Reviewed, UniProtKB/Swiss-Prot B2JS89 (BETA_BURP8)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	Bphy_3310

Organism

Burkholderia phymatum (strain DSM 17167 / STM815) [Complete proteome] [HAMAP]

Taxonomic identifier

391038 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Choline dehydrogenase HAMAP MF_00750		PRO_1000133324
Regions
	Nucleotide binding	7 – 36	30	FAD Probable
Sites
	Active site	474	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2JS89-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 8484418C99549861	FASTA	572	63,110
	10 20 30 40 50 60 MAAKEYDYII IGAGSAGNVL ATRLTEDRDV TVLLLEAGGP DYRFDFRTQM PAALAYPLQG 70 80 90 100 110 120 RRYNWAYETD PEPFMNNRRM ECGRGKGLGG SSLINGMCYI RGNALDYDGW AERKGLENWT 130 140 150 160 170 180 YLDCLPYFRK AETRDAGAND YHGGDGPVHV TTSKRGVNPL FEAMVEAGVQ AGYPRTDDLN 190 200 210 220 230 240 GYQQEGFGPM DRTVTANGRR ASTARGYLDQ ARPRPNLTIV TYATTDRILF SGKRAQGVVY 250 260 270 280 290 300 LDGQAQITAH ARREVLLCSG AIASPQILQR SGVGPGGWLR DLDIPVVLDL PGVGQNLQDH 310 320 330 340 350 360 LEMYMQYECK EPVSLYPALL LRNQPAIGIE WMLKGTGIGA SNHFEAGGFI RTRDDDPWPN 370 380 390 400 410 420 IQYHFLPVAI NYNGTNAIKM HGFQAHVGSM RSPSRGRVKL RSRDPREHPS ILFNYMAEAL 430 440 450 460 470 480 DWREFRDAIR ITREIIAQPA LDRFRGRELS PGAELQSDAQ IDAFVRARAE TAYHPSCSCA 490 500 510 520 530 540 MGYDDMAVVD GEGRVHGLEG LRVVDASIMP RITTGNLNAP TIMLAEKIAD RIRGRAPLAR 550 560 570 STAPYYVANG APARGAKHVE HAPAPSVAAH TH

References

[1]

"Complete sequence of chromosome 2 of Burkholderia phymatum STM815." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Bruce D., Goodwin L., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Bacher J., Blanchard J., Cohan F., James E., Lawrence J., Lizotte-Waniewski M., Moulin L., Rainey P., Riley M., Souza V., Wertz J., Young P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001044 Genomic DNA. Translation: ACC72466.1.
RefSeq	YP_001859512.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6244741.
GenomeReviews	Gene locus Bphy_3310 in contig CP001044_GR.
KEGG	bph:Bphy_3310.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	PNLQYHF.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_BURP8

Accession

Primary (citable) accession number: B2JS89

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)