Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

B2JJY4

- DAPF_BURP8

UniProt

B2JJY4 - DAPF_BURP8

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Burkholderia phymatum (strain DSM 17167 / STM815)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131SubstrateUniRule annotation
    Binding sitei46 – 461SubstrateUniRule annotation
    Binding sitei66 – 661SubstrateUniRule annotation
    Active sitei75 – 751Proton donor/acceptorUniRule annotation
    Binding sitei166 – 1661SubstrateUniRule annotation
    Sitei168 – 1681Important for catalytic activityUniRule annotation
    Binding sitei199 – 1991SubstrateUniRule annotation
    Sitei217 – 2171Important for catalytic activityUniRule annotation
    Active sitei226 – 2261Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciBPHY391038:GI4Z-78-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:Bphy_0076
    OrganismiBurkholderia phymatum (strain DSM 17167 / STM815)
    Taxonomic identifieri391038 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
    ProteomesiUP000001192: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 286286Diaminopimelate epimerasePRO_1000099222Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi75 ↔ 226

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi391038.Bphy_0076.

    Structurei

    3D structure databases

    ProteinModelPortaliB2JJY4.
    SMRiB2JJY4. Positions 3-284.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni75 – 773Substrate bindingUniRule annotation
    Regioni217 – 2182Substrate bindingUniRule annotation
    Regioni227 – 2282Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220466.
    KOiK01778.
    OMAiKDITVMV.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2JJY4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLKFTKMHG AGNDFVVLDG YTQPLNLTET QVRALANRHF GVGADQLLVV    50
    EKPTVDGVDF RYRIFNCDGG EVEHCGNGAR CFVKFVRDSR LTDKRSVRVQ 100
    VQKGVITLTM QDNGEVVVDM GAPVFEPVQV PFDASGVEAR REGNDTLYAL 150
    EVNGETRWVS VVSMGNPHAV QVVDDVEAFP VLVDGPVIET HPRFPQRVNA 200
    GFMQIVGRSE VKLRVYERGA GETLACGTGA CAAVAAGIRR GLLDAPVKVH 250
    THGGTLTITW NGESASSLMM AGPAATVFEG EIELAD 286
    Length:286
    Mass (Da):30,870
    Last modified:June 10, 2008 - v1
    Checksum:i79FD072E1C848D9D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001043 Genomic DNA. Translation: ACC69271.1.
    RefSeqiWP_012399501.1. NC_010622.1.
    YP_001856317.1. NC_010622.1.

    Genome annotation databases

    EnsemblBacteriaiACC69271; ACC69271; Bphy_0076.
    GeneIDi6241580.
    KEGGibph:Bphy_0076.
    PATRICi19186239. VBIBurPhy25146_0079.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001043 Genomic DNA. Translation: ACC69271.1 .
    RefSeqi WP_012399501.1. NC_010622.1.
    YP_001856317.1. NC_010622.1.

    3D structure databases

    ProteinModelPortali B2JJY4.
    SMRi B2JJY4. Positions 3-284.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 391038.Bphy_0076.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACC69271 ; ACC69271 ; Bphy_0076 .
    GeneIDi 6241580.
    KEGGi bph:Bphy_0076.
    PATRICi 19186239. VBIBurPhy25146_0079.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220466.
    KOi K01778.
    OMAi KDITVMV.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci BPHY391038:GI4Z-78-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 17167 / STM815.

    Entry informationi

    Entry nameiDAPF_BURP8
    AccessioniPrimary (citable) accession number: B2JJY4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3