B2JJ71 (GCST_BURP8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	Bphy_3003

Organism

Burkholderia phymatum (strain DSM 17167 / STM815) [Complete proteome] [HAMAP]

Taxonomic identifier

391038 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Aminomethyltransferase HAMAP MF_00259		PRO_1000114084

Sequences

	Sequence		Length	Mass (Da)
	B2JJ71 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 30C2FA34C7A2BDB0	FASTA	372	40,311
	10 20 30 40 50 60 MTELKHTPLH ATHRALNARM VDFGGWDMPV NYGSQIEEHR AVRTDAGMFD VSHMCVVDFT 70 80 90 100 110 120 GERVRAFFER ALANNVGKLQ TAGKALYSCL LNPQGGVIDD LIVYYFGEDH FRVVVNAGTA 130 140 150 160 170 180 EKDIAWFNKL NDEEGFGLTI TPRRDYAIVA VQGPNAREKV WATVPHARAA SEALKPFNAA 190 200 210 220 230 240 RVADTPFGEL TVARTGYTGE DGFEIIVPAA HVEALWNALQ AQGVRPAGLG ARDTLRLEAG 250 260 270 280 290 300 MNLYGQDMDD DVSPLDAGLA WTVDLSAPRE FIGRAKLEAD GSKQSFVGLI LHKDNGKAAG 310 320 330 340 350 360 VLRAHQKVVT PNGEGEITSG TFSPTMQESI AFARVPKGVQ PGDVVHVQIR DKALPASVVK 370 LPFVRNGKVL VG

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phymatum STM815." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Bruce D., Goodwin L., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Bacher J., Blanchard J., Cohan F., James E., Lawrence J., Lizotte-Waniewski M., Moulin L., Rainey P., Riley M., Souza V., Wertz J., Young P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17167 / STM815.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001043 Genomic DNA. Translation: ACC72173.1.
RefSeq	YP_001859219.1. NC_010622.1.
3D structure databases
ProteinModelPortal	B2JJ71.
SMR	B2JJ71. Positions 6-372.
ModBase	Search...
Protein-protein interaction databases
STRING	B2JJ71.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6244484.
GenomeReviews	Gene locus Bphy_3003 in contig CP001043_GR.
KEGG	bph:Bphy_3003.
PATRIC	19192592. VBIBurPhy25146_3189.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG299834.
OMA	KALYGGM.
ProtClustDB	PRK00389.
Family and domain databases
HAMAP	MF_00259. GcvT. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. [Graphical view]
KO	K00605.
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. GcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_BURP8

Accession

Primary (citable) accession number: B2JJ71

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program