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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Burkholderia phymatum (strain DSM 17167 / STM815)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei627 – 6271ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBPHY391038:GI4Z-2640-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Bphy_2588
OrganismiBurkholderia phymatum (strain DSM 17167 / STM815)
Taxonomic identifieri391038 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
ProteomesiUP000001192 Componenti: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 864864Leucine--tRNA ligasePRO_1000091300Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi391038.Bphy_2588.

Structurei

3D structure databases

ProteinModelPortaliB2JGX6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi624 – 6285"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2JGX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHEKYVPSDV ESAAQGQWRA TDAYKTTETA DKPKFYCVSM LPYPSGKLHM
60 70 80 90 100
GHVRNYTIND VMYRYLRMNG YNTLMPMGWD AFGMPAENAA MANGVPPAKW
110 120 130 140 150
TYDNIAYMKK QMQSMGLAID WSREVATCSP DYYKWNQWIF LKMLEKGIAY
160 170 180 190 200
KKTGTVNWDP VDQTVLANEQ VIDGRGWRSG ALVEKREIPM YYMRITQYAD
210 220 230 240 250
ELLNDLDGLG WPERVKVMQQ NWIGKSFGVN FGFPYELDGE QKLLRVFTTR
260 270 280 290 300
ADTIMGVTFC AVAAEHPLAT RLAQGRPDLQ SFIDECKHGG VAEADMATME
310 320 330 340 350
KKGMATGFFV THPLTQEKVE VWIGNYVLMS YGEGAVMGVP AHDERDFAFV
360 370 380 390 400
KKYGIPVKQV VAVEGETFST DAWQESYGDK ENGVLINSGK YDGLKYGEAV
410 420 430 440 450
DAIAADLKAL GLGEKQVTWR LRDWGISRQR YWGTPIPIIH CPSCGDVPVP
460 470 480 490 500
EKDLPVVLPE DLVPDGTGNP LAKSEAFLNC TCPTCGAAAK RETDTMDTFV
510 520 530 540 550
DSSWYFYRYA APDAKTMVDA RTDYWMPMDQ YIGGIEHAIL HLLYSRFWAK
560 570 580 590 600
VCRDLGIVKF GEPAKNLLTQ GMVLNETYYR ENDAGKKTWY NPADVTVTHD
610 620 630 640 650
DKGRPVGATL NADGQPVVLG GVEKMSKSKN NGVDPQVLID QYGADTARLF
660 670 680 690 700
TMFAAPPEQQ LEWSGAGVEG ASRFLRRVWA FGQANETALT ERAPFDAAKL
710 720 730 740 750
ADTEKTLRRE IYSVLKQADF DYQRLQYNTV VSAAMKMLNA IEGAKGASAA
760 770 780 790 800
VLRETYGVLL RVLYPVVPHV TFQLWRELGY EGEFGSLLDA PWPKVDEKAL
810 820 830 840 850
EQSEIELVLQ VNGKVRGAVT VAKDASREAI EAVALAHEMF AKFSEGKPAK
860
KVIVVPGRLV NVVV
Length:864
Mass (Da):96,522
Last modified:June 9, 2008 - v1
Checksum:i4D68B9ABCB2DFD12
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001043 Genomic DNA. Translation: ACC71760.1.
RefSeqiYP_001858806.1. NC_010622.1.

Genome annotation databases

EnsemblBacteriaiACC71760; ACC71760; Bphy_2588.
KEGGibph:Bphy_2588.
PATRICi19191671. VBIBurPhy25146_2746.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001043 Genomic DNA. Translation: ACC71760.1.
RefSeqiYP_001858806.1. NC_010622.1.

3D structure databases

ProteinModelPortaliB2JGX6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391038.Bphy_2588.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC71760; ACC71760; Bphy_2588.
KEGGibph:Bphy_2588.
PATRICi19191671. VBIBurPhy25146_2746.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciBPHY391038:GI4Z-2640-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17167 / STM815.

Entry informationi

Entry nameiSYL_BURP8
AccessioniPrimary (citable) accession number: B2JGX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2009
Last sequence update: June 9, 2008
Last modified: March 31, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.