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B2JJT8

- PCKG_BURP8

UniProt

B2JJT8 - PCKG_BURP8

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Burkholderia phymatum (strain DSM 17167 / STM815)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn(2+) ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851SubstrateUniRule annotation
Binding sitei226 – 2261Substrate; via amide nitrogenUniRule annotation
Metal bindingi233 – 2331ManganeseUniRule annotation
Binding sitei233 – 2331SubstrateUniRule annotation
Metal bindingi253 – 2531Manganese; via tele nitrogenUniRule annotation
Binding sitei275 – 2751SubstrateUniRule annotation
Active sitei277 – 2771UniRule annotation
Metal bindingi302 – 3021ManganeseUniRule annotation
Binding sitei398 – 3981GTPUniRule annotation
Binding sitei429 – 4291GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi276 – 2816GTPUniRule annotation
Nucleotide bindingi526 – 5294GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBPHY391038:GI4Z-31-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Bphy_0030
OrganismiBurkholderia phymatum (strain DSM 17167 / STM815)
Taxonomic identifieri391038 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
ProteomesiUP000001192: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 618618Phosphoenolpyruvate carboxykinase [GTP]PRO_1000125044Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi391038.Bphy_0030.

Structurei

3D structure databases

ProteinModelPortaliB2JJT8.
SMRiB2JJT8. Positions 17-617.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni396 – 3983Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWDNPAGV.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2JJT8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNHPAFAAHS TLEAPAWVKH KKLIEWVQRV AALAKPERIV WCDGSQEEYD
60 70 80 90 100
RLTAQMVAAG TLRRLNPAKR PNSYLACSDP SDVARVEDRT FICSQRREDA
110 120 130 140 150
GPTNNWIAPA EMRATLDGLF DGAMRGRTMY VVPFSMGPLG SPIAHIGVEL
160 170 180 190 200
SDSPYVVSNM RIMTRMGRKV YDVLGEDGEF VPCVHSVGAP LAAGQKDVAW
210 220 230 240 250
PCNDTKYIVH FPESREIWSY GSGYGGNALL GKKCFALRIA STMGRAEGWL
260 270 280 290 300
AEHMLVLGVT SPEGKKHHVA AAFPSACGKT NFAMLIPPQG MNGWKITTIG
310 320 330 340 350
DDIAWIKPGK DGRLYAINPE AGYFGVAPGT SEKTNFNAMA TLKENVIFTN
360 370 380 390 400
VALTDDGDVW WEGMTDEPPA HLIDWQGRDW TPAIAKETGR KAAHPNARFT
410 420 430 440 450
APASQCPSID ADWENPAGVP IDAFVFGGRR STTVPLVTEA RDWVEGVYMA
460 470 480 490 500
ATMGSETTAA AAGQQGVVRR DPFAMLPFCG YNMSDYFAHW LKTGVKLEQM
510 520 530 540 550
SVRTPKIFCV NWFRKGDDGK FVWPGFGENM RVLRWMVDRI EGKAKGEEHA
560 570 580 590 600
FGVSPRYDDL DWSGLDFTRE QFEQVISVDD AAWRKELALH SELFDTLKQG
610
LPVALQDTRS ALEKKLTA
Length:618
Mass (Da):68,288
Last modified:June 10, 2008 - v1
Checksum:i9C8B5F60E4871ABF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001043 Genomic DNA. Translation: ACC69225.1.
RefSeqiWP_012399456.1. NC_010622.1.
YP_001856271.1. NC_010622.1.

Genome annotation databases

EnsemblBacteriaiACC69225; ACC69225; Bphy_0030.
GeneIDi6241534.
KEGGibph:Bphy_0030.
PATRICi19186137. VBIBurPhy25146_0029.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001043 Genomic DNA. Translation: ACC69225.1 .
RefSeqi WP_012399456.1. NC_010622.1.
YP_001856271.1. NC_010622.1.

3D structure databases

ProteinModelPortali B2JJT8.
SMRi B2JJT8. Positions 17-617.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 391038.Bphy_0030.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACC69225 ; ACC69225 ; Bphy_0030 .
GeneIDi 6241534.
KEGGi bph:Bphy_0030.
PATRICi 19186137. VBIBurPhy25146_0029.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi WDNPAGV.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci BPHY391038:GI4Z-31-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17167 / STM815.

Entry informationi

Entry nameiPCKG_BURP8
AccessioniPrimary (citable) accession number: B2JJT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: November 26, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3