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B2JGX6 (SYL_BURP8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Bphy_2588
OrganismBurkholderia phymatum (strain DSM 17167 / STM815) [Complete proteome] [HAMAP]
Taxonomic identifier391038 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia

Protein attributes

Sequence length864 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 864864Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091300

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif624 – 6285"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6271ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B2JGX6 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 4D68B9ABCB2DFD12

FASTA86496,522
        10         20         30         40         50         60 
MHEKYVPSDV ESAAQGQWRA TDAYKTTETA DKPKFYCVSM LPYPSGKLHM GHVRNYTIND 

        70         80         90        100        110        120 
VMYRYLRMNG YNTLMPMGWD AFGMPAENAA MANGVPPAKW TYDNIAYMKK QMQSMGLAID 

       130        140        150        160        170        180 
WSREVATCSP DYYKWNQWIF LKMLEKGIAY KKTGTVNWDP VDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALVEKREIPM YYMRITQYAD ELLNDLDGLG WPERVKVMQQ NWIGKSFGVN FGFPYELDGE 

       250        260        270        280        290        300 
QKLLRVFTTR ADTIMGVTFC AVAAEHPLAT RLAQGRPDLQ SFIDECKHGG VAEADMATME 

       310        320        330        340        350        360 
KKGMATGFFV THPLTQEKVE VWIGNYVLMS YGEGAVMGVP AHDERDFAFV KKYGIPVKQV 

       370        380        390        400        410        420 
VAVEGETFST DAWQESYGDK ENGVLINSGK YDGLKYGEAV DAIAADLKAL GLGEKQVTWR 

       430        440        450        460        470        480 
LRDWGISRQR YWGTPIPIIH CPSCGDVPVP EKDLPVVLPE DLVPDGTGNP LAKSEAFLNC 

       490        500        510        520        530        540 
TCPTCGAAAK RETDTMDTFV DSSWYFYRYA APDAKTMVDA RTDYWMPMDQ YIGGIEHAIL 

       550        560        570        580        590        600 
HLLYSRFWAK VCRDLGIVKF GEPAKNLLTQ GMVLNETYYR ENDAGKKTWY NPADVTVTHD 

       610        620        630        640        650        660 
DKGRPVGATL NADGQPVVLG GVEKMSKSKN NGVDPQVLID QYGADTARLF TMFAAPPEQQ 

       670        680        690        700        710        720 
LEWSGAGVEG ASRFLRRVWA FGQANETALT ERAPFDAAKL ADTEKTLRRE IYSVLKQADF 

       730        740        750        760        770        780 
DYQRLQYNTV VSAAMKMLNA IEGAKGASAA VLRETYGVLL RVLYPVVPHV TFQLWRELGY 

       790        800        810        820        830        840 
EGEFGSLLDA PWPKVDEKAL EQSEIELVLQ VNGKVRGAVT VAKDASREAI EAVALAHEMF 

       850        860 
AKFSEGKPAK KVIVVPGRLV NVVV 

« Hide

References

[1]"Complete sequence of chromosome 1 of Burkholderia phymatum STM815."
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Bruce D., Goodwin L., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Mikhailova N., Bacher J., Blanchard J., Cohan F., James E., Lawrence J., Lizotte-Waniewski M., Moulin L., Rainey P., Riley M., Souza V., Wertz J., Young P.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17167 / STM815.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001043 Genomic DNA. Translation: ACC71760.1.
RefSeqYP_001858806.1. NC_010622.1.

3D structure databases

ProteinModelPortalB2JGX6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391038.Bphy_2588.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACC71760; ACC71760; Bphy_2588.
GeneID6244071.
KEGGbph:Bphy_2588.
PATRIC19191671. VBIBurPhy25146_2746.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAPVIHCPK.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycBPHY391038:GI4Z-2640-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BURP8
AccessionPrimary (citable) accession number: B2JGX6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries