B2JGU1 (PUR9_BURP8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
   EC=2.1.2.3
   Alternative name(s):
   AICAR transformylase
2. IMP cyclohydrolase
   EC=3.5.4.10
   Alternative name(s):
   ATIC
   IMP synthase
   Inosinicase

Gene names

Name:	purH
Ordered Locus Names:	Bphy_2553

Organism

Burkholderia phymatum (strain DSM 17167 / STM815) [Complete proteome] [HAMAP]

Taxonomic identifier

391038 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP MF_00139
Sequence similarities	Belongs to the PurH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: EC phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 521	521	Bifunctional purine biosynthesis protein PurH HAMAP MF_00139		PRO_1000096047

Sequences

	Sequence		Length	Mass (Da)
	B2JGU1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 944CFAB78D1A1C74	FASTA	521	56,217
	10 20 30 40 50 60 MIKQALISVS DKSGIVDFAK SLSDLGVKIL STGGTAKLLA DAGLPVTEVA DYTGFPEMLD 70 80 90 100 110 120 GRVKTLHPKV HGGILARRDL PEHMAALEKH DIPTIDLLVV NLYPFVQTVS KEECTLEDAI 130 140 150 160 170 180 ENIDIGGPTM LRSAAKNHRD VTVVVDPADY ATVLDEMRAN GNTVGYKTNF RLATKVFAHT 190 200 210 220 230 240 AQYDGAITNY LTSLTEQLQH RDRNTYPATL NMAFEKVQDL RYGENPHQSA AFYRDIAAPA 250 260 270 280 290 300 GALANYRQLQ GKELSYNNIA DSDAAWECVK TFDAPACVII KHANPCGVAV GANPHEAYSK 310 320 330 340 350 360 AFQTDPTSAF GGIIAFNREV DETAAQAVAK QFVEVLIAPS FSEAAKQLFA AKQNVRLLEI 370 380 390 400 410 420 ALGEGHNAFD LKRVGGGLLV QSLDAKNVQP HELRVVTKRH PTPKEMDDLL FAWRVAKFVK 430 440 450 460 470 480 SNAIVFCANG MTMGVGAGQM SRVDSARIAS IKAQNAGLTL SGTAVASDAF FPFRDGLDVV 490 500 510 520 VNAGATCVIQ PGGSMRDDEV IAAADEHNIA MVVTGIRHFR H

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phymatum STM815." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Bruce D., Goodwin L., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Bacher J., Blanchard J., Cohan F., James E., Lawrence J., Lizotte-Waniewski M., Moulin L., Rainey P., Riley M., Souza V., Wertz J., Young P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17167 / STM815.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001043 Genomic DNA. Translation: ACC71725.1.
RefSeq	YP_001858771.1. NC_010622.1.
3D structure databases
ProteinModelPortal	B2JGU1.
ModBase	Search...
Protein-protein interaction databases
STRING	B2JGU1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6244036.
GenomeReviews	Gene locus Bphy_2553 in contig CP001043_GR.
KEGG	bph:Bphy_2553.
PATRIC	19191599. VBIBurPhy25146_2710.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG498048.
OMA	FTGTRHF.
ProtClustDB	PRK00881.
Family and domain databases
HAMAP	MF_00139. PurH. [Tree]
InterPro	IPR024051. AICAR_Tfase_dom. IPR002695. AICARFT_IMPCHas. IPR016193. Cytidine_deaminase-like. IPR011607. MGS-like_dom. [Graphical view]
Gene3D	G3DSA:3.40.140.20. G3DSA:3.40.140.20. 2 hits. G3DSA:3.40.50.1380. MGS-like_dom. 1 hit.
KO	K00602.
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
SUPFAM	SSF53927. Cytidine_deaminase-like. 1 hit. SSF52335. MGS-like_dom. 1 hit.
TIGRFAMs	TIGR00355. PurH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_BURP8

Accession

Primary (citable) accession number: B2JGU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program