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B2K6E6

- BGAL_YERPB

UniProt

B2K6E6 - BGAL_YERPB

Protein

Beta-galactosidase

Gene

lacZ

Organism
Yersinia pseudotuberculosis serotype IB (strain PB1/+)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei110 – 1101SubstrateUniRule annotation
    Metal bindingi209 – 2091SodiumUniRule annotation
    Binding sitei209 – 2091SubstrateUniRule annotation
    Sitei373 – 3731Transition state stabilizerUniRule annotation
    Sitei407 – 4071Transition state stabilizerUniRule annotation
    Metal bindingi432 – 4321Magnesium 1UniRule annotation
    Metal bindingi434 – 4341Magnesium 1UniRule annotation
    Active sitei477 – 4771Proton donorUniRule annotation
    Metal bindingi477 – 4771Magnesium 1UniRule annotation
    Binding sitei477 – 4771SubstrateUniRule annotation
    Active sitei553 – 5531NucleophileUniRule annotation
    Metal bindingi613 – 6131Magnesium 2UniRule annotation
    Metal bindingi617 – 6171Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi620 – 6201SodiumUniRule annotation
    Binding sitei620 – 6201SubstrateUniRule annotation
    Binding sitei1041 – 10411SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Enzyme and pathway databases

    BioCyciYPSE502801:GHIH-2621-MONOMER.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:YPTS_2494
    OrganismiYersinia pseudotuberculosis serotype IB (strain PB1/+)
    Taxonomic identifieri502801 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
    ProteomesiUP000001193: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10661066Beta-galactosidasePRO_0000367021Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi502801.YPTS_2494.

    Structurei

    3D structure databases

    ProteinModelPortaliB2K6E6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni553 – 5564Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiNPPFVPK.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2K6E6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSQEKVPFQ VQLSLPQILS RRDWENPQIT QYHRLEAHPP FHSWRDVESA     50
    QKDRPSPQQQ TLNGLWSFSY FTQPEAVPEH WVRCDLAEAK PLPVPANWQL 100
    HGYDAPIYTN IQYPIPVNPP RVPDLNPTGC YSRDFTLEPS WLASGKTRII 150
    FDGVSSAFYL WCNGQWVGYS QDSRLPAEFD LTPYLQAGSN RIAVLVLRWS 200
    DGSYLEDQDM WRMSGIFRDV KLLHKPEIHL RDIHIMTHLS PEFTSANLEV 250
    MAAVNIPSLQ LNDPQVTGSY QLRVQLWLAD KLVASLQQPL GTQAIDERGP 300
    YTDRTQLVLR IDQPLLWSAE QPTLYRAVVS LLNHQQELIE AEAYDVGFRQ 350
    VAIHQGLLKI NGKAVLIRGV NRHEHHPQTG QAIDEESLLQ DILLMKQHNF 400
    NAVRCSHYPN HPLWYRLCDR YGLYVVDEAN IETHGMQPMS RLSDDPSWFS 450
    AFSERVTRMV QRDRNHPCII IWSLGNESGH GATHDALYRW IKTNDPTRPV 500
    QYEGGGANTL ATDILCPMYA RVDEDQPFPA VPKWSIKKWI GLPNESRPLI 550
    LCEYAHAMGN SFGGFARYWQ AFRQYPRLQG GFIWDWVDQS LTHHNDHGQP 600
    YWAYGGDFGD TPNDRQFCMN GLVFPDRSPH PSLYEAQCAQ QFFQFSLLST 650
    TPLVINITSE YLFRESDNEQ LYWRIMLEGE SVLEGSQPLN LSPESSQCYR 700
    LAEKLPTLNK PGQLWLNVEI RQPKETPWSP AQHRSAWHQW RLPQPLFSPS 750
    SDLTNATAHY APQLQHNLQL QHNRQLQHDL QLQQDEQHIK VTYQQQCWQF 800
    SRQTGRLDQW WVADKPMLLR PLQDQFVRAP LDNDIGISEA THIDPNAWVE 850
    RWKKAGMYQL QQRCLSLHVD HLSHSVQISA EYGYEFEQEP LLHSHWVYRF 900
    DRHGRMTIDV NVRIATSLPA PARIGMCCQL ADISPTVEWL GLGPHENYPD 950
    RQLAAQYGHW SLPLEQMHTA YIFPSENGLR CNTHTLNYGR WTLTGDFHFG 1000
    ISRYSTQQLM VTSHQHLLEP EEGTWLNIDG FHMGVGGDDS WSPSVHIDDI 1050
    LTRETYQYQI CWQYKV 1066
    Length:1,066
    Mass (Da):123,480
    Last modified:June 10, 2008 - v1
    Checksum:i6BF435A8129D49D4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001048 Genomic DNA. Translation: ACC89455.1.
    RefSeqiYP_001872912.1. NC_010634.1.

    Genome annotation databases

    EnsemblBacteriaiACC89455; ACC89455; YPTS_2494.
    GeneIDi6257657.
    KEGGiypb:YPTS_2494.
    PATRICi18653000. VBIYerPse20639_2705.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001048 Genomic DNA. Translation: ACC89455.1 .
    RefSeqi YP_001872912.1. NC_010634.1.

    3D structure databases

    ProteinModelPortali B2K6E6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 502801.YPTS_2494.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACC89455 ; ACC89455 ; YPTS_2494 .
    GeneIDi 6257657.
    KEGGi ypb:YPTS_2494.
    PATRICi 18653000. VBIYerPse20639_2705.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi NPPFVPK.
    OrthoDBi EOG6XWV0T.

    Enzyme and pathway databases

    BioCyci YPSE502801:GHIH-2621-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Yersinia pseudotuberculosis PB1/+."
      Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C., Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L.
      , Challacombe J.F., Green L., Lindler L.E., Nikolich M.P., Richardson P.
      Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PB1/+.

    Entry informationi

    Entry nameiBGAL_YERPB
    AccessioniPrimary (citable) accession number: B2K6E6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3