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Protein

4-hydroxy-2-oxovalerate aldolase 2

Gene

Bphy_4539

Organism
Burkholderia phymatum (strain DSM 17167 / STM815)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the retro-aldol cleavage of 4-hydroxy-2-oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds.UniRule annotation

Catalytic activityi

(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei13 – 131Transition state stabilizerUniRule annotation
Metal bindingi14 – 141ManganeseUniRule annotation
Active sitei17 – 171Proton acceptorUniRule annotation
Binding sitei167 – 1671SubstrateUniRule annotation
Metal bindingi196 – 1961Manganese; via tele nitrogenUniRule annotation
Binding sitei196 – 1961SubstrateUniRule annotation
Metal bindingi198 – 1981Manganese; via tele nitrogenUniRule annotation
Binding sitei287 – 2871SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciBPHY391038:GI4Z-4613-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-2-oxovalerate aldolase 2UniRule annotation (EC:4.1.3.39UniRule annotation)
Short name:
HOA 2UniRule annotation
Alternative name(s):
4-hydroxy-2-keto-pentanoic acid aldolase 2UniRule annotation
4-hydroxy-2-oxopentanoate aldolase 2UniRule annotation
Gene namesi
Ordered Locus Names:Bphy_4539
OrganismiBurkholderia phymatum (strain DSM 17167 / STM815)
Taxonomic identifieri391038 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
ProteomesiUP000001192 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3393394-hydroxy-2-oxovalerate aldolase 2PRO_0000387797Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi391038.Bphy_4539.

Structurei

3D structure databases

ProteinModelPortaliB2JQW0.
SMRiB2JQW0. Positions 3-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni13 – 142Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the 4-hydroxy-2-oxovalerate aldolase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000048047.
KOiK01666.
OMAiDIAWIEA.
OrthoDBiEOG6C2WC0.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01656. HOA.
InterProiIPR017629. 4OH_2_O-val_aldolase.
IPR013785. Aldolase_TIM.
IPR012425. DmpG_comm.
IPR000891. PYR_CT.
[Graphical view]
PANTHERiPTHR10277:SF3. PTHR10277:SF3. 1 hit.
PfamiPF07836. DmpG_comm. 1 hit.
PF00682. HMGL-like. 1 hit.
[Graphical view]
ProDomiPD005364. DmpG_comm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITEiPS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2JQW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKKLYISDV TLRDGMHAIR HQYSIQNVQD IARALDEAKV DSIEVAHGDG
60 70 80 90 100
LQGSSFNYGF GAHSDLEWIE AVADVVRHAK IATLLLPGIG TIHDLKAAYD
110 120 130 140 150
AGARVVRVAT HCTEADISKQ HIEYARSLGM DTVGFLMMSH MTTPENLAVE
160 170 180 190 200
AKKMESYGAT CVYVVDSGGA MNMNDIRARF MALKATLDPA TQTGMHAHHN
210 220 230 240 250
LSLGVANSIV AVEEGCDRVD ASLAGMGAGA GNAPLEVFIA AVERMGWHHG
260 270 280 290 300
TDLYRLLDAA DDIVRPLQDR PVRVDRETLA LGYAGVYSSF LRHSEIAANK
310 320 330
YGLKAVDILV ELGKRRMVGG QEDMIVDVAL DLKKREAHA
Length:339
Mass (Da):36,794
Last modified:June 10, 2008 - v1
Checksum:i3897287A46C86A9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001044 Genomic DNA. Translation: ACC73651.1.
RefSeqiWP_012403823.1. NC_010623.1.

Genome annotation databases

EnsemblBacteriaiACC73651; ACC73651; Bphy_4539.
KEGGibph:Bphy_4539.
PATRICi19195821. VBIBurPhy25146_4797.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001044 Genomic DNA. Translation: ACC73651.1.
RefSeqiWP_012403823.1. NC_010623.1.

3D structure databases

ProteinModelPortaliB2JQW0.
SMRiB2JQW0. Positions 3-332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391038.Bphy_4539.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC73651; ACC73651; Bphy_4539.
KEGGibph:Bphy_4539.
PATRICi19195821. VBIBurPhy25146_4797.

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000048047.
KOiK01666.
OMAiDIAWIEA.
OrthoDBiEOG6C2WC0.

Enzyme and pathway databases

BioCyciBPHY391038:GI4Z-4613-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01656. HOA.
InterProiIPR017629. 4OH_2_O-val_aldolase.
IPR013785. Aldolase_TIM.
IPR012425. DmpG_comm.
IPR000891. PYR_CT.
[Graphical view]
PANTHERiPTHR10277:SF3. PTHR10277:SF3. 1 hit.
PfamiPF07836. DmpG_comm. 1 hit.
PF00682. HMGL-like. 1 hit.
[Graphical view]
ProDomiPD005364. DmpG_comm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITEiPS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17167 / STM815.

Entry informationi

Entry nameiHOA2_BURP8
AccessioniPrimary (citable) accession number: B2JQW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: June 10, 2008
Last modified: July 22, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.