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B2JIP4 (SAHH_BURP8) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Adenosylhomocysteinase
EC=3.3.1.1
Alternative name(s):
S-adenosyl-L-homocysteine hydrolase
Short name=AdoHcyase
Gene names
Name:ahcY
Ordered Locus Names:Bphy_2918
OrganismBurkholderia phymatum (strain DSM 17167 / STM815) [Complete proteome] [HAMAP]
Taxonomic identifier391038 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length473 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine By similarity. HAMAP MF_00563

Catalytic activity

S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563

Cofactor

Binds 1 NAD per subunit By similarity. HAMAP MF_00563

Pathway

Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563

Subcellular location

Cytoplasm By similarity HAMAP MF_00563.

Sequence similarities

Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processone-carbon metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionadenosylhomocysteinase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 473473Adenosylhomocysteinase HAMAP MF_00563
PRO_1000129273

Regions

Nucleotide binding200 – 2023NAD By similarity
Nucleotide binding263 – 2686NAD By similarity
Nucleotide binding342 – 3443NAD By similarity

Sites

Binding site641Substrate By similarity
Binding site1391Substrate By similarity
Binding site1991Substrate By similarity
Binding site2291Substrate By similarity
Binding site2331Substrate By similarity
Binding site2341NAD By similarity
Binding site2861NAD By similarity
Binding site3211NAD By similarity
Binding site3871NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
B2JIP4 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 25B794C3CE5D44FF

FASTA47352,204
        10         20         30         40         50         60 
MNAAVIDSKH SQDFIVADLS LADWGRKELD IAETEMPGLV QIREEYKAQQ PLKGARVAGS 

        70         80         90        100        110        120 
LHMTIQTGVL IETLKALGAD VRWASCNIFS TQDHAAAAIA KGGTPVFAFK GESLDEYWEF 

       130        140        150        160        170        180 
SHRIFEWPNG EFANMILDDG GDATLLLILG SKAEKDRSVI SKPTNEEEVA LYKSIARHLD 

       190        200        210        220        230        240 
ADPVWYSTRL AHIKGVTEET TTGVHRLYQM EKEGRLPFPA INVNDSVTKS KFDNLYGCRE 

       250        260        270        280        290        300 
SLVDGIKRAT DVMIAGKIAV VAGYGDVGKG CAQSLRGLGA TVWVTEIDPI CALQAAMEGY 

       310        320        330        340        350        360 
RVVTMEYAAD KADIFVTATG NYHVIGHDHM KAMRHNAIVC NIGHFDSEID VASTRQYTWE 

       370        380        390        400        410        420 
NIKPQVDHII FPDGKRVILL AEGRLVNLGC ATGHPSFVMS NSFTNQTLAQ IELFVEGSKY 

       430        440        450        460        470 
ENKVYVLPKQ LDEKVARLHL ARIGANLTEL TSEQAGYIGV DKNGPFKPNH YRY

« Hide

References

[1]"Complete sequence of chromosome 1 of Burkholderia phymatum STM815."
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Bruce D., Goodwin L., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Bacher J., Blanchard J., Cohan F., James E., Lawrence J., Lizotte-Waniewski M., Moulin L., Rainey P., Riley M., Souza V., Wertz J., Young P.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17167 / STM815.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001043 Genomic DNA. Translation: ACC72090.1.
RefSeqYP_001859136.1. NC_010622.1.

3D structure databases

ProteinModelPortalB2JIP4.
SMRB2JIP4. Positions 12-473.
ModBaseSearch...

Protein-protein interaction databases

STRINGB2JIP4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6244401.
GenomeReviewsGene locus Bphy_2918 in contig CP001043_GR.
KEGGbph:Bphy_2918.
PATRIC19192404. VBIBurPhy25146_3096.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG352029.
OMASAQVWVT.
ProtClustDBPRK05476.

Family and domain databases

HAMAPMF_00563. AdoHcyase.
[Tree]
InterProIPR000043. Adenosylhomocysteinase.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
[Graphical view]
KOK01251.
PANTHERPTHR23420. Ad_hcy_hydrolase. 1 hit.
PfamPF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
[Graphical view]
PIRSFPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTSM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
[Graphical view]
TIGRFAMsTIGR00936. AhcY. 1 hit.
PROSITEPS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSAHH_BURP8
AccessionPrimary (citable) accession number: B2JIP4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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