B2JIQ8 (SYE_BURP8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Bphy_1377

Organism

Burkholderia phymatum (strain DSM 17167 / STM815) [Complete proteome] [HAMAP]

Taxonomic identifier

391038 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_1000090059
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00022_B
	Motif	243 – 247	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	246	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2JIQ8 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: F5DFB300A8AEA085	FASTA	469	52,377
	10 20 30 40 50 60 MTTPVRTRFA PSPTGFIHLG NIRSALYPWA FARKSKGVFV LRIEDTDVER STEQSVDAIL 70 80 90 100 110 120 EGMQWLGLDY DEGPFYQMQR MDRYREVLKH MQEQGLVYPC YMSTEELDAL RERQRASGEK 130 140 150 160 170 180 PRYDGTWRPE PGKVLPTPPA GVQPVLRFRN PLTGVVAWDD AVKGRVEISN EELDDLVIAR 190 200 210 220 230 240 PDGTPTYNFC VVVDDLDMKI THVIRGDDHV NNTPRQINIL RALGGEPPVY AHLPTVLNEQ 250 260 270 280 290 300 GEKMSKRHGA MSVMGYRDAG YLPEAVLNYL ARLGWSHGDA EIFSREQLIE WFDLEHLGKS 310 320 330 340 350 360 PAQYDHNKLN WLNNHYIKEA DNARLADLSK PFFAALGIDE PTLAKGADLV SVIALMKDRA 370 380 390 400 410 420 STVKEVVDNA AMFYREPNPD ADALAQHVTD AVRPALADLA AALKACEWTK EGIAAALKAT 430 440 450 460 LGAHKLKMPQ LAMPVRVLVA GTTHTPSIDS VLMLFGRDVV VSRIEKAVV

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phymatum STM815." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Bruce D., Goodwin L., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Bacher J., Blanchard J., Cohan F., James E., Lawrence J., Lizotte-Waniewski M., Moulin L., Rainey P., Riley M., Souza V., Wertz J., Young P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17167 / STM815.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001043 Genomic DNA. Translation: ACC70559.1.
RefSeq	YP_001857605.1. NC_010622.1.
3D structure databases
ProteinModelPortal	B2JIQ8.
ModBase	Search...
Protein-protein interaction databases
STRING	B2JIQ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6244651.
GenomeReviews	Gene locus Bphy_1377 in contig CP001043_GR.
KEGG	bph:Bphy_1377.
PATRIC	19189074. VBIBurPhy25146_1464.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	ANFNDES.
ProtClustDB	PRK01406.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_BURP8

Accession

Primary (citable) accession number: B2JIQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program