true2009-03-242024-03-2782RUVC_PARP8Complete genome sequence of Burkholderia phymatum STM815(T), a broad host range and efficient nitrogen-fixing symbiont of Mimosa species.Moulin L.Klonowska A.Caroline B.Booth K.Vriezen J.A.Melkonian R.James E.K.Young J.P.Bena G.Hauser L.Land M.Kyrpides N.Bruce D.Chain P.Copeland A.Pitluck S.Woyke T.Lizotte-Waniewski M.Bristow J.Riley M.doi:10.4056/sigs.48610212014Stand. Genomic Sci.9763-774NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]DSM 17167 / CIP 108236 / LMG 21445 / STM815BacteriaRuvCRibonuclease H-like superfamily/Ribonuclease HRuvCRNaseH-like_sfRNaseH_sfX-over_junc_endoDNase_Mg_BSX-over_junc_endoDNase_RuvCruvCCROSSOVER JUNCTION ENDODEOXYRIBONUCLEASE RUVCCROSSOVER JUNCTION ENDODEOXYRIBONUCLEASE RUVCRuvCRSOLVASERUVCRibonuclease H-likeRUVCCrossover junction endodeoxyribonuclease RuvC3.1.21.10Holliday junction nuclease RuvCHolliday junction resolvase RuvCruvCBphy_2554The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair. Endonuclease that resolves HJ intermediates. Cleaves cruciform DNA by making single-stranded nicks across the HJ at symmetrical positions within the homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction. The consensus cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange. HJ branch migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.Binds 2 Mg(2+) ion per subunit.Homodimer which binds Holliday junction (HJ) DNA. The HJ becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it has a different conformation from HJ DNA in complex with RuvA. In the full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ.Belongs to the RuvC family.Crossover junction endodeoxyribonuclease RuvC186491180766138122008-06-10118649100a820a952c32dae88072b9cef2f72bMRILGIDPGLRVTGFGVIDQSGHQLTYVTSGVIKTADADLPTRLGTIFEGISTLIRQHAPDHSAIEKVFVNVNPQSTLLLGQARGAAICGLVSGGVPVAEYTALQLKQAVVGYGRATKEQMQQMVVRLLCLSGIPSTDAADALGMAICHAHGGGTLSTLGGIAPGLAKKGLRVRRGRLVGtrue