Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

B2JJT8

- PCKG_BURP8

UniProt

B2JJT8 - PCKG_BURP8

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Burkholderia phymatum (strain DSM 17167 / STM815)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei85 – 851SubstrateUniRule annotation
    Binding sitei226 – 2261Substrate; via amide nitrogenUniRule annotation
    Metal bindingi233 – 2331ManganeseUniRule annotation
    Binding sitei233 – 2331SubstrateUniRule annotation
    Metal bindingi253 – 2531Manganese; via tele nitrogenUniRule annotation
    Binding sitei275 – 2751SubstrateUniRule annotation
    Active sitei277 – 2771UniRule annotation
    Metal bindingi302 – 3021ManganeseUniRule annotation
    Binding sitei398 – 3981GTPUniRule annotation
    Binding sitei429 – 4291GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi276 – 2816GTPUniRule annotation
    Nucleotide bindingi526 – 5294GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBPHY391038:GI4Z-31-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:Bphy_0030
    OrganismiBurkholderia phymatum (strain DSM 17167 / STM815)
    Taxonomic identifieri391038 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
    ProteomesiUP000001192: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 618618Phosphoenolpyruvate carboxykinase [GTP]PRO_1000125044Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi391038.Bphy_0030.

    Structurei

    3D structure databases

    ProteinModelPortaliB2JJT8.
    SMRiB2JJT8. Positions 17-617.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni396 – 3983Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiWDNPAGV.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2JJT8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNHPAFAAHS TLEAPAWVKH KKLIEWVQRV AALAKPERIV WCDGSQEEYD    50
    RLTAQMVAAG TLRRLNPAKR PNSYLACSDP SDVARVEDRT FICSQRREDA 100
    GPTNNWIAPA EMRATLDGLF DGAMRGRTMY VVPFSMGPLG SPIAHIGVEL 150
    SDSPYVVSNM RIMTRMGRKV YDVLGEDGEF VPCVHSVGAP LAAGQKDVAW 200
    PCNDTKYIVH FPESREIWSY GSGYGGNALL GKKCFALRIA STMGRAEGWL 250
    AEHMLVLGVT SPEGKKHHVA AAFPSACGKT NFAMLIPPQG MNGWKITTIG 300
    DDIAWIKPGK DGRLYAINPE AGYFGVAPGT SEKTNFNAMA TLKENVIFTN 350
    VALTDDGDVW WEGMTDEPPA HLIDWQGRDW TPAIAKETGR KAAHPNARFT 400
    APASQCPSID ADWENPAGVP IDAFVFGGRR STTVPLVTEA RDWVEGVYMA 450
    ATMGSETTAA AAGQQGVVRR DPFAMLPFCG YNMSDYFAHW LKTGVKLEQM 500
    SVRTPKIFCV NWFRKGDDGK FVWPGFGENM RVLRWMVDRI EGKAKGEEHA 550
    FGVSPRYDDL DWSGLDFTRE QFEQVISVDD AAWRKELALH SELFDTLKQG 600
    LPVALQDTRS ALEKKLTA 618
    Length:618
    Mass (Da):68,288
    Last modified:June 10, 2008 - v1
    Checksum:i9C8B5F60E4871ABF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001043 Genomic DNA. Translation: ACC69225.1.
    RefSeqiWP_012399456.1. NC_010622.1.
    YP_001856271.1. NC_010622.1.

    Genome annotation databases

    EnsemblBacteriaiACC69225; ACC69225; Bphy_0030.
    GeneIDi6241534.
    KEGGibph:Bphy_0030.
    PATRICi19186137. VBIBurPhy25146_0029.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001043 Genomic DNA. Translation: ACC69225.1 .
    RefSeqi WP_012399456.1. NC_010622.1.
    YP_001856271.1. NC_010622.1.

    3D structure databases

    ProteinModelPortali B2JJT8.
    SMRi B2JJT8. Positions 17-617.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 391038.Bphy_0030.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACC69225 ; ACC69225 ; Bphy_0030 .
    GeneIDi 6241534.
    KEGGi bph:Bphy_0030.
    PATRICi 19186137. VBIBurPhy25146_0029.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi WDNPAGV.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci BPHY391038:GI4Z-31-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 17167 / STM815.

    Entry informationi

    Entry nameiPCKG_BURP8
    AccessioniPrimary (citable) accession number: B2JJT8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3