Reviewed, UniProtKB/Swiss-Prot B2JKI4 (ACEK_BURP8)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Bphy_0180

Organism

Burkholderia phymatum (strain DSM 17167 / STM815) [Complete proteome] [HAMAP]

Taxonomic identifier

391038 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 602	602	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_1000133261
Regions
	Nucleotide binding	327 – 333	7	ATP By similarity
Sites
	Active site	383	1	By similarity
	Binding site	348	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2JKI4-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 85980D507E54E44D	FASTA	602	69,768
	10 20 30 40 50 60 MNHFPKLLSS QIGFDVAETL LAGFDRHYCI FREAAIRAKN LFEAADWHGL QKLARERITS 70 80 90 100 110 120 YDERVRECIA KLEDEYDAEN IDDDVWQQIK LHYIGLLTTH RQPECAETFF NSVCCHILHR 130 140 150 160 170 180 SYFNNDFIFV RPAISTEYIE NDEPAAKPTY RAYYPGKDGL AVTLERIVTN FQLNPPFENL 190 200 210 220 230 240 TRDVQCVIQA LRDNFGTFNE APNFQIHVLS SLFFRNKAAY IIGRIINGDM MLPFALPVHH 250 260 270 280 290 300 VKPGLLALDA LLCKRDQLLI IFSFAHSYFL VDMEVPSAYV EFLGSILHGK PKAEIYTSVG 310 320 330 340 350 360 LQKQGKNLFY RDLLRHLKHS SDRFIIAPGI KGMVMLVFTL PSFPYVFKLI KDAFPPPKET 370 380 390 400 410 420 TREQVVGKYQ LVKRHDRLGR MADTLEYSSV ALPLSRLDDA LIRELEKEAP SMLEYEGDNL 430 440 450 460 470 480 VIRHVYIERR MVPLNIFLQN GTDADIEHGI REYGNAVKEL MQANIFPGDM LYKNFGVTRH 490 500 510 520 530 540 GRVVFYDYDE IEYLTDCNVR AVPPPRNEED EMSGEPWYSV GPHDIFPETY NTFLLGDPRV 550 560 570 580 590 600 RESFLKHHAD FFDPALWQKQ KDYILRGELP DFYPYDRSLR FSIRYPERFA ADHVTAATER AA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phymatum STM815." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Bruce D., Goodwin L., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Bacher J., Blanchard J., Cohan F., James E., Lawrence J., Lizotte-Waniewski M., Moulin L., Rainey P., Riley M., Souza V., Wertz J., Young P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001043 Genomic DNA. Translation: ACC69373.1.
RefSeq	YP_001856419.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6241682.
GenomeReviews	Gene locus Bphy_0180 in contig CP001043_GR.
KEGG	bph:Bphy_0180.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FYRDFLH.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_BURP8

Accession

Primary (citable) accession number: B2JKI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)