Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B2JQW0 (HOA2_BURP8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxy-2-oxovalerate aldolase 2

Short name=HOA 2
EC=4.1.3.39
Alternative name(s):
4-hydroxy-2-keto-pentanoic acid aldolase 2
4-hydroxy-2-oxopentanoate aldolase 2
Gene names
Ordered Locus Names:Bphy_4539
OrganismBurkholderia phymatum (strain DSM 17167 / STM815) [Complete proteome] [HAMAP]
Taxonomic identifier391038 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia

Protein attributes

Sequence length339 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the retro-aldol cleavage of 4-hydroxy-2-oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds By similarity. HAMAP-Rule MF_01656

Catalytic activity

(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate. HAMAP-Rule MF_01656

Sequence similarities

Belongs to the 4-hydroxy-2-oxovalerate aldolase family.

Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism
   LigandManganese
Metal-binding
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processaromatic compound catabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_function4-hydroxy-2-oxovalerate aldolase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3393394-hydroxy-2-oxovalerate aldolase 2 HAMAP-Rule MF_01656
PRO_0000387797

Regions

Region13 – 142Substrate binding By similarity

Sites

Active site171Proton acceptor Potential
Metal binding141Manganese By similarity
Metal binding1961Manganese; via tele nitrogen By similarity
Metal binding1981Manganese; via tele nitrogen By similarity
Binding site1671Substrate By similarity
Binding site1961Substrate By similarity
Binding site2871Substrate By similarity
Site131Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
B2JQW0 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 3897287A46C86A9C

FASTA33936,794
        10         20         30         40         50         60 
MDKKLYISDV TLRDGMHAIR HQYSIQNVQD IARALDEAKV DSIEVAHGDG LQGSSFNYGF 

        70         80         90        100        110        120 
GAHSDLEWIE AVADVVRHAK IATLLLPGIG TIHDLKAAYD AGARVVRVAT HCTEADISKQ 

       130        140        150        160        170        180 
HIEYARSLGM DTVGFLMMSH MTTPENLAVE AKKMESYGAT CVYVVDSGGA MNMNDIRARF 

       190        200        210        220        230        240 
MALKATLDPA TQTGMHAHHN LSLGVANSIV AVEEGCDRVD ASLAGMGAGA GNAPLEVFIA 

       250        260        270        280        290        300 
AVERMGWHHG TDLYRLLDAA DDIVRPLQDR PVRVDRETLA LGYAGVYSSF LRHSEIAANK 

       310        320        330 
YGLKAVDILV ELGKRRMVGG QEDMIVDVAL DLKKREAHA 

« Hide

References

[1]"Complete sequence of chromosome 2 of Burkholderia phymatum STM815."
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Bruce D., Goodwin L., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Bacher J., Blanchard J., Cohan F., James E., Lawrence J., Lizotte-Waniewski M., Moulin L., Rainey P., Riley M., Souza V., Wertz J., Young P.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17167 / STM815.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001044 Genomic DNA. Translation: ACC73651.1.
RefSeqYP_001860697.1. NC_010623.1.

3D structure databases

ProteinModelPortalB2JQW0.
SMRB2JQW0. Positions 3-332.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391038.Bphy_4539.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACC73651; ACC73651; Bphy_4539.
GeneID6246058.
KEGGbph:Bphy_4539.
PATRIC19195821. VBIBurPhy25146_4797.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0119.
HOGENOMHOG000048047.
KOK01666.
OMAVGTHCTE.
OrthoDBEOG6C2WC0.

Enzyme and pathway databases

BioCycBPHY391038:GI4Z-4613-MONOMER.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01656. HOA.
InterProIPR017629. 4OH_2_O-val_aldolase.
IPR013785. Aldolase_TIM.
IPR012425. DmpG_comm.
IPR000891. PYR_CT.
[Graphical view]
PANTHERPTHR10277:SF3. PTHR10277:SF3. 1 hit.
PfamPF07836. DmpG_comm. 1 hit.
PF00682. HMGL-like. 1 hit.
[Graphical view]
ProDomPD005364. DmpG_comm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITEPS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHOA2_BURP8
AccessionPrimary (citable) accession number: B2JQW0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: June 10, 2008
Last modified: May 14, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families