B2JGD2 (PUR5_BURP8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	Bphy_2487

Organism

Burkholderia phymatum (strain DSM 17167 / STM815) [Complete proteome] [HAMAP]

Taxonomic identifier

391038 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_B
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00741_B.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_B		PRO_1000193005

Sequences

	Sequence		Length	Mass (Da)
	B2JGD2 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 38A570284D2DDE6E	FASTA	355	37,173
	10 20 30 40 50 60 MNQPKSAPNS PDSAQGLSYR DAGVDIDAGD ALVDAIKPFA RKTLRDGVLG GIGGFGALFE 70 80 90 100 110 120 VPKKYKEPVL VSGTDGVGTK LKLAFTLNRH DTVGQDLVAM SVNDILVQGA EPLFFLDYFA 130 140 150 160 170 180 CGKLDVGTAA TVVKGIAQGC ELAGCALIGG ETAEMPGMYP DGEYDLAGFA VGAVEKSKII 190 200 210 220 230 240 DGSKIVPGDV VLGLASSGIH SNGYSLVRKI IERAQPDLDA DFDGRTLAEA LMAPTHIYVK 250 260 270 280 290 300 PLLALMQQLE VKGMAHITGG GLVENIPRVL REGLTAELDH RAWPLPPLFA WLQKHGGVAD 310 320 330 340 350 AEMHRVFNCG IGMVVIVSAA DADAATGLLA AAGEQVWKIG TVRQSKEGEA QTVVV

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phymatum STM815." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Bruce D., Goodwin L., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Bacher J., Blanchard J., Cohan F., James E., Lawrence J., Lizotte-Waniewski M., Moulin L., Rainey P., Riley M., Souza V., Wertz J., Young P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17167 / STM815.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001043 Genomic DNA. Translation: ACC71660.1.
RefSeq	YP_001858706.1. NC_010622.1.
3D structure databases
ProteinModelPortal	B2JGD2.
ModBase	Search...
Protein-protein interaction databases
STRING	B2JGD2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6243971.
GenomeReviews	Gene locus Bphy_2487 in contig CP001043_GR.
KEGG	bph:Bphy_2487.
PATRIC	19191459. VBIBurPhy25146_2640.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG531222.
OMA	GIDMIAM.
ProtClustDB	PRK05385.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
KO	K01933.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. PurM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_BURP8

Accession

Primary (citable) accession number: B2JGD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program