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B2JX50

- RBL_BURP8

UniProt

B2JX50 - RBL_BURP8

Protein

Ribulose bisphosphate carboxylase large chain

Gene

cbbL

Organism
Burkholderia phymatum (strain DSM 17167 / STM815)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.UniRule annotation

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei141 – 1411Substrate; in homodimeric partnerUniRule annotation
    Binding sitei191 – 1911SubstrateUniRule annotation
    Active sitei193 – 1931Proton acceptorUniRule annotation
    Binding sitei195 – 1951SubstrateUniRule annotation
    Metal bindingi219 – 2191Magnesium; via carbamate groupUniRule annotation
    Metal bindingi221 – 2211MagnesiumUniRule annotation
    Metal bindingi222 – 2221MagnesiumUniRule annotation
    Active sitei311 – 3111Proton acceptorUniRule annotation
    Binding sitei312 – 3121SubstrateUniRule annotation
    Binding sitei344 – 3441SubstrateUniRule annotation
    Sitei351 – 3511Transition state stabilizerUniRule annotation
    Binding sitei396 – 3961SubstrateUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. monooxygenase activity Source: UniProtKB-KW
    3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. reductive pentose-phosphate cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase, Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Calvin cycle, Carbon dioxide fixation

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBPHY391038:GI4Z-6575-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylase large chainUniRule annotation (EC:4.1.1.39UniRule annotation)
    Short name:
    RuBisCO large subunitUniRule annotation
    Gene namesi
    Name:cbbLUniRule annotation
    Ordered Locus Names:Bphy_6497
    Encoded oniPlasmid pBPHY010 Publication
    OrganismiBurkholderia phymatum (strain DSM 17167 / STM815)
    Taxonomic identifieri391038 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
    ProteomesiUP000001192: Plasmid pBPHY01

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 501501Ribulose bisphosphate carboxylase large chainPRO_0000355748Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei219 – 2191N6-carboxylysineUniRule annotation

    Proteomic databases

    PRIDEiB2JX50.

    Interactioni

    Subunit structurei

    Heterohexadecamer of 8 large chains and 8 small chains.UniRule annotation

    Protein-protein interaction databases

    STRINGi391038.Bphy_6497.

    Structurei

    3D structure databases

    ProteinModelPortaliB2JX50.
    SMRiB2JX50. Positions 22-495.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuBisCO large chain family. Type I subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1850.
    HOGENOMiHOG000230831.
    KOiK01601.
    OMAiCTPLKQA.
    OrthoDBiEOG6ZKXMS.

    Family and domain databases

    Gene3Di3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPiMF_01338. RuBisCO_L_type1.
    InterProiIPR020878. RuBisCo_large_chain_AS.
    IPR020888. RuBisCO_lsu.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view]
    PfamiPF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2JX50-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNDFSKEAVK PADSATAAAK AEKRSRYAAG VMKYREMGYW QPDYTPKDTD    50
    VIALFRITPQ PGVEPEEAAA AVAGESSTAT WTVVWTDRLT ACDMYRAKAF 100
    RVEPVPNPAE GEPQYFAFIA YELDLFEEGS VANLTASIIG NVFGFKPLKA 150
    LRLEDMRIPV AYLKTFQGPP TGIVVERERL DKYGRPLLGA TVKPKLGLSG 200
    KNYGRVVYEG LKGGLDFLKD DENINSQPFM HWRDRYLFAM EAVHRAQAET 250
    GEVKGHYLNV TAGTMEDMYE RAEFAKELGS CIVMIDLVIG WTAITSMGRW 300
    ARKNDMILHL HRAGHGTYTR QRNHGISFRV IAKWLRMAGV DHAHAGTAVG 350
    KLDGDPLSVQ GYYNVLRESH NSVDLTRGIF FDQHWAGLRK VMPVASGGIH 400
    AGQMHQLLDL FGDDAILQFG GGTIGHPSGI QAGATANRVA LETMVKARNE 450
    GRDIANEGSD LLEAAARHCT PLKQALDTWG DVTFNYTPTD SPDFAVTPSV 500
    A 501
    Length:501
    Mass (Da):55,176
    Last modified:June 10, 2008 - v1
    Checksum:iE8238E2EFE685A2D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001045 Genomic DNA. Translation: ACC75527.1.
    RefSeqiYP_001862573.1. NC_010625.1.

    Genome annotation databases

    EnsemblBacteriaiACC75527; ACC75527; Bphy_6497.
    GeneIDi6248036.
    KEGGibph:Bphy_6497.
    PATRICi19199896. VBIBurPhy25146_6827.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001045 Genomic DNA. Translation: ACC75527.1 .
    RefSeqi YP_001862573.1. NC_010625.1.

    3D structure databases

    ProteinModelPortali B2JX50.
    SMRi B2JX50. Positions 22-495.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 391038.Bphy_6497.

    Proteomic databases

    PRIDEi B2JX50.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACC75527 ; ACC75527 ; Bphy_6497 .
    GeneIDi 6248036.
    KEGGi bph:Bphy_6497.
    PATRICi 19199896. VBIBurPhy25146_6827.

    Phylogenomic databases

    eggNOGi COG1850.
    HOGENOMi HOG000230831.
    KOi K01601.
    OMAi CTPLKQA.
    OrthoDBi EOG6ZKXMS.

    Enzyme and pathway databases

    BioCyci BPHY391038:GI4Z-6575-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPi MF_01338. RuBisCO_L_type1.
    InterProi IPR020878. RuBisCo_large_chain_AS.
    IPR020888. RuBisCO_lsu.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view ]
    Pfami PF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    PROSITEi PS00157. RUBISCO_LARGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 17167 / STM815.

    Entry informationi

    Entry nameiRBL_BURP8
    AccessioniPrimary (citable) accession number: B2JX50
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 16, 2008
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel".UniRule annotation

    Keywords - Technical termi

    Complete proteome, Plasmid, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3