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B2K6E6

- BGAL_YERPB

UniProt

B2K6E6 - BGAL_YERPB

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Protein

Beta-galactosidase

Gene

lacZ

Organism
Yersinia pseudotuberculosis serotype IB (strain PB1/+)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer.UniRule annotation
Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101SubstrateUniRule annotation
Metal bindingi209 – 2091SodiumUniRule annotation
Binding sitei209 – 2091SubstrateUniRule annotation
Sitei373 – 3731Transition state stabilizerUniRule annotation
Sitei407 – 4071Transition state stabilizerUniRule annotation
Metal bindingi432 – 4321Magnesium 1UniRule annotation
Metal bindingi434 – 4341Magnesium 1UniRule annotation
Active sitei477 – 4771Proton donorUniRule annotation
Metal bindingi477 – 4771Magnesium 1UniRule annotation
Binding sitei477 – 4771SubstrateUniRule annotation
Active sitei553 – 5531NucleophileUniRule annotation
Metal bindingi613 – 6131Magnesium 2UniRule annotation
Metal bindingi617 – 6171Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi620 – 6201SodiumUniRule annotation
Binding sitei620 – 6201SubstrateUniRule annotation
Binding sitei1041 – 10411SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciYPSE502801:GHIH-2621-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:YPTS_2494
OrganismiYersinia pseudotuberculosis serotype IB (strain PB1/+)
Taxonomic identifieri502801 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000001193: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10661066Beta-galactosidasePRO_0000367021Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi502801.YPTS_2494.

Structurei

3D structure databases

ProteinModelPortaliB2K6E6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni553 – 5564Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiNPPFVPK.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2K6E6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSQEKVPFQ VQLSLPQILS RRDWENPQIT QYHRLEAHPP FHSWRDVESA
60 70 80 90 100
QKDRPSPQQQ TLNGLWSFSY FTQPEAVPEH WVRCDLAEAK PLPVPANWQL
110 120 130 140 150
HGYDAPIYTN IQYPIPVNPP RVPDLNPTGC YSRDFTLEPS WLASGKTRII
160 170 180 190 200
FDGVSSAFYL WCNGQWVGYS QDSRLPAEFD LTPYLQAGSN RIAVLVLRWS
210 220 230 240 250
DGSYLEDQDM WRMSGIFRDV KLLHKPEIHL RDIHIMTHLS PEFTSANLEV
260 270 280 290 300
MAAVNIPSLQ LNDPQVTGSY QLRVQLWLAD KLVASLQQPL GTQAIDERGP
310 320 330 340 350
YTDRTQLVLR IDQPLLWSAE QPTLYRAVVS LLNHQQELIE AEAYDVGFRQ
360 370 380 390 400
VAIHQGLLKI NGKAVLIRGV NRHEHHPQTG QAIDEESLLQ DILLMKQHNF
410 420 430 440 450
NAVRCSHYPN HPLWYRLCDR YGLYVVDEAN IETHGMQPMS RLSDDPSWFS
460 470 480 490 500
AFSERVTRMV QRDRNHPCII IWSLGNESGH GATHDALYRW IKTNDPTRPV
510 520 530 540 550
QYEGGGANTL ATDILCPMYA RVDEDQPFPA VPKWSIKKWI GLPNESRPLI
560 570 580 590 600
LCEYAHAMGN SFGGFARYWQ AFRQYPRLQG GFIWDWVDQS LTHHNDHGQP
610 620 630 640 650
YWAYGGDFGD TPNDRQFCMN GLVFPDRSPH PSLYEAQCAQ QFFQFSLLST
660 670 680 690 700
TPLVINITSE YLFRESDNEQ LYWRIMLEGE SVLEGSQPLN LSPESSQCYR
710 720 730 740 750
LAEKLPTLNK PGQLWLNVEI RQPKETPWSP AQHRSAWHQW RLPQPLFSPS
760 770 780 790 800
SDLTNATAHY APQLQHNLQL QHNRQLQHDL QLQQDEQHIK VTYQQQCWQF
810 820 830 840 850
SRQTGRLDQW WVADKPMLLR PLQDQFVRAP LDNDIGISEA THIDPNAWVE
860 870 880 890 900
RWKKAGMYQL QQRCLSLHVD HLSHSVQISA EYGYEFEQEP LLHSHWVYRF
910 920 930 940 950
DRHGRMTIDV NVRIATSLPA PARIGMCCQL ADISPTVEWL GLGPHENYPD
960 970 980 990 1000
RQLAAQYGHW SLPLEQMHTA YIFPSENGLR CNTHTLNYGR WTLTGDFHFG
1010 1020 1030 1040 1050
ISRYSTQQLM VTSHQHLLEP EEGTWLNIDG FHMGVGGDDS WSPSVHIDDI
1060
LTRETYQYQI CWQYKV
Length:1,066
Mass (Da):123,480
Last modified:June 10, 2008 - v1
Checksum:i6BF435A8129D49D4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001048 Genomic DNA. Translation: ACC89455.1.
RefSeqiYP_001872912.1. NC_010634.1.

Genome annotation databases

EnsemblBacteriaiACC89455; ACC89455; YPTS_2494.
GeneIDi6257657.
KEGGiypb:YPTS_2494.
PATRICi18653000. VBIYerPse20639_2705.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001048 Genomic DNA. Translation: ACC89455.1 .
RefSeqi YP_001872912.1. NC_010634.1.

3D structure databases

ProteinModelPortali B2K6E6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 502801.YPTS_2494.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACC89455 ; ACC89455 ; YPTS_2494 .
GeneIDi 6257657.
KEGGi ypb:YPTS_2494.
PATRICi 18653000. VBIYerPse20639_2705.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi NPPFVPK.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci YPSE502801:GHIH-2621-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Yersinia pseudotuberculosis PB1/+."
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C., Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L.
    , Challacombe J.F., Green L., Lindler L.E., Nikolich M.P., Richardson P.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PB1/+.

Entry informationi

Entry nameiBGAL_YERPB
AccessioniPrimary (citable) accession number: B2K6E6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: October 1, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3