Reviewed, UniProtKB/Swiss-Prot B2JKH5 (BIOD_BURP8)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	Bphy_0171

Organism

Burkholderia phymatum (strain DSM 17167 / STM815) [Complete proteome] [HAMAP]

Taxonomic identifier

391038 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP cobalamin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: InterPro dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Dethiobiotin synthetase HAMAP MF_00336		PRO_1000119864
Regions
	Nucleotide binding	11 – 19	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2JKH5-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 3F101BB2DF5D9C2F	FASTA	239	25,193
	10 20 30 40 50 60 MSPALSLFVT GTDTEIGKTL VSAALLRGFV RDGLRAAAMK PIAAGATLVN GVLHNEDADQ 70 80 90 100 110 120 LDAAANVLLP PDIRTPFMLK EPAAPHIAAA HESVTLDMAR IVDAHEAALQ MADVVVVEGV 130 140 150 160 170 180 GGFRVPLTDS HDTADLAFAL NLPVVLVVGM RLGCISHALL TAEAIAARGL RVVGWVANRV 190 200 210 220 230 DPAMLFPDEN IDTLRDRLAR QYDAPLLGSV PHLSPASPDV AATHLDTTRL LHALRTAPR

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phymatum STM815." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Bruce D., Goodwin L., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Bacher J., Blanchard J., Cohan F., James E., Lawrence J., Lizotte-Waniewski M., Moulin L., Rainey P., Riley M., Souza V., Wertz J., Young P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001043 Genomic DNA. Translation: ACC69364.1.
RefSeq	YP_001856410.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6241673.
GenomeReviews	Gene locus Bphy_0171 in contig CP001043_GR.
KEGG	bph:Bphy_0171.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ETPEGMR.
Family and domain databases
HAMAP	MF_00336. [Tree]
InterPro	IPR004472. BioD_synth. IPR002586. CbiA_P_synth. [Graphical view]
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BURP8

Accession

Primary (citable) accession number: B2JKH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)