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Protein

NAD kinase

Gene

nadK

Organism
Burkholderia phymatum (strain DSM 17167 / STM815)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751Proton acceptorUniRule annotation
Binding sitei177 – 1771NADUniRule annotation
Binding sitei179 – 1791NADUniRule annotation
Binding sitei214 – 2141NAD; via carbonyl oxygenUniRule annotation
Binding sitei248 – 2481NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 762NADUniRule annotation
Nucleotide bindingi149 – 1502NADUniRule annotation
Nucleotide bindingi190 – 1956NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciBPHY391038:GI4Z-2555-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:Bphy_2504
OrganismiBurkholderia phymatum (strain DSM 17167 / STM815)
Taxonomic identifieri391038 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
ProteomesiUP000001192 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300NAD kinasePRO_1000120836Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi391038.Bphy_2504.

Structurei

3D structure databases

ProteinModelPortaliB2JGE9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227221.
KOiK00858.
OMAiGMVELRV.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

B2JGE9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQANSQFKTV ALVGRSNTPG IGEPLTALAT CIEKLGFEIA FEAETAQEIG
60 70 80 90 100
VSRWPALQPA EIGARADVAI VLGGDGTMLG IGRQLAPYKT PLIGINHGRL
110 120 130 140 150
GFITDIPFSD MREIIPQMLS GSFEREERSL LESRIMRDGQ PIYHALAFND
160 170 180 190 200
VVVNRSGFSG MAELRVSVDG RFMYNQRSDG LIVATPTGST AYALSSQGPI
210 220 230 240 250
LHPQLQGLVL VPIAPHALSN RPIVIPDDSK VSIQIISGRD VNVNFDMQSF
260 270 280 290 300
TALELNDTID VRRSRHTVPF LHPVGYSYYA TLRKKLHWNE YPSHEDDPDD
Length:300
Mass (Da):32,939
Last modified:June 10, 2008 - v1
Checksum:i18F48BC00512A61E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001043 Genomic DNA. Translation: ACC71677.1.
RefSeqiWP_012401881.1. NC_010622.1.
YP_001858723.1. NC_010622.1.

Genome annotation databases

EnsemblBacteriaiACC71677; ACC71677; Bphy_2504.
KEGGibph:Bphy_2504.
PATRICi19191495. VBIBurPhy25146_2658.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001043 Genomic DNA. Translation: ACC71677.1.
RefSeqiWP_012401881.1. NC_010622.1.
YP_001858723.1. NC_010622.1.

3D structure databases

ProteinModelPortaliB2JGE9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391038.Bphy_2504.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC71677; ACC71677; Bphy_2504.
KEGGibph:Bphy_2504.
PATRICi19191495. VBIBurPhy25146_2658.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227221.
KOiK00858.
OMAiGMVELRV.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciBPHY391038:GI4Z-2555-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17167 / STM815.

Entry informationi

Entry nameiNADK_BURP8
AccessioniPrimary (citable) accession number: B2JGE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: May 27, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.