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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) (Burkholderia phymatum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciBPHY391038:G1GBS-1364-MONOMER
UniPathwayiUPA00359; UER00481

Protein family/group databases

CAZyiGT19 Glycosyltransferase Family 19

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthaseUniRule annotation (EC:2.4.1.182UniRule annotation)
Gene namesi
Name:lpxBUniRule annotation
Ordered Locus Names:Bphy_1325
OrganismiParaburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) (Burkholderia phymatum)
Taxonomic identifieri391038 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeParaburkholderia
Proteomesi
  • UP000001192 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001914671 – 389Lipid-A-disaccharide synthaseAdd BLAST389

Interactioni

Protein-protein interaction databases

STRINGi391038.Bphy_1325

Structurei

3D structure databases

ProteinModelPortaliB2JIB3
SMRiB2JIB3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D0V Bacteria
COG0763 LUCA
HOGENOMiHOG000018003
KOiK00748
OMAiPTVWAWR
OrthoDBiPOG091H01NW

Family and domain databases

HAMAPiMF_00392 LpxB, 1 hit
InterProiView protein in InterPro
IPR003835 Glyco_trans_19
PANTHERiPTHR30372 PTHR30372, 1 hit
PfamiView protein in Pfam
PF02684 LpxB, 1 hit
TIGRFAMsiTIGR00215 lpxB, 1 hit

Sequencei

Sequence statusi: Complete.

B2JIB3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLPTSPLRL AMVAGEPSGD LLAASMLDGL AARLPDTTQY FGIGGPRMIA
60 70 80 90 100
KGFDAHFAME KLSVRGYVEA LKHVPEILGI RNELKRQLLA EPPSAFIGVD
110 120 130 140 150
APDFNFGLEH PLRDAGIPTI HFVCPSIWAW RGGRIKKIVK AVDHMLCVFP
160 170 180 190 200
FEKALLEKSG VTATYVGHPL ADEIPLEPDT AGARLELGLP ESGPVIAVLP
210 220 230 240 250
GSRRSEIALI GPTFFDAMEL MLQREPGVRF VMPAATPALR ELLKPLVDAH
260 270 280 290 300
ANLPLTLTDG NAQRAMTAAD AILVKSGTVT LEAALLKKPM VISYKVPWLT
310 320 330 340 350
GQIMRRQGYL PYVGLPNILA GRFVVPEILQ HFATPEALAD ATLTQLRDDA
360 370 380
NRRTLTEIFT EMHHVLKQNT AQRAAEAVVG VIEARRGRP
Length:389
Mass (Da):42,289
Last modified:June 10, 2008 - v1
Checksum:i8EBA7A370C6313E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001043 Genomic DNA Translation: ACC70507.1
RefSeqiWP_012400721.1, NC_010622.1

Genome annotation databases

EnsemblBacteriaiACC70507; ACC70507; Bphy_1325
GeneIDi27741007
KEGGibph:Bphy_1325

Similar proteinsi

Entry informationi

Entry nameiLPXB_PARP8
AccessioniPrimary (citable) accession number: B2JIB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: May 23, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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