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B2K970

- NAPA_YERPB

UniProt

B2K970 - NAPA_YERPB

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Yersinia pseudotuberculosis serotype IB (strain PB1/+)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi81 – 811Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciYPSE502801:GHIH-2996-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:YPTS_2864
OrganismiYersinia pseudotuberculosis serotype IB (strain PB1/+)
Taxonomic identifieri502801 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000001193: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
BLAST
Chaini32 – 830799Periplasmic nitrate reductasePRO_5000345925Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi502801.YPTS_2864.

Structurei

3D structure databases

ProteinModelPortaliB2K970.
SMRiB2K970. Positions 38-827.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 95574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiHPKTRVA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B2K970-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLSRRDFMK ANAAVAAAAA AGMTIPTVAK AVGETTNAIK WDKAPCRFCG
60 70 80 90 100
TGCGVLVGTQ NGRIVASQGD PDSPVNRGLN CIKGYFLPKI MYGKDRLTQP
110 120 130 140 150
LLRMKDGQYD KEGDFTPISW EKAFDIMELK FKNALKEKGP TAVGMFGSGQ
160 170 180 190 200
WTVWEGYAAL KLLKGGFRSN NLDPNARHCM ASSVVGFMRT FGMDEPMGCY
210 220 230 240 250
DDIEEADAFV LWGSNMAEMH PVLWSRMTSR RLTNAHVRIA VLSTYEHRSF
260 270 280 290 300
ELADNPIVFT PQTDLVIMNY IANYIIQNNA VDKDFLAQHV NFRRGATDIG
310 320 330 340 350
YGLRPTHPLE KAAKNPGSDA SEPMSFEDFK TFVAEYTLEK TAKMSGVPED
360 370 380 390 400
QLESLAQLYA DPKVKLVSYW TMGFNQHTRG VWANNMCYNL HLLTGKISTP
410 420 430 440 450
GSGPFSLTGQ PSACGTAREV GTFSHRLPAD MVVTNEKHRQ IAETTWQLPA
460 470 480 490 500
GTIPEKVGLH AVAQDRALKD GTLNAYWVMC NNNMQAGPNI NEERMPGWRD
510 520 530 540 550
PRNFIVVSDP YPTISALSAD LILPTSMWVE KEGAYGNAER RTQFWRQQVP
560 570 580 590 600
SPGEAKSDLW QIVEFAKRFN VEEVWPAELV NQKPEYRGKN LYEVLFANDV
610 620 630 640 650
VSKYPLSEIP DDQLNDEARD FGFYIQKGLF EEYASFGRGH AHDLAPFDVY
660 670 680 690 700
HQVRGLRWPV VDGKETLWRY REGFDPFVPK GEEVRFYGKP DGKAVIFALP
710 720 730 740 750
YEPAAESPDQ EYDLWLSTGR VLEHWHTGSM TRRVPELHRA FPEAVLFIHP
760 770 780 790 800
LDAKARGLHR GDKVKVISRR GEVISLVETR GRNRPPRGLV YMPFFDAAQL
810 820 830
VNNLTLDATD PLSKETDFKK CAVKLERVVA
Length:830
Mass (Da):93,256
Last modified:June 10, 2008 - v1
Checksum:iD2937674E261FE7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001048 Genomic DNA. Translation: ACC89821.1.
RefSeqiYP_001873278.1. NC_010634.1.

Genome annotation databases

EnsemblBacteriaiACC89821; ACC89821; YPTS_2864.
GeneIDi6258032.
KEGGiypb:YPTS_2864.
PATRICi18653847. VBIYerPse20639_3121.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001048 Genomic DNA. Translation: ACC89821.1 .
RefSeqi YP_001873278.1. NC_010634.1.

3D structure databases

ProteinModelPortali B2K970.
SMRi B2K970. Positions 38-827.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 502801.YPTS_2864.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACC89821 ; ACC89821 ; YPTS_2864 .
GeneIDi 6258032.
KEGGi ypb:YPTS_2864.
PATRICi 18653847. VBIYerPse20639_3121.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi HPKTRVA.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci YPSE502801:GHIH-2996-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Yersinia pseudotuberculosis PB1/+."
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C., Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L.
    , Challacombe J.F., Green L., Lindler L.E., Nikolich M.P., Richardson P.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PB1/+.

Entry informationi

Entry nameiNAPA_YERPB
AccessioniPrimary (citable) accession number: B2K970
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 10, 2008
Last modified: November 26, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3