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Protein

Aspartate--ammonia ligase

Gene

asnA

Organism
Yersinia pseudotuberculosis serotype IB (strain PB1/+)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine.UniRule annotation

Pathway:iL-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Aspartate--ammonia ligase (asnA)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (ammonia route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYPSE502801:GHIH-4311-MONOMER.
UniPathwayiUPA00134; UER00194.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--ammonia ligaseUniRule annotation (EC:6.3.1.1UniRule annotation)
Alternative name(s):
Asparagine synthetase AUniRule annotation
Gene namesi
Name:asnAUniRule annotation
Ordered Locus Names:YPTS_4168
OrganismiYersinia pseudotuberculosis serotype IB (strain PB1/+)
Taxonomic identifieri502801 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Aspartate--ammonia ligasePRO_1000129138Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB2K7I9.
SMRiB2K7I9. Positions 5-330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2502.
HOGENOMiHOG000284502.
KOiK01914.
OMAiQSRICMF.
OrthoDBiEOG64V2FB.

Family and domain databases

HAMAPiMF_00555. AsnA.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004618. AsnA.
[Graphical view]
PfamiPF03590. AsnA. 1 hit.
[Graphical view]
PIRSFiPIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMsiTIGR00669. asnA. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2K7I9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKQFIQKQQ QISFVKSFFS RQLEQQLGLI EVQAPILSRV GDGTQDNLSG
60 70 80 90 100
SEKAVQVKVK SLPDSTFEVV HSLAKWKRKT LGRFDFGADQ GVYTHMKALR
110 120 130 140 150
PDEDRLSAIH SVYVDQWDWE RVMGDGERNL AYLKSTVNKI YAAIKETEAA
160 170 180 190 200
ISAEFGVKPF LPDHIQFIHS ESLRARFPDL DAKGRERAIA KELGAVFLIG
210 220 230 240 250
IGGKLADGQS HDVRAPDYDD WTSPSAEGFS GLNGDIIVWN PILEDAFEIS
260 270 280 290 300
SMGIRVDAEA LKRQLALTGD EDRLELEWHQ SLLRGEMPQT IGGGIGQSRL
310 320 330
VMLLLQKQHI GQVQCGVWGP EISEKVDGLL
Length:330
Mass (Da):36,828
Last modified:June 10, 2008 - v1
Checksum:i7B11F8E203509300
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001048 Genomic DNA. Translation: ACC91111.1.
RefSeqiWP_002212256.1. NZ_CP009780.1.

Genome annotation databases

EnsemblBacteriaiACC91111; ACC91111; YPTS_4168.
KEGGiypb:YPTS_4168.
PATRICi18656664. VBIYerPse20639_4508.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001048 Genomic DNA. Translation: ACC91111.1.
RefSeqiWP_002212256.1. NZ_CP009780.1.

3D structure databases

ProteinModelPortaliB2K7I9.
SMRiB2K7I9. Positions 5-330.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC91111; ACC91111; YPTS_4168.
KEGGiypb:YPTS_4168.
PATRICi18656664. VBIYerPse20639_4508.

Phylogenomic databases

eggNOGiCOG2502.
HOGENOMiHOG000284502.
KOiK01914.
OMAiQSRICMF.
OrthoDBiEOG64V2FB.

Enzyme and pathway databases

UniPathwayiUPA00134; UER00194.
BioCyciYPSE502801:GHIH-4311-MONOMER.

Family and domain databases

HAMAPiMF_00555. AsnA.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004618. AsnA.
[Graphical view]
PfamiPF03590. AsnA. 1 hit.
[Graphical view]
PIRSFiPIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMsiTIGR00669. asnA. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Yersinia pseudotuberculosis PB1/+."
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C., Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L.
    , Challacombe J.F., Green L., Lindler L.E., Nikolich M.P., Richardson P.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PB1/+.

Entry informationi

Entry nameiASNA_YERPB
AccessioniPrimary (citable) accession number: B2K7I9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: July 22, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.