B2JNZ4 (METE_BURP8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	Bphy_5470

Organism

Burkholderia phymatum (strain DSM 17167 / STM815) [Complete proteome] [HAMAP]

Taxonomic identifier

391038 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	763 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 763	763	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000097820
Sites
	Metal binding	650	1	Zinc By similarity
	Metal binding	652	1	Zinc By similarity
	Metal binding	735	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2JNZ4 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 9B468EECD01C8A5E	FASTA	763	84,900
	10 20 30 40 50 60 MVTTHNLGFP RIGAHRELKF ALEKYWKGES SRAELKAVGA QLRARHWQDQ ARLDFSPVGD 70 80 90 100 110 120 FAFYDQVLDM SFTLGNLPER VRGFHGDALD NAFRVARGRS AHGADDHSAC CGGVAAGEMT 130 140 150 160 170 180 KWFDTNYHYI VPEFDTNTQF SLDPSRLLEQ IKEAHAQGVK AKPVIIGPVT YLWLGKAKDG 190 200 210 220 230 240 SDKLTLLPRL LPVYAALLDY FTAQGIDWVQ IDEPVLVTEL DARWRDAFVP AYDALAARRV 250 260 270 280 290 300 RVLLATYFGQ LKENLELACQ LPVDGLHIDA IHARDEVAQV AAQVPETAVL SVGVINGRNV 310 320 330 340 350 360 WKTDLNAALA WLEPLHATLG DRLWIAPSCS LLHSPVDLNS ERKLDADIRS WLAFALQKLD 370 380 390 400 410 420 ELTLLASALN NGRACVEAEL LANAKAIESR RASPRVHNAA VKAALARIDA SLGQRANAYP 430 440 450 460 470 480 ARAAKQAAAL ALPAFPTTTI GSFPQTADIR RARSQFKAGE LDYAGYKLAM EREITRAVKE 490 500 510 520 530 540 QETLGLDVLV HGEAERNDMV EYFGEQLDGY VFSQFGWVQS YGSRCVKPPI LFGDISRPKA 550 560 570 580 590 600 MTVEWICYAQ AQTAKPMKGM LTGPVTILNW SFVRDDQPRS VSCRQLALAI REEVLDLEKA 610 620 630 640 650 660 GVRVIQIDEA ALREGLPLRR SQWNEYLQWA VESFRIAANG VQDETQIHTH MCYSEFNDII 670 680 690 700 710 720 ASIAEMDADV ITIETSRSDM ELLDAFDDFH YPNQIGPGVY DIHSPNIPDQ AHVVDLMKKA 730 740 750 760 AERIPAERLW VNPDCGLKTR AWEEVIPALK NMVAAARTLR QAV

References

[1]

"Complete sequence of chromosome 2 of Burkholderia phymatum STM815." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Bruce D., Goodwin L., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Bacher J., Blanchard J., Cohan F., James E., Lawrence J., Lizotte-Waniewski M., Moulin L., Rainey P., Riley M., Souza V., Wertz J., Young P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17167 / STM815.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001044 Genomic DNA. Translation: ACC74547.1.
RefSeq	YP_001861593.1. NC_010623.1.
3D structure databases
ProteinModelPortal	B2JNZ4.
SMR	B2JNZ4. Positions 3-761.
ModBase	Search...
Protein-protein interaction databases
STRING	B2JNZ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6246954.
GenomeReviews	Gene locus Bphy_5470 in contig CP001044_GR.
KEGG	bph:Bphy_5470.
PATRIC	19197752. VBIBurPhy25146_5757.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BURP8

Accession

Primary (citable) accession number: B2JNZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program