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Protein

Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase

Gene

Bphy_1374

Organism
Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) (Burkholderia phymatum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions (By similarity).By similarity

Catalytic activityi

4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate.
Oxaloacetate = pyruvate + CO2.

Cofactori

a divalent metal cationBy similarityNote: Divalent metal cation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102SubstrateBy similarity1
Metal bindingi103Divalent metal cationBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (EC:4.1.3.17)
Short name:
HMG aldolase
Alternative name(s):
Oxaloacetate decarboxylase (EC:4.1.1.3)
Short name:
OAA decarboxylase
Regulator of ribonuclease activity homolog
RraA-like protein
Gene namesi
Ordered Locus Names:Bphy_1374
OrganismiParaburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) (Burkholderia phymatum)
Taxonomic identifieri391038 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeParaburkholderia
Proteomesi
  • UP000001192 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001255951 – 164Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolaseAdd BLAST164

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi391038.Bphy_1374.

Structurei

3D structure databases

ProteinModelPortaliB2JIG2.
SMRiB2JIG2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni80 – 83Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the class II aldolase/RraA-like family.Curated

Phylogenomic databases

eggNOGiENOG4108YYX. Bacteria.
COG0684. LUCA.
HOGENOMiHOG000252803.
KOiK02553.
OMAiRSCDTQF.
OrthoDBiPOG091H03SM.

Family and domain databases

CDDicd16841. RraA_family. 1 hit.
Gene3Di3.50.30.40. 1 hit.
InterProiView protein in InterPro
IPR010203. RraA.
IPR005493. RraA/RraA-like.
IPR036704. RraA/RraA-like_sf.
PfamiView protein in Pfam
PF03737. RraA-like. 1 hit.
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.

Sequencei

Sequence statusi: Complete.

B2JIG2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFATADLCD AHEDQLTAGT LRVLDPVFSR FGRASRFGGE AVTLKVFEDN
60 70 80 90 100
TLVRATLEEK GVARVLVVDG GGSLRCALVG GNLGKLGEKN GWAGIVVFGC
110 120 130 140 150
VRDTLELNEC NLGIIALATH PQRSQKRGGG ERDVSLRLPG SVVRPGEWIY
160
ADSDGVLVSS APLV
Length:164
Mass (Da):17,365
Last modified:June 10, 2008 - v1
Checksum:iFBF4CAC27371131B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001043 Genomic DNA. Translation: ACC70556.1.
RefSeqiWP_012400770.1. NC_010622.1.

Genome annotation databases

EnsemblBacteriaiACC70556; ACC70556; Bphy_1374.
GeneIDi27741056.
KEGGibph:Bphy_1374.

Similar proteinsi

Entry informationi

Entry nameiRRAAH_PARP8
AccessioniPrimary (citable) accession number: B2JIG2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: October 25, 2017
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families