAGDEP VLIFQRTGAP VAVSPKRADA IKALLQSHAV DFIITDDGLQ
160 170 180 190 200
HYALQRDFEL VVIDGVRRFG NGWWLPAGPM REREGRLRSV DAAITNGGLA
210 220 230 240 250
AEGEIPMQLV AREAVNLVTG QRQPAEQLQH VVAMAGIGHP PRFFATLNLL
260 270 280 290 300
GIKPENEHAF ADHQDYSLAQ LSRLTSGPQI LLMTEKDAVK CRAFALPNWW
310 320
YLPVDAQLPS DRADKLLLNI QALSPDTK
Length:328
Mass (Da):36,139
Last modified:June 10, 2008 - v1
Checksum:i46E9672BC2624CCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001048 Genomic DNA. Translation: ACC88495.1.
RefSeqiWP_002211319.1. NZ_CP009780.1.

Genome annotation databases

EnsemblBacteriaiACC88495; ACC88495; YPTS_1523.
KEGGiypb:YPTS_1523.
PATRICi18650864. VBIYerPse20639_1647.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001048 Genomic DNA. Translation: ACC88495.1.
RefSeqiWP_002211319.1. NZ_CP009780.1.

3D structure databases

ProteinModelPortaliB2KA30.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC88495; ACC88495; YPTS_1523.
KEGGiypb:YPTS_1523.
PATRICi18650864. VBIYerPse20639_1647.

Phylogenomic databases

HOGENOMiHOG000004953.
KOiK00912.
OMAiPTVIYLI.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00482.
BioCyciYPSE502801:GHIH-1642-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00409. LpxK. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR003758. Tetraacyldisaccharide_4-kinase.
[Graphical view]
PfamiPF02606. LpxK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00682. lpxK. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPXK_YERPB
AccessioniPrimary (citable) accession number: B2KA30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: September 7, 2016
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.