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Protein

NAD/NADP-dependent betaine aldehyde dehydrogenase

Gene

betB

Organism
Burkholderia phymatum (strain DSM 17167 / STM815)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the reversible oxidation of betaine aldehyde to the corresponding acid.UniRule annotation

Catalytic activityi

Betaine aldehyde + NAD+ + H2O = betaine + NADH.UniRule annotation

Cofactori

K(+)UniRule annotationNote: Binds 2 potassium ions per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi26 – 261Potassium 1UniRule annotation
Metal bindingi93 – 931Potassium 1UniRule annotation
Active sitei162 – 1621Charge relay systemUniRule annotation
Metal bindingi180 – 1801Potassium 1; via carbonyl oxygenUniRule annotation
Binding sitei209 – 2091NAD/NADP; via amide nitrogenUniRule annotation
Metal bindingi245 – 2451Potassium 2; via carbonyl oxygenUniRule annotation
Sitei247 – 2471Seems to be a necessary countercharge to the potassium cationsUniRule annotation
Active sitei251 – 2511Proton acceptorUniRule annotation
Binding sitei285 – 2851NAD/NADPUniRule annotation
Binding sitei386 – 3861NAD/NADPUniRule annotation
Metal bindingi456 – 4561Potassium 2; via carbonyl oxygenUniRule annotation
Metal bindingi459 – 4591Potassium 2; via carbonyl oxygenUniRule annotation
Active sitei463 – 4631Charge relay systemUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi150 – 1534NAD/NADPUniRule annotation
Nucleotide bindingi176 – 1794NAD/NADPUniRule annotation
Nucleotide bindingi228 – 2336NAD/NADPUniRule annotation

GO - Molecular functioni

  1. betaine-aldehyde dehydrogenase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glycine betaine biosynthetic process from choline Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, NADP, Potassium

Enzyme and pathway databases

BioCyciBPHY391038:GI4Z-3378-MONOMER.
UniPathwayiUPA00529; UER00386.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD/NADP-dependent betaine aldehyde dehydrogenaseUniRule annotation (EC:1.2.1.8UniRule annotation)
Short name:
BADHUniRule annotation
Gene namesi
Name:betBUniRule annotation
Ordered Locus Names:Bphy_3309
OrganismiBurkholderia phymatum (strain DSM 17167 / STM815)
Taxonomic identifieri391038 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
ProteomesiUP000001192 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489NAD/NADP-dependent betaine aldehyde dehydrogenasePRO_1000133944Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei285 – 2851Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi391038.Bphy_3309.

Structurei

3D structure databases

ProteinModelPortaliB2JS88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271505.
KOiK00130.
OMAiKDGYYMR.
OrthoDBiEOG6BS8QW.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_00804. BADH.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01804. BADH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2JS88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAYGLQRLY IGGDYVDATS GVTFDTFDPA TGELLATVQQ ASEADIERAV
60 70 80 90 100
QSAREGQRAW AAMTAMQRSR ILRRAVDLLR ERNDELAELE MRDTGKPIAE
110 120 130 140 150
TRAVDIVTGA DVIEYYAGLA TAIEGQQIPL RAESFVYTRR EPLGVTAGIG
160 170 180 190 200
AWNYPIQIAC WKSAPALAAG NAMIFKPSEV TPLSASKLAE IYLEAGVPAG
210 220 230 240 250
VFNVVQGDGR VGAMLSAHPG IAKISFTGGV ETGKKVMSMA GGSSLKEVTM
260 270 280 290 300
ELGGKSPLVV FEDADLDRAA DIAVTANFFS AGQVCTNGTR VFVHQSVQPA
310 320 330 340 350
FEARVIERVK RIRVGKPSDP STNFGPLASA AQLDKVLGFI ESGKREGARL
360 370 380 390 400
VAGGARIVEG DYARGQYVQP TVFSDCRDDM KIVREEIFGP VMSILSFVDE
410 420 430 440 450
DDVIERANDT IYGLAAGVVT ENLARAHRAI HRLEAGICWI NTWGESPAEM
460 470 480
PVGGYKQSGV GRENGITTLE HYTRIKSVQV ELGKYQPVF
Length:489
Mass (Da):52,647
Last modified:June 10, 2008 - v1
Checksum:iD0EC8BB1F4E493A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001044 Genomic DNA. Translation: ACC72465.1.
RefSeqiWP_012402638.1. NC_010623.1.
YP_001859511.1. NC_010623.1.

Genome annotation databases

EnsemblBacteriaiACC72465; ACC72465; Bphy_3309.
KEGGibph:Bphy_3309.
PATRICi19193203. VBIBurPhy25146_3494.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001044 Genomic DNA. Translation: ACC72465.1.
RefSeqiWP_012402638.1. NC_010623.1.
YP_001859511.1. NC_010623.1.

3D structure databases

ProteinModelPortaliB2JS88.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391038.Bphy_3309.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC72465; ACC72465; Bphy_3309.
KEGGibph:Bphy_3309.
PATRICi19193203. VBIBurPhy25146_3494.

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271505.
KOiK00130.
OMAiKDGYYMR.
OrthoDBiEOG6BS8QW.

Enzyme and pathway databases

UniPathwayiUPA00529; UER00386.
BioCyciBPHY391038:GI4Z-3378-MONOMER.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_00804. BADH.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01804. BADH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17167 / STM815.

Entry informationi

Entry nameiBETB_BURP8
AccessioniPrimary (citable) accession number: B2JS88
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: April 29, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.