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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) (Burkholderia phymatum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei85SubstrateUniRule annotation1
Metal bindingi233ManganeseUniRule annotation1
Metal bindingi253Manganese; via tele nitrogenUniRule annotation1
Binding sitei275SubstrateUniRule annotation1
Active sitei277UniRule annotation1
Metal bindingi302ManganeseUniRule annotation1
Binding sitei398GTPUniRule annotation1
Binding sitei429GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi276 – 281GTPUniRule annotation6
Nucleotide bindingi526 – 529GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Bphy_0030
OrganismiParaburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) (Burkholderia phymatum)
Taxonomic identifieri391038 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeParaburkholderia
Proteomesi
  • UP000001192 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001250441 – 618Phosphoenolpyruvate carboxykinase [GTP]Add BLAST618

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi391038.Bphy_0030.

Structurei

3D structure databases

ProteinModelPortaliB2JJT8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni224 – 226Substrate bindingUniRule annotation3
Regioni396 – 398Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.
OrthoDBiPOG091H0B4F.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2JJT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNHPAFAAHS TLEAPAWVKH KKLIEWVQRV AALAKPERIV WCDGSQEEYD
60 70 80 90 100
RLTAQMVAAG TLRRLNPAKR PNSYLACSDP SDVARVEDRT FICSQRREDA
110 120 130 140 150
GPTNNWIAPA EMRATLDGLF DGAMRGRTMY VVPFSMGPLG SPIAHIGVEL
160 170 180 190 200
SDSPYVVSNM RIMTRMGRKV YDVLGEDGEF VPCVHSVGAP LAAGQKDVAW
210 220 230 240 250
PCNDTKYIVH FPESREIWSY GSGYGGNALL GKKCFALRIA STMGRAEGWL
260 270 280 290 300
AEHMLVLGVT SPEGKKHHVA AAFPSACGKT NFAMLIPPQG MNGWKITTIG
310 320 330 340 350
DDIAWIKPGK DGRLYAINPE AGYFGVAPGT SEKTNFNAMA TLKENVIFTN
360 370 380 390 400
VALTDDGDVW WEGMTDEPPA HLIDWQGRDW TPAIAKETGR KAAHPNARFT
410 420 430 440 450
APASQCPSID ADWENPAGVP IDAFVFGGRR STTVPLVTEA RDWVEGVYMA
460 470 480 490 500
ATMGSETTAA AAGQQGVVRR DPFAMLPFCG YNMSDYFAHW LKTGVKLEQM
510 520 530 540 550
SVRTPKIFCV NWFRKGDDGK FVWPGFGENM RVLRWMVDRI EGKAKGEEHA
560 570 580 590 600
FGVSPRYDDL DWSGLDFTRE QFEQVISVDD AAWRKELALH SELFDTLKQG
610
LPVALQDTRS ALEKKLTA
Length:618
Mass (Da):68,288
Last modified:June 10, 2008 - v1
Checksum:i9C8B5F60E4871ABF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001043 Genomic DNA. Translation: ACC69225.1.
RefSeqiWP_012399456.1. NC_010622.1.

Genome annotation databases

EnsemblBacteriaiACC69225; ACC69225; Bphy_0030.
GeneIDi27739702.
KEGGibph:Bphy_0030.
PATRICi19186137. VBIBurPhy25146_0029.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001043 Genomic DNA. Translation: ACC69225.1.
RefSeqiWP_012399456.1. NC_010622.1.

3D structure databases

ProteinModelPortaliB2JJT8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391038.Bphy_0030.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC69225; ACC69225; Bphy_0030.
GeneIDi27739702.
KEGGibph:Bphy_0030.
PATRICi19186137. VBIBurPhy25146_0029.

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.
OrthoDBiPOG091H0B4F.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_PARP8
AccessioniPrimary (citable) accession number: B2JJT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: November 2, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.