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B2JKH4

- BIOB_BURP8

UniProt

B2JKH4 - BIOB_BURP8

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Protein

Biotin synthase

Gene
bioB, Bphy_0170
Organism
Burkholderia phymatum (strain DSM 17167 / STM815)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi80 – 801Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi117 – 1171Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi148 – 1481Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi208 – 2081Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi280 – 2801Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBPHY391038:GI4Z-173-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Bphy_0170
OrganismiBurkholderia phymatum (strain DSM 17167 / STM815)
Taxonomic identifieri391038 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
ProteomesiUP000001192: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Biotin synthaseUniRule annotationPRO_0000381272Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi391038.Bphy_0170.

Structurei

3D structure databases

ProteinModelPortaliB2JKH4.
SMRiB2JKH4. Positions 26-333.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B2JKH4-1 [UniParc]FASTAAdd to Basket

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MSQIQTAPLP VMPSQAAAAA DAAASRWKVA DVAALYELPF NDLLFRAQQV    50
HREHFDANTV QLSTLLSIKT GGCEEDCAYC PQSVHHETGL KAEKLMEVEE 100
VLAAARVAKE NGATRFCMGA AWRNPKDRHL EPIKDMIRGV KSMGLETCVT 150
LGMLEAHQAQ ALREAGLDYY NHNLDTSPEF YGQIISTRTY QDRLDTLEHV 200
RDAGINVCCG GIVGLGESRR ERAGLIAQLA NMEPYPESVP INNLVQVEGT 250
PLTGTEALDP FEFVRTIAVA RITMPRAMVR LSAGREQMDE ALQAMCFLAG 300
ANSIFYGDQL LTTSNPQAEA DRKLLARLGI RAEAAQQMPA ERECSHGCEG 350
H 351
Length:351
Mass (Da):38,455
Last modified:June 10, 2008 - v1
Checksum:iE6B133485D7702E4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001043 Genomic DNA. Translation: ACC69363.1.
RefSeqiYP_001856409.1. NC_010622.1.

Genome annotation databases

EnsemblBacteriaiACC69363; ACC69363; Bphy_0170.
GeneIDi6241672.
KEGGibph:Bphy_0170.
PATRICi19186449. VBIBurPhy25146_0183.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001043 Genomic DNA. Translation: ACC69363.1 .
RefSeqi YP_001856409.1. NC_010622.1.

3D structure databases

ProteinModelPortali B2JKH4.
SMRi B2JKH4. Positions 26-333.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 391038.Bphy_0170.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACC69363 ; ACC69363 ; Bphy_0170 .
GeneIDi 6241672.
KEGGi bph:Bphy_0170.
PATRICi 19186449. VBIBurPhy25146_0183.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci BPHY391038:GI4Z-173-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17167 / STM815.

Entry informationi

Entry nameiBIOB_BURP8
AccessioniPrimary (citable) accession number: B2JKH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 10, 2008
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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