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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Burkholderia phymatum (strain DSM 17167 / STM815)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.UniRule annotation

Pathway:ilipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH), UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciBPHY391038:GI4Z-1356-MONOMER.
UniPathwayiUPA00359; UER00481.

Protein family/group databases

CAZyiGT19. Glycosyltransferase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthaseUniRule annotation (EC:2.4.1.182UniRule annotation)
Gene namesi
Name:lpxBUniRule annotation
Ordered Locus Names:Bphy_1325
OrganismiBurkholderia phymatum (strain DSM 17167 / STM815)
Taxonomic identifieri391038 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
ProteomesiUP000001192 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 389389Lipid-A-disaccharide synthasePRO_1000191467Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi391038.Bphy_1325.

Structurei

3D structure databases

ProteinModelPortaliB2JIB3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0763.
HOGENOMiHOG000018003.
KOiK00748.
OMAiTVHFVCP.
OrthoDBiEOG6FBWZR.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.

Sequencei

Sequence statusi: Complete.

B2JIB3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLPTSPLRL AMVAGEPSGD LLAASMLDGL AARLPDTTQY FGIGGPRMIA
60 70 80 90 100
KGFDAHFAME KLSVRGYVEA LKHVPEILGI RNELKRQLLA EPPSAFIGVD
110 120 130 140 150
APDFNFGLEH PLRDAGIPTI HFVCPSIWAW RGGRIKKIVK AVDHMLCVFP
160 170 180 190 200
FEKALLEKSG VTATYVGHPL ADEIPLEPDT AGARLELGLP ESGPVIAVLP
210 220 230 240 250
GSRRSEIALI GPTFFDAMEL MLQREPGVRF VMPAATPALR ELLKPLVDAH
260 270 280 290 300
ANLPLTLTDG NAQRAMTAAD AILVKSGTVT LEAALLKKPM VISYKVPWLT
310 320 330 340 350
GQIMRRQGYL PYVGLPNILA GRFVVPEILQ HFATPEALAD ATLTQLRDDA
360 370 380
NRRTLTEIFT EMHHVLKQNT AQRAAEAVVG VIEARRGRP
Length:389
Mass (Da):42,289
Last modified:June 10, 2008 - v1
Checksum:i8EBA7A370C6313E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001043 Genomic DNA. Translation: ACC70507.1.
RefSeqiWP_012400721.1. NC_010622.1.

Genome annotation databases

EnsemblBacteriaiACC70507; ACC70507; Bphy_1325.
KEGGibph:Bphy_1325.
PATRICi19188952. VBIBurPhy25146_1408.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001043 Genomic DNA. Translation: ACC70507.1.
RefSeqiWP_012400721.1. NC_010622.1.

3D structure databases

ProteinModelPortaliB2JIB3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391038.Bphy_1325.

Protein family/group databases

CAZyiGT19. Glycosyltransferase Family 19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC70507; ACC70507; Bphy_1325.
KEGGibph:Bphy_1325.
PATRICi19188952. VBIBurPhy25146_1408.

Phylogenomic databases

eggNOGiCOG0763.
HOGENOMiHOG000018003.
KOiK00748.
OMAiTVHFVCP.
OrthoDBiEOG6FBWZR.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00481.
BioCyciBPHY391038:GI4Z-1356-MONOMER.

Family and domain databases

HAMAPiMF_00392. LpxB.
InterProiIPR003835. Glyco_trans_19.
[Graphical view]
PfamiPF02684. LpxB. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00215. lpxB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17167 / STM815.

Entry informationi

Entry nameiLPXB_BURP8
AccessioniPrimary (citable) accession number: B2JIB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: July 22, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.