B2JF25 (DADA_BURP8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-amino acid dehydrogenase small subunit
EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	Bphy_0765

Organism

Burkholderia phymatum (strain DSM 17167 / STM815) [Complete proteome] [HAMAP]

Taxonomic identifier

391038 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity. HAMAP MF_01202
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity. HAMAP MF_01202
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the DadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000138645
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	B2JF25 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 5699B6C501B030DB	FASTA	428	46,107
	10 20 30 40 50 60 MRVVVLGSGV VGVTSAYYLA RAGHEVTVID REAGPALETS FANAGQISPG YAAPWAAPGV 70 80 90 100 110 120 PLKAVKWMFQ KHAPLAIRLD GTQFQLQWMW QMLQNCTSAR YAVNKGRMVR LAEYSRDCLQ 130 140 150 160 170 180 ALRAETGIQY EGRTGGTLQL FRTQQQLDGA AKDIAVLEEA NVPYELLMPA DLARAEPALA 190 200 210 220 230 240 ATSHKLTGGL RLPGDETGDC QLFTTRLAAL AEQLGVKFRY NTPIDALAME GGRIAGVKCG 250 260 270 280 290 300 NEMVRADNFV VALGSYSTQF LSGLVKIPVY PLKGYSITAP IVDAKSAPVS TVLDETYKIA 310 320 330 340 350 360 ITRFDDRIRV GGMAEIVGFD KKLKQARRET LEMCVNDLFP GGGDTSKATF WTGLRPMTPD 370 380 390 400 410 420 GTPIVGRTPV SNLFLNTGHG TLGWTMSCGS GQLLADLISG KRPAIQSGDL SVHRYLGETA GQTRPAYA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phymatum STM815." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Bruce D., Goodwin L., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Bacher J., Blanchard J., Cohan F., James E., Lawrence J., Lizotte-Waniewski M., Moulin L., Rainey P., Riley M., Souza V., Wertz J., Young P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17167 / STM815.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001043 Genomic DNA. Translation: ACC69954.1.
RefSeq	YP_001857000.1. NC_010622.1.
3D structure databases
ProteinModelPortal	B2JF25.
ModBase	Search...
Protein-protein interaction databases
STRING	B2JF25.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6242263.
GenomeReviews	Gene locus Bphy_0765 in contig CP001043_GR.
KEGG	bph:Bphy_0765.
PATRIC	19187719. VBIBurPhy25146_0808.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG729204.
OMA	VDRQPAV.
ProtClustDB	PRK00711.
Family and domain databases
HAMAP	MF_01202. DadA. [Tree]
InterPro	IPR023080. D-aa_DH_ssu_DadA. IPR006076. FAD-dep_OxRdtase. [Graphical view]
KO	K00285.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA_BURP8

Accession

Primary (citable) accession number: B2JF25

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program